Use of fluorescent probes to detect lipid signaling intermediates in macrophages

Methods in Molecular Biology

Volumn 531, Issue , 2009, Pages 301-328

  Kwok, Vivian ^b   Vachon, Eric ^b   Downey, Gregory P ^a  

^a UNIVERSITY OF TORONTO   (Canada)

^b UNIVERSITY OF COLORADO   (United States)

Author keywords

Confocal microscopy; Fluorescent probes; Green fluorescent protein (GFP); Lipids; Macrophages; Phagocytosis; Phosphoinositides; Protein tyrosine phosphatases (PTP); Signal transduction

Indexed keywords

SCYPHOZOA;

FLUORESCENT DYE; LIPOSOME; NON RECEPTOR PROTEIN TYROSINE PHOSPHATASE; PROTEIN KINASE B; PTPN9 PROTEIN, MOUSE;

ANIMAL; ARTICLE; BIOASSAY; CELL CULTURE; CONFOCAL MICROSCOPY; ENZYMOLOGY; FLUORESCENT ANTIBODY TECHNIQUE; GENETIC PROCEDURES; GENETIC TRANSFECTION; LIPID METABOLISM; MACROPHAGE; METABOLISM; MOUSE; PHAGOCYTOSIS; PHAGOSOME; POLYACRYLAMIDE GEL ELECTROPHORESIS; SIGNAL TRANSDUCTION; WESTERN BLOTTING;

ANIMALS; BIOLOGICAL ASSAY; BLOTTING, WESTERN; CELLS, CULTURED; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FLUORESCENT ANTIBODY TECHNIQUE; FLUORESCENT DYES; LIPID METABOLISM; LIPOSOMES; MACROPHAGES; MICE; MICROSCOPY, CONFOCAL; MOLECULAR PROBE TECHNIQUES; PHAGOCYTOSIS; PHAGOSOMES; PROTEIN TYROSINE PHOSPHATASES, NON-RECEPTOR; PROTO-ONCOGENE PROTEINS C-AKT; SIGNAL TRANSDUCTION; TRANSFECTION;

EID: 65649127767     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-396-7_19     Document Type: Article

References (25)

1. Aderem, A. and D.M. Underhill. (1999) Mechanisms of phagocytosis in macrophages. Annu Rev Immunol. 17 : 593-623 (Pubitemid 29241135)
2. Greenberg, S. (2001) Diversity in phagocytic signalling. J Cell Sci. 114 (Pt 6) : 1039-1040 (Pubitemid 32288518)
3. May, R.C. (2001) Phagocytosis in C. elegans: CED-1 reveals its secrets. Trends Cell Biol. 11 (4) : 150 (Pubitemid 32295917)
4. May, R.C. and L.M. (2001) Machesky, Phagocytosis and the actin cytoskeleton. J Cell Sci. 114 (Pt 6) : 1061-1077
5. Gaudry, M., et al. (1992) Evidence for the involvement of tyrosine kinases in the locomotory responses of human neutrophils. J Leukoc Biol. 51 (2) : 103-108
6. Grinstein, S. and W. Furuya. (1991) Tyrosine phosphorylation and oxygen consumption induced by G proteins in neutrophils. Am J Physiol. 260 (5 Pt 1) : C1019-C1027
7. Hunter, T. (1995) Protein kinases and phosphatases: the yin and yang of protein phosphorylation and signaling. Cell. 80 (2) : 225-236
8. Kruger, J., et al. (2000) Deficiency of Src homology 2-containing phosphatase 1 results in abnormalities in murine neutrophil function: studies in motheaten mice. J Immunol. 165 (10) : 5847-5859
9. Tonks, N.K. and B.G. (1996) Neel, From form to function: signaling by protein tyrosine phosphatases. Cell. 87 (3) : 365-368 (Pubitemid 26374311)
10. Gu, M., I. Warshawsky, and P.W. Majerus. (1992) Cloning and expression of a cytosolic megakaryocyte protein-tyrosine-phosphatase with sequence homology to retinaldehyde-binding protein and yeast SEC14p. Proc Natl Acad Sci U S A. 89 (7) : 2980-2984
11. Huynh, H., et al. (2003) Homotypic secretory vesicle fusion induced by the protein tyrosine phosphatase MEG2 depends on polyphosphoinositides in T cells. J Immunol. 171 (12) : 6661-6671 (Pubitemid 37532098)
12. Kruger, J.M., et al. (2002) Protein-tyrosine phosphatase MEG2 is expressed by human neutrophils. localization to the phagosome and activation by polyphosphoinositides. J Biol Chem. 277 (4) : 2620-2628 (Pubitemid 34953287)
13. Botelho, R.J., et al. (2000) Localized biphasic changes in phosphatidylinositol-4,5-bisphosphate at sites of phagocytosis. J Cell Biol. 151 (7) : 1353-1368
14. Sechi, A.S. and J. Wehland. (2000) The actin cytoskeleton and plasma membrane connection: PtdIns(4,5)P(2) influences cytoskeletal protein activity at the plasma membrane. J Cell Sci. 113 Pt 21 : 3685-3695
15. Vieira, O.V., et al. (2001) Distinct roles of class I and class III phosphatidylinositol 3-kinases in phagosome formation and maturation. J Cell Biol. 155 (1) : 19-25 (Pubitemid 34286204)
16. Vachon, E., Kwok, V., et al. Protein-tyrosine-phosphatase- MEG2 regulation by phosphatidylinositol- 3,4,5-trisphosphate and recruitment to nascent phagosomes (unpublished data)
17. Gerdes, H.H. and C. Kaether. (1996) Green fluorescent protein: applications in cell biology. FEBS Lett. 389 (1) : 44-47 (Pubitemid 26227423)
18. Pelham, H.R. (1996) The dynamic organisation of the secretory pathway. Cell Struct Funct. 21 (5) : 413-419 (Pubitemid 27068328)
19. Montalibet, J., K.I. Skorey, and B.P. Kennedy. (2005) Protein tyrosine phosphatase: enzymatic assays. Methods. 35 (1) : 2-8 (Pubitemid 39647573)
20. Shirai, T., et al. (1998) Specific detection of phosphatidylinositol 3,4,5-trisphosphate binding proteins by the PIP3 analogue beads: an application for rapid purification of the PIP3 binding proteins. Biochim Biophys Acta. 1402 (3) : 292-302 (Pubitemid 28206924)
21. Vachon, E., et al. (2006) CD44 is a phagocytic receptor. Blood. 107 (10) : 4149-4158 (Pubitemid 43726826)
22. Lutz, D.A., X.M. Chen, and B.J. McLaughlin. (1993) Isolation of the phagocytic compartment from macrophages using a paramagnetic, particulate ligand. Anal Biochem. 214 (1) : 205-211 (Pubitemid 23300375)
23. Saito, K., L. Tautz, and T. Mustelin. (2007) The lipid-binding SEC14 domain. Biochim Biophys Acta. 1771 (6) : 719-726 (Pubitemid 46856890)
24. Huynh, K.K., et al. (2007) LAMP proteins are required for fusion of lysosomes with phagosomes. EMBO J. 26 (2) : 313-324 (Pubitemid 46160933)
25. Garrido, C., et al. (2006) Mechanisms of cytochrome c release from mitochondria. Cell Death Differ. 13 (9) : 1423-1433 (Pubitemid 44210346)