Use of GFP to investigate expression of plant-derived vaccines

Methods in Molecular Biology

Volumn 515, Issue , 2009, Pages 275-285

  Li, Hong Ye ^a   Chye, Mee Len ^a  

^a UNIVERSITY OF HONG KONG   (Hong Kong)

Author keywords

Autofluorescent protein tag; Plant bioreactors; Plant transformation; Recombinant subunit vaccines; Severe acute respiratory syndrome coronavirus; Subcellular localization

Indexed keywords

CAPSID PROTEIN; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; VACCINE;

ARTICLE; GENE EXPRESSION; GENE VECTOR; GENETICS; IMMUNOLOGY; METABOLISM; REPORTER GENE; SARS CORONAVIRUS; TOBACCO;

CAPSID PROTEINS; GENE EXPRESSION; GENES, REPORTER; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; RECOMBINANT FUSION PROTEINS; SARS VIRUS; TOBACCO; VACCINES;

EID: 65649123087     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-559-6_19     Document Type: Article

References (14)

1. Walmsley, A.M. and Arntzen, C.J. (2003) Plant cell factories and mucosal vaccines. Curr. Opin. Biotechnol. 14, 145-150. (Pubitemid 36561227)
2. Porgrebnyak, N., Golovkin, M., Andrianov, V., Spitsin, S., Smirnov, Y., Egolf, R., and Koprowski, H. (2005) Severe acute respiratory syndrome (SARS) S protein production in plants: development of recombinant vaccine. Proc. Natl. Acad. Sci. USA 102, 9062-9067. (Pubitemid 40881078)
3. Dantuma, N.P., Lindsten, K., Glas, R., Jellne, M., and Masucci, M.G. (2000) Short-lived green fluorescent proteins for quantifying ubiquitin "proteasome-dependent proteolysis in living cells ". Nat. Biotechnol. 18, 538-543. (Pubitemid 30313959)
4. Kloetzel, P.M. (2004) Generation of major histocompatibility complex class I antigens: functional interplay between proteasomes and TPPII. Nat. Immunol. 5 (7), 661-669. (Pubitemid 39023294)
5. Gelvin, S.B. (2003) Agrobacterium-mediated plant transformation: the biology behind the "gene-jockeying" tool. Microbiol. Mol. Biol. Rev. 67, 16-37. (Pubitemid 36314782)
6. Yoshioka, Y., Takahashi, Y., Matsuoka, K., Nakamura, K., Koizumi, J., Kojima, M., and Machida, Y. (1996) Transient gene expression in plant cells mediated by Agrobacterium tumefaciens: application for the analysis of virulence loci. Plant Cell Physiol. 37, 782-789. (Pubitemid 26405174)
7. Schob, H., Kunz, C.,and Meins, F., Jr. (1997) Silencing of transgenes introduced into leaves by agroinfiltration: a simple, rapid method for investigation of sequence requirements for gene silencing. Mol. Gen. Genet. 256, 581-585.
8. Kapila, J., De Rycke, R., Van Montagu, M., and Angenon, G. (1997) An Agrobacterium-mediated transient gene expression for intact leaves. Plant Sci. 122, 101-108.
9. Goodin, M.M., Dietzgen, R.G., Schichnes, D., Ruzin, S., and Jackson, A.O. (2002) pGD vectors: versatile tools for the expression of green and red fluorescent protein fusions in agroinfiltrated plant leaves. Plant J. 31, 375-383. (Pubitemid 34915408)
10. Hoekema, A., Hirsch, P.R., Hooykas, P.J.J., and Schilperoort, R.A. (1983) A binary plant vector strategy based on separation of vir and T-region of the A. tumefaciens Ti plasmid. Nature 303, 179-180. (Pubitemid 13081082)
11. Poon, L.L.M., Wong, O.K., Luk, W., Yuen, K.Y., Peiris, J.S.M., and Guan, Y. (2003) Rapid diagnosis of a coronas associated with severe acute respiratory syndrome (SARS). Clin. Chem. 49, 953-955. (Pubitemid 36623476)
12. Li, H.Y., Ramalingam, S., and Chye, M.L. (2006) Accumulation of recombinant SARSCoV spike protein in plant cytosol and chloroplasts indicate potential for development of plant-derived oral vaccines. Exp. Biol. Med. 231, 1346-1352. (Pubitemid 44330570)
13. Gorlich, D. and Mattaj, I.W. (1996) Nucleocytoplasmic transport. Science 271, 1513-1518. (Pubitemid 26097209)
14. Shin, D.-I. and Park, H.-S. (2005) Transient expression in Chinese Cabbage by hydrogen peroxide-aided agroinfiltration. Agric. Chem. Biotechnol. 4, 229-230.