Injection of Parhyale hawaiensis blastomeres with fluorescently labeled tracers

Cold Spring Harbor Protocols

Volumn 4, Issue 1, 2009, Pages

  Rehm, E Jay ^a   Hannibal, Roberta L ^a   Chaw, Crystal R ^a   Vargas Vila, Mario A ^a   Patel, Nipam H ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOTIN; FLUORESCEIN ISOTHIOCYANATE DEXTRAN; FLUORESCENT DYE; MESSENGER RNA; TRACER;

AMPHIPODA; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BLASTOMERE; CELL LINEAGE; CONTROLLED STUDY; DEVELOPMENTAL STAGE; EMBRYO; EMBRYO CELL; EMBRYO DEVELOPMENT; EMBRYONAL TISSUE; FEMALE; IN SITU HYBRIDIZATION; MICRODISSECTION; NONHUMAN; OOCYTE CLEAVAGE; PROTEIN LOCALIZATION; RNA PROBE; TISSUE FIXATION; TRANSPOSON;

PARHYALE;

EID: 65649120769     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5128     Document Type: Article

References (8)

1. Bevis, B.J. and Glick, B.S. 2001. Rapidly maturing variants of the Discosoma red fluorescent protein (DsRed). Nat. Biotechnol 20: 83-87.
2. Gerberding, M., Browne, W.E., and Patel, N.H. 2002. Cell lineage analysis of the amphipod crustacean Parhyale hawaiensis reveals an early restriction of cell fates. Development 129: 5789-5801.
3. Krieg, P.A. and Melton, D.A. 1984. Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs. Nucleic Acid Res 12: 7057-7070.
4. Pavlopoulos, A. and Averof, M. 2005. Establishing genetic transformation for comparative developmental studies in the crustacean Parhyale hawaiensis. Proc. Natl. Acad. Sci. 102: 7888-7893.
5. Rehm, E.J., Hannibal, R.L., Chaw, R.C., Vargas-Vila, M.A., and Patel, N.H. 2009a. Fixation and dissection of Parhyale hawaiensis embryos. Cold Spring Harb. Protoc. (this issue). doi: 10.1101/ pdb.prot5127.
6. Rehm, E.J., Hannibal, R.L., Chaw, R.C., Vargas-Vila, M.A., and Patel, N.H. 2009b. Antibody staining of Parhyale hawaiensis embryos. Cold Spring Harb. Protoc. (this issue). doi: 10.1101/pdb.prot5129.
7. Rehm, E.J., Hannibal, R.L., Chaw, R.C., Vargas-Vila, M.A., and Patel, N.H. 2009c. In situ hybridization of labeled RNA probes to fixed Parhyale hawaiensis embryos. Cold Spring Harb. Protoc. (this issue). doi: 10.1101/pdb.prot5130.
8. Rehm, E.J., Hannibal, R.L., Chaw, R.C., Vargas-Vila, M.A., and Patel, N.H. 2009d. The crustacean Parhyale hawaiensis: A new model for arthropod development. Cold Spring Harb. Protoc. (this issue). doi: 10.1101/pdb.emo114.