A new resorufin-based α-glucosidase assay for high-throughput screening

Analytical Biochemistry

Volumn 390, Issue 1, 2009, Pages 79-84

  Motabar, Omid ^a   Shi, Zhen Dan ^b   Goldin, Ehud ^a   Liu, Ke ^a   Southall, Noel ^a   Sidransky, Ellen ^a   Austin, Christopher P ^a   Griffiths, Gary L ^b   Zheng, Wei ^a  

^a NATIONAL HUMAN GENOME RESEARCH INSTITUTE   (United States)

^b NATIONAL HEART LUNG AND BLOOD INSTITUTE   (United States)

Author keywords

Assay miniaturization; Assay optimization; Enzyme assay; High throughput screening (HTS); Pompe disease; Glucosidase

Indexed keywords

DISEASE CONTROL; ENZYMES;

ASSAY OPTIMIZATION; ENZYME ASSAYS; GLUCOSIDASE; HIGH-THROUGHPUT SCREENING; POMPE DISEASE;

DIAGNOSIS;

4 METHYLUMBELLIFERYL ALPHA DEXTRO GLUCOPYRANOSIDE; ALPHA GLUCOSIDASE; FLUORESCENT DYE; RESORUFIN ALPHA DEXTRO GLUCOPYRANOSIDE; RESORUFIN DERIVATIVE; UNCLASSIFIED DRUG;

ARTICLE; ENZYME ASSAY; ENZYME KINETICS; ENZYME MECHANISM; ENZYME SUBSTRATE; FLUORESCENCE ANALYSIS; HIGH THROUGHPUT SCREENING; PKA; PRIORITY JOURNAL;

EID: 65649118646     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2009.04.010     Document Type: Article

References (24)

1. Hirschhorn R., and Reuser A.J.J. Glycogen storage disease type II: acid (α-glucosidase (acid maltase) deficiency. In: Scriver C.R., Beaudet A.L., Sly W.S., and Valle D. (Eds). The Metabolic and Molecular Bases of Inherited Disease (2001), McGraw-Hill, New York 3389-3420
2. Martiniuk F., Chen A., Mack A., Arvanitopoulos E., Chen Y., Rom W.N., Codd W.J., Hanna B., Alcabes P., Raben N., and Plotz P. Carrier frequency for glycogen storage disease type II in New York and estimates of affected individuals born with the disease. Am. J. Med. Genet. 79 (1998) 69-72
3. Raben N., Plotz P., and Byrne B.J. Acid α-glucosidase deficiency (glycogenosis type II, Pompe disease). Curr. Mol. Med. 2 (2002) 145-166
4. Montalvo A.L., Cariati R., Deganuto M., Guerci V., Garcia R., Ciana G., Bembi B., and Pittis M.G. Glycogenosis type II: identification and expression of three novel mutations in the acid α-glucosidase gene causing the infantile form of the disease. Mol. Genet. Metab. 81 (2004) 203-208
5. Hermans M.M., van Leenen D., Kroos M.A., Beesley C.E., Van der Ploeg A.T., Sakuraba H., Wevers R., Kleijer W., Michelakakis H., Kirk E.P., Fletcher J., Bosshard N., Basel-Vanagaite L., Besley G., and Reuser A.J. Twenty-two novel mutations in the lysosomal α-glucosidase gene (GAA) underscore the genotype-phenotype correlation in glycogen storage disease type II. Hum. Mutat. 23 (2004) 47-56
6. Reuser A.J., Kroos M., Willemsen R., Swallow D., Tager J.M., and Galjaard H. Clinical diversity in glycogenosis type II: biosynthesis and in situ localization of acid α-glucosidase in mutant fibroblasts. J. Clin. Invest. 79 (1987) 1689-1699
7. Reuser A.J., Kroos M., Oude Elferink R.P., and Tager J.M. Defects in synthesis, phosphorylation, and maturation of acid α-glucosidase in glycogenosis type II. J. Biol. Chem. 260 (1985) 8336-8341
8. Parenti G., Zuppaldi A., Gabriela Pittis M., Rosaria Tuzzi M., Annunziata I., Meroni G., Porto C., Donaudy F., Rossi B., Rossi M., Filocamo M., Donati A., Bembi B., Ballabio A., and Andria G. Pharmacological enhancement of mutated α-glucosidase activity in fibroblasts from patients with Pompe disease. Mol. Ther. 15 (2007) 508-514
9. Okumiya T., Kroos M.A., Vliet L.V., Takeuchi H., Van der Ploeg A.T., and Reuser A.J. Chemical chaperones improve transport and enhance stability of mutant α-glucosidases in glycogen storage disease type II. Mol. Genet. Metab. 90 (2007) 49-57
10. Hultberg B., Sjoblad S., and Ockerman P.A. Glycosidases in human skin fibroblast cultures: α-fucosidase, α-galactosidase, α-glucosidase, α-mannosidase, and N-acetyl-glucosaminidase. Acta Paediatr. Scand. 64 (1975) 123-131
11. Okumiya T., Keulemans J.L., Kroos M.A., Van der Beek N.M., Boer M.A., Takeuchi H., Van Diggelen O.P., and Reuser A.J. A new diagnostic assay for glycogen storage disease type II in mixed leukocytes. Mol. Genet. Metab. 88 (2006) 22-28
12. Porter W.H., Jennings Jr. C.D., and Wilson H.D. Measurement of α-glucosidase activity in serum from patients with cystic fibrosis or pancreatitis. Clin. Chem. 32 (1986) 652-656
13. Simeonov A., Jadhav A., Thomas C.J., Wang Y., Huang R., Southall N.T., Shinn P., Smith J., Austin C.P., Auld D.S., and Inglese J. Fluorescence spectroscopic profiling of compound libraries. J. Med. Chem. 51 (2008) 2363-2371
14. Mahmoud A.M., Geslevich J., Kint J., Depuydt C., Huysse L., Zalata A., and Comhaire F.H. Seminal plasma α-glucosidase activity and male infertility. Hum. Reprod. 13 (1998) 591-595
15. Guerin J.F., Ali H.B., Rollet J., Souchier C., and Czyba J.C. α-Glucosidase as a specific epididymal enzyme marker: its validity for the etiologic diagnosis of azoospermia. J. Androl. 7 (1986) 156-162
16. Walker J.M., Winder J.S., and Kellam S.J. High-throughput microtiter plate-based chromogenic assays for glycosidase inhibitors. Appl. Biochem. Biotechnol. 38 (1993) 141-146
17. Mazura P., Fohlerova R., Brzobohaty B., Kiran N.S., and Janda L. A new sensitive method for enzyme kinetic studies of scarce glucosides. J. Biochem. Biophys. Methods 68 (2006) 55-63
18. Hays W.S., Wheeler D.E., Eghtesad B., Glew R.H., and Johnston D.E. Expression of cytosolic β-glucosidase in guinea pig liver cells. Hepatology 28 (1998) 156-163
19. Parzefall W., Berger W., Kainzbauer E., Teufelhofer O., Schulte-Hermann R., and Thurman R.G. Peroxisome proliferators do not increase DNA synthesis in purified rat hepatocytes. Carcinogenesis 22 (2001) 519-523
20. Courtin-Duchateau M., and Veyrières A. Synthesis of 4-methylumbelliferyl 1,2-cis-glycosides. Carbohydr. Res. 65 (1978) 23-33
21. Yamada R., Negoro N., Bessho K., and Yamada K. Metallic samarium and iodine in alcohol: deacylation and dealkyloxycarbonylation of protected alcohols and lactams. Synlett 26 (1995) 1261-1263
22. Bueno C., Villegas M.L., Bertolotti S.G., Previtali C.M., Neumann M.G., and Encinas M.V. The excited-state interaction of resazurin and resorufin with amines in aqueous solutions: photophysics and photochemical reactions. Photochem. Photobiol. 76 (2002) 385-390
23. Coleman D.J., Studler M.J., and Naleway J.J. A long-wavelength fluorescent substrate for continuous fluorometric determination of cellulase activity: resorufin-β-d-cellobioside. Anal. Biochem. 371 (2007) 146-153
24. Inglese J., Auld D.S., Jadhav A., Johnson R.L., Simeonov A., Yasgar A., Zheng W., and Austin C.P. Quantitative high-throughput screening: a titration-based approach that efficiently identifies biological activities in large chemical libraries. Proc. Natl. Acad. Sci. USA 103 (2006) 11473-11478