Improved semiquantitative Western blot technique with increased quantification range

Journal of Immunological Methods

Volumn 345, Issue 1-2, 2009, Pages 40-48

  Heidebrecht, F ^a   Heidebrecht, A ^b   Schulz, I ^a   Behrens, S E ^c   Bader, A ^a  

^a UNIVERSITY OF LEIPZIG   (Germany)

^b GERMAN AEROSPACE CENTER DLR   (Germany)

^c MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

Author keywords

Calibration curve; Hyperbolic regression; Semiquantitative Western blot

Indexed keywords

ARTICLE; CALIBRATION; CHEMOLUMINESCENCE; ENZYME LINKED IMMUNOSORBENT ASSAY; KINETICS; MATHEMATICAL MODEL; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; WESTERN BLOTTING;

ANIMALS; BLOTTING, WESTERN; CALIBRATION; ELECTROCHEMICAL TECHNIQUES; KINETICS; MICE; MODELS, CHEMICAL; PROTEINS; SENSITIVITY AND SPECIFICITY; SOFTWARE;

EID: 65649114739     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2009.03.018     Document Type: Article

References (21)

1. Blomberg J., and Klasse P.J. Quantification of immunoglobulin on electrophoretic immunoblot strips as a tool for human immunodeficiency virus serodiagnosis. Journal of Clinical Microbiology 26 1 (1988) 111
2. Braitbard O., Glickstein H., Bishara-Shieban J., Pace U., and Stein W.D. Competition between bound and free peptides in an ELISA-based procedure that assays peptides derived from protein digests. Proteome Science 4 12 (2006)
3. Burnette W.N. "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Analytical Biochemistry 112 (1981) 195
4. Chen E., Kwon Y.T., Lim M.S., Dube I.D., and Hough M.R. Loss of Ubr1 promotes aneuploidy and accelerates B-cell lymphomagenesis in TLX1/HOX11-transgenic mice. Oncogene 25 (2006) 5725
5. Chen J., Guerriero E., Lathrop K., and SundarRaj N. Rho/ROCK signaling in regulation of corneal epithelial cell cycle progression. IOVS 49 1 (2008) 175
6. Debeverec P.E., and Malik J. Recovering high dynamic range radiance maps from photographs, SIGGRAPH '97. Proceedings of the 24th Annual Conference on Computer Graphics and Interactive Techniques (1997) 369
7. Ekins R., and Gosling J.P. Theoretical aspects of saturation analysis. In: Diczfalusy E. (Ed). Karolinska Symposia on Research Methods in Reproductive Endocrinology: Steroid Assay by Protein Binding (1970) 11
8. Englert C.R., and Harlander J.M. Flatfielding in spatial heterodyne spectroscopy. Applied Optics 45 19 (2006) 4583
9. Feather-Henigan K. Immunoblot imaging with a cooled CCD camera and chemiluminescent substrates. American Biotechnology Laboratory 17 (1999) 44
10. Holland T., and Holland H. A mathematical model of immunohistochemical preparations, which provides quantitative predictions. Journal of Microscopy 214 (2003) 1
11. Kim T.H., Huh J.H., Lee S., Kang H., Kim G.I., and An H.J. Down-regulation of claudin-2 in breast is associated with advanced disease. Histopathology 53 1 (2008) 48
12. Kricka L.J. Chemiluminescent and bioluminestcent techniques. Clinical Chemistry 37/9 (1991) 1472
13. Lin J.C., Choi E., and Pagano J.S. Qualitative and quantitative analyses of Epstein-Barr virus early antigen diffuse component by western blotting enzyme-linked immunosorbent assay with a monoclonal antibody. Journal of Virology 53 (1985) 793
14. Michaelis L., and Menten M.L. Die Kinetik der Invertinwirkung. Biochemische Zeitschrift 49 (1913) 339
15. O'Connor T., and Gosling J.P. The dependence of radioimmunoassay detection limits on antibody affinity. Journal of Immunological Methods 208 (1997) 181
16. Ornberg R.L., Harper T.F., and Liu H. Western blot analysis with quantum dot fluorescence technology: a sensitive and quantitative method for multiplexed proteomics. Nature Methods 2 1 (2005) 79
17. Renart J., Reiser J., and Stark G.R. Transfer of proteins from gels to diazobenzyloxymethyl-paper and detection with antisera: a method for studying antibody specificity and antigen structure. Proceedings of the National Academy of Sciences 76 7 (1979) 3116
18. Roman-Blas J.A., Stokes D.G., and Jimenez S.A. Modulation of TGF-b signaling by proinflammatory cytokinesin articular chondrocytes. Osteoarthritis and Cartilage 15 (2007) 1367
19. Schilling M., et al. Computational processing and error reduction strategies for standardized quantitative data in biological networks. FEBS Journal 272 (2005) 6400
20. Studnika G.M. Hyperbolic regression analysis for kinetics, electrophoresis, ELISA, RIA, Bradford, Lowry, and other applications. CABIOS 3 1 (1987) 9
21. Towbin H., Staehelin T., and Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. PNAS 76 9 (1979) 4350