A mathematical model of immunohistochemical preparations, which provides quantitative predictions

Journal of Microscopy

Volumn 214, Issue 1, 2004, Pages 1-6

  Holland, T ^a   Holland, H ^a  

^a NONE   (Sweden)

Author keywords

Affinity constant; Dissociation constant; Immunohistochemistry; Law of Mass Action; Quantification; Sensitivity; Specificity

Indexed keywords

ANTIBODIES; DISSOCIATION;

AFFINITY CONSTANTS; DISSOCIATION CONSTANT; HISTOLOGICAL SECTION; IMMUNOHISTOCHEMISTRY; LAW OF MASS ACTION; PROPERTY; QUANTIFICATION; QUANTITATIVE PREDICTION; SENSITIVITY; SPECIFICITY;

EPITOPES;

ANTIBODY; EPITOPE;

ARTICLE; CALCULATION; CONCENTRATION RESPONSE; DISSOCIATION CONSTANT; HISTOLOGY; IMMUNOHISTOCHEMISTRY; MASS ACTION; MATHEMATICAL MODEL; PREDICTION; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REACTION ANALYSIS; SIGNAL DETECTION; STAINING;

EID: 1842506572     PISSN: 00222720     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.0022-2720.2004.01294.x     Document Type: Article

References (6)

1. Getzoff, E.D., Trainer, J. & Lerner, R.A. (1988) The chemistry and mechanism of antibody binding to protein antigens. Adv. Immunol. 43, 1-98.
2. McCormick, M., Yu, C., Hobbs, C. & Hall, P.A. (1993) The relevance of antibody concentration to the immunohistological quantification of cell proliferation-associated antigens. Histopathology, 22, 543-547.
3. Owen, M. & Steward, M. (1998) Antigen recognition. Immunology, 5th edn (ed. by M. Roitt, J. Brostoff and D. Male), pp. 107-119. Mosby, London.
4. Rang, H.P., Dale, M.M. & Ritter, J.M. (1995) Pharmacology, 3rd edn. Churchill Livingstone, Edinburgh.
5. Rice, J.A. (1995) Mathematical Statistics and Data Analysis. Duxbury Press, Belmont, CA.
6. Steward, M. & Male, D. (1998) Immunological techniques. Immunology, 5th edn (ed. by M. Roitt, J. Brostoff and D. Male), pp. 331-495. Mosby, London.