Long-term leucine supplementation does not increase muscle mass or strength in healthy elderly men

American Journal of Clinical Nutrition

Volumn 89, Issue 5, 2009, Pages 1468-1475

  Verhoeven, Suzanne ^a   Vanschoonbeek, Kristof ^a   Verdijk, Lex B ^a   Koopman, René ^a   Wodzig, Will K W H ^b   Dendale, Paul ^c   Van Loon, Luc J C ^a  

^a MAASTRICHT UNIVERSITY   (Netherlands)

^b MAASTRICHT UNIVERSITY MEDICAL CENTER   (Netherlands)

^c VIRGA JESSE HOSPITAL   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

HEMOGLOBIN A1C; HIGH DENSITY LIPOPROTEIN CHOLESTEROL; ISOLEUCINE; LEUCINE; LOW DENSITY LIPOPROTEIN CHOLESTEROL; MYOSIN HEAVY CHAIN; PLACEBO; TRIACYLGLYCEROL; VALINE; GLYCOSYLATED HEMOGLOBIN; INSULIN; MUSCLE PROTEIN;

AGED; AMINO ACID BLOOD LEVEL; ARTICLE; BODY COMPOSITION; CHOLESTEROL BLOOD LEVEL; CLINICAL TRIAL; COMPUTER ASSISTED TOMOGRAPHY; CONTROLLED CLINICAL TRIAL; CONTROLLED STUDY; CREATININE CLEARANCE; DIET SUPPLEMENTATION; DOUBLE BLIND PROCEDURE; DUAL ENERGY X RAY ABSORPTIOMETRY; GLYCEMIC CONTROL; HUMAN; INSULIN SENSITIVITY; MALE; MUSCLE MASS; MUSCLE STRENGTH; NORMAL HUMAN; RANDOMIZED CONTROLLED TRIAL; TRIACYLGLYCEROL BLOOD LEVEL; BLOOD; BODY MASS; DRUG EFFECT; GLUCOSE BLOOD LEVEL; HISTOLOGY; METABOLISM; PATIENT SELECTION; PHOTON ABSORPTIOMETRY; PHYSIOLOGY; RADIOGRAPHY; SKELETAL MUSCLE;

ABSORPTIOMETRY, PHOTON; AGED; BLOOD GLUCOSE; BODY MASS INDEX; DIETARY SUPPLEMENTS; HEMOGLOBIN A, GLYCOSYLATED; HUMANS; INSULIN; LEUCINE; MALE; MUSCLE PROTEINS; MUSCLE STRENGTH; MUSCLE, SKELETAL; PATIENT SELECTION; PLACEBOS; TOMOGRAPHY, X-RAY COMPUTED;

EID: 65549171522     PISSN: 00029165     EISSN: None     Source Type: Journal    
DOI: 10.3945/ajcn.2008.26668     Document Type: Article

References (39)

1. Evans WJ. What is sarcopenia? J Gerontol A Biol Sci Med Sci 1995;50: 5-8.
2. Frontera WR, Hughes VA, Fielding RA, Fiatarone MA, Evans WJ, Roubenoff R. Aging of skeletal muscle: a 12-yr longitudinal study. J Appl Physiol 2000;88:1321-1326 (Pubitemid 30217433)
3. Short KR, Vittone JL, Bigelow ML, Proctor DN, Nair KS. Age and aerobic exercise training effects on whole body and muscle protein metabolism. Am J Physiol Endocrinol Metab 2004;286:E92-101.
4. Volpi E, Mittendorfer B, Rasmussen BB, Wolfe RR. The response of muscle protein anabolism to combined hyperaminoacidemia and glucose-induced hyperinsulinemia is impaired in the elderly. J Clin Endocrinol Metab 2000;85:4481-4490 (Pubitemid 32157622)
5. Balagopal P, Ljungqvist O, Nair KS. Skeletal muscle myosin heavy-chain synthesis rate in healthy humans. Am J Physiol 1997;272:E45-50.
6. Hasten DL, Pak-Loduca J, Obert KA, Yarasheski KE. Resistance exercise acutely increases MHC and mixed muscle protein synthesis rates in 78-84 and 23-32 yr olds. Am J Physiol Endocrinol Metab 2000;278: E620-6. (Pubitemid 30215171)
7. Yarasheski KE, Welle S, Nair KS. Muscle protein synthesis in younger and older men. JAMA 2002;287:317-318
8. Katsanos CS, Kobayashi H, Sheffield-Moore M, Aarsland A, Wolfe RR. Aging is associated with diminished accretion of muscle proteins after the ingestion of a small bolus of essential amino acids. Am J Clin Nutr 2005;82:1065-1073 (Pubitemid 43779766)
9. Paddon-Jones D, Sheffield-Moore M, Katsanos CS, Zhang XJ, Wolfe RR. Differential stimulation of muscle protein synthesis in elderly humans following isocaloric ingestion of amino acids or whey protein. Exp Gerontol 2006;41:215-219 (Pubitemid 43165694)
10. Volpi E, Sheffield-Moore M, Rasmussen BB, Wolfe RR. Basal muscle amino acid kinetics and protein synthesis in healthy young and older men. JAMA 2001;286:1206-1212 (Pubitemid 33015550)
11. Cuthbertson D, Smith K, Babraj J, et al. Anabolic signaling deficits underlie amino acid resistance of wasting, aging muscle. FASEB J 2005; 19:422-424 (Pubitemid 40316537)
12. Katsanos CS, Kobayashi H, Sheffield-Moore M, Aarsland A, Wolfe RR. A high proportion of leucine is required for optimal stimulation of the rate of muscle protein synthesis by essential amino acids in the elderly. Am J Physiol Endocrinol Metab 2006;291:E381-7. (Pubitemid 44035298)
13. Rieu I, Balage M, Sornet C, et al. Leucine supplementation improves muscle protein synthesis in elderly men independently of hyperaminoacidaemia. J Physiol 2006;575:305-315 (Pubitemid 44182713)
14. Combaret L, Dardevet D, Rieu I, et al. A leucine-supplemented diet restores the defective postprandial inhibition of proteasome-dependent proteolysis in aged rat skeletal muscle. J Physiol 2005;569:489-499 (Pubitemid 41830117)
15. Louard RJ, Barrett EJ, Gelfand RA. Overnight branched-chain amino acid infusion causes sustained suppression of muscle proteolysis. Metabolism 1995;44:424-429
16. Norton LE, Layman DK. Leucine regulates translation initiation of protein synthesis in skeletal muscle after exercise. J Nutr 2006;136:533S-7S.
17. Alberti KG, Zimmet PZ. Definition, diagnosis and classification of diabetes mellitus and its complications. Part 1: diagnosis and classification of diabetes mellitus provisional report of a WHO consultation. Diabet Med 1998;15:539-553
18. Jones PR, Pearson J. Anthropometric determination of leg fat and muscle plus bone volumes in young male and female adults. J Physiol 1969;204:63P-6P.
19. Mayhew JL, Prinster JL,Ware JS, Zimmer DL, Arabas JR, Bemben MG. Muscular endurance repetitions to predict bench press strength in men of different training levels. J Sports Med Phys Fitness 1995;35:108-113
20. Verdijk LB, Koopman R, Schaart G, Meijer K, Savelberg HH, van Loon LJ. Satellite cell content is specifically reduced in type II skeletal muscle fibers in the elderly. Am J Physiol Endocrinol Metab 2007;292:E151-7. (Pubitemid 46052937)
21. Mitsiopoulos N, Baumgartner RN, Heymsfield SB, Lyons W, Gallagher D, Ross R. Cadaver validation of skeletal muscle measurement by magnetic resonance imaging and computerized tomography. J Appl Physiol 1998;85:115-122 (Pubitemid 28334913)
22. Matthews DR, Hosker JP, Rudenski AS, Naylor BA, Treacher DF, Turner RC. Homeostasis model assessment: insulin resistance and betacell function from fasting plasma glucose and insulin concentrations in man. Diabetologia 1985;28:412-419 (Pubitemid 15018832)
23. Esmarck B, Andersen JL, Olsen S, Richter EA, Mizuno M, Kjaer M. Timing of postexercise protein intake is important for muscle hypertrophy with resistance training in elderly humans. J Physiol 2001;535: 301-311 (Pubitemid 32782746)
24. Rennie MJ. A role for leucine in rejuvenating the anabolic effects of food in old rats. J Physiol 2005;569:357.
25. Dardevet D, Sornet C, Balage M, Grizard J. Stimulation of in vitro rat muscle protein synthesis by leucine decreases with age. J Nutr 2000; 130:2630-2635 (Pubitemid 30815736)
26. Fiatarone MA, Marks EC, Ryan ND, Meredith CN, Lipsitz LA, Evans WJ. High-intensity strength training in nonagenarians. Effects on skeletal muscle. JAMA 1990;263:3029-3034
27. Kimball SR, Jefferson LS. Regulation of protein synthesis by branched-chain amino acids. Curr Opin Clin Nutr Metab Care 2001;4:39-43. (Pubitemid 34754478)
28. van Loon LJC. Amino acids as pharmaco-nutrients for the treatment of type 2 diabetes. Immun Endocrinol Metab Agents in Med. Chem 2007; 7:39-48. (Pubitemid 46613390)
29. Manders RJ, Koopman R, Sluijsmans WE, et al. Co-ingestion of a protein hydrolysate with or without additional leucine effectively reduces postprandial blood glucose excursions in type 2 diabetic men. J Nutr 2006;136:1294-1299
30. Manders RJ, Wagenmakers AJ, Koopman R, et al. Co-ingestion of a protein hydrolysate and amino acid mixture with carbohydrate improves plasma glucose disposal in patients with type 2 diabetes. Am J Clin Nutr 2005;82:76-83. (Pubitemid 41716380)
31. Manders RJ, Praet SF, Meex RC, et al. Protein hydrolysate/leucine co-ingestion reduces the prevalence of hyperglycemia in type 2 diabetic patients. Diabetes Care 2006;29:2721-2722
32. Zhang Y, Guo K, LeBlanc RE, Loh D, Schwartz GJ, Yu YH. Increasing dietary leucine intake reduces diet-induced obesity and improves glucose and cholesterol metabolism in mice via multimechanisms. Diabetes 2007;56:1647-1654 (Pubitemid 46842599)
33. Matthews DE. Observations of branched-chain amino acid administration in humans. J Nutr 2005;135:1580S-4S.
34. Campbell WW, Crim MC, Dallal GE, Young VR, Evans WJ. Increased protein requirements in elderly people: new data and retrospective reassessments. Am J Clin Nutr 1994;60:501-509
35. Campbell WW, Evans WJ. Protein requirements of elderly people. Eur J Clin Nutr 1996;50(suppl 1):S180-3 (discussion S183-5).
36. Campbell WW, Johnson CA, McCabe GP, Carnell NS. Dietary protein requirements of younger and older adults. Am J Clin Nutr 2008;88: 1322-1329
37. Campbell WW, Trappe TA, Wolfe RR, Evans WJ. The recommended dietary allowance for protein may not be adequate for older people to maintain skeletal muscle. J Gerontol A Biol Sci Med Sci 2001;56: M373-80.
38. Rand WM, Pellett PL, Young VR. Meta-analysis of nitrogen balance studies for estimating protein requirements in healthy adults. Am J Clin Nutr 2003;77:109-127
39. Trumbo P, Schlicker S, Yates AA, Poos M. Dietary reference intakes for energy, carbohydrate, fiber, fat, fatty acids, cholesterol, protein and amino acids. J Am Diet Assoc 2002;102:1621-1630 (Pubitemid 40434081)