Static light scattering from concentrated protein solutions II: Experimental test of theory for protein mixtures and weakly self-associating proteins

Biophysical Journal

Volumn 96, Issue 5, 2009, Pages 1992-1998

  Fernández, Cristina ^a   Minton, Allen P ^a  

^a NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BOVINE SERUM ALBUMIN; CHYMOTRYPSIN A; DIMER; MONOMER; OVALBUMIN; OVOMUCOID; PROTEIN; ALPHA-CHYMOTRYPSIN; CHYMOTRYPSIN;

ARTICLE; EXPERIMENTATION; LIGHT SCATTERING; MOLECULE; PH; PROTEIN BINDING; THERMODYNAMICS; ANIMAL; CATTLE; CHEMICAL MODEL; CHEMISTRY; CHICKEN; FEMALE; LIGHT; RADIATION SCATTERING;

BOVINAE;

ANIMALS; CATTLE; CHICKENS; CHYMOTRYPSIN; FEMALE; HYDROGEN-ION CONCENTRATION; LIGHT; MODELS, CHEMICAL; OVALBUMIN; OVOMUCIN; SCATTERING, RADIATION; SERUM ALBUMIN, BOVINE;

EID: 65549164345     PISSN: 00063495     EISSN: 15420086     Source Type: Journal    
DOI: 10.1016/j.bpj.2008.11.054     Document Type: Article

References (36)

1. Zhou, H. -X., G. Rivas, and A. P. Minton. 2008. Macromolecular crowding and confinement: biochemical, biophysical and potential physiological consequences. Ann. Rev. Biophys. 37:375-397.
2. Zimmerman, S. B., and A. P. Minton. 1993. Macromolecular crowding: biochemical, biophysical, and physiological consequences. Annu. Rev. Biophys. Biomol. Struct. 22:27-65.
3. Shire, S. J., Z. Shahrokh, and J. Liu. 2004. Challenges in the development of high protein concentration formulations. J. Pharm. Sci. 93:1390-1402.
4. Ross, P. D., and A. P. Minton. 1977. Analysis of nonideal behavior in concentrated hemoglobin solutions. J. Mol. Biol. 112:437-452.
5. Minton, A. P. 2007. Static light scattering from concentrated protein solutions I. General theory for protein mixtures and application to self-associating proteins. Biophys. J. 93:1321-1328.
6. Minton, A. P. 2007. The effective hard particle model provides a simple, robust, and broadly applicable description of nonideal behavior in concentrated solutions of bovine serum albumin and other nonassociating proteins. J. Pharm. Sci. 96:3466-3469.
7. Minton, A. P. 2008. Effective hard particle model for the osmotic pressure of highly concentrated binary protein solutions. Biophys. J. 94:L57-L59.
8. Minton, A. P., and H. Edelhoch. 1982. Light scattering of bovine serum albumin solutions: extension of the hard particle model to allow for electrostatic repulsion. Biopolymers. 21:451-458.
9. Rivas, G., J. A. Fernandez, and A. P. Minton. 1999. Direct observation of the self-association of dilute proteins in the presence of inert macromolecules at high concentration via tracer sedimentation equilibrium: theory, experiment, and biological significance. Biochemistry. 38:9379-9388.
10. Ross, P. D., R. W. Briehl, and A. P. Minton. 1978. Temperature dependence of nonideality in concentrated solutions of hemoglobin. Biopolymers. 17:2285-2288.
11. Minton, A. P. 1995. A molecular model for the dependence of the osmotic pressure of bovine serum albumin upon concentration and pH. Biophys. Chem. 57:65-70.
12. Fernández, C., and A. P. Minton. 2008. Automated measurement of the static light scattering of macromolecular solutions over a broad range of concentrations. Anal. Biochem. 381:254-257.
13. Morimoto, K., and G. Kegeles. 1967. Dimerization and activity of chymotrypsin at pH 4. Biochemistry. 6:3007-3010.
14. Aune, K., and S. N. Timasheff. 1971. Dimerization of alpha-chymotrypsin I. pH dependence in the acid region. Biochemistry. 10:1609-1617.
15. Kameyama, K., and A. P. Minton. 2006. Rapid quantitative characterization of protein interactions by composition gradient static light scattering. Biophys. J. 90:2164-2169.
16. 1% values for proteins at selected wavelengths in the ultraviolet and visible region. In Handbook of Biochemistry and Molecular Biology. G. D. Fasman, editor. CRC Press, Cleveland, pp. 383-545.
17. Davis, J. G., C. J. Mapes, and J. W. Donovan. 1971. Batch purification of ovomucoid and characterization of the purified product. Biochemistry. 10:39-42.
18. Attri, A. K., and A. P. Minton. 2005. New methods for measuring macromolecular interactions in solution via static light scattering: basic methodology and application to nonassociating and self-associating proteins. Anal. Biochem. 337:103-110.
19. Theisen, A., C. Johann, M. P. Deacon, and S. E. Harding. 2000. Refractive Increment Data-book. Nottingham University Press, Nottingham. 55.
20. Stacey, K. A. 1956. Light-scattering in physical chemistry. Academic Press, New York.
21. Stockmayer, W. H. 1950. Light scattering in multi-component systems. J. Chem. Phys. 18:58-61.
22. Asthagiri, D., A. Paliwal, D. Abras, A. M. Lenhoff, and M. E. Paulitis. 2005. A consistent experimental and modeling approach to light-scattering studies of protein-protein interactions in solution. Biophys. J. 88:3300-3309.
23. Winzor, D. J., M. Deszczynski, S. E. Harding, and P. R. Wills. 2007. Nonequivalence of second virial coefficients from sedimentation equilibrium and static light scattering studies of protein solutions. Biophys. Chem. 128:46-55.
24. Hall, D., and A. P. Minton. 2003. Macromolecular crowding: qualitative and semiquantitative successes, quantitative challenges. Biochem. Biophys. Res. Commun. 1649:127-139.
25. Boublík, T. 1974. Statistical thermodynamics of convex molecule fluids. Mol. Phys. 27:1415-1427.
26. Lebowitz, J. L., E. Helfand, and E. Praestgaard. 1965. Scaled particle theory of fluid mixtures. J. Chem. Phys. 43:774-779.
27. Hill, T. L., and Y. -D. Chen. 1973. Theory of aggregation in solution I. General equations and application to the stacking of bases, nucleosides, etc. Biopolymers. 12:1285-1312.
28. Muramatsu, N., and A. P. Minton. 1989. Hidden self-association of proteins. J. Mol. Recognit. 1:166-171.
29. Bajaj, H., V. K. Sharma, and D. S. Kalonia. 2007. A high-throughput method for detection of protein self-association and second virial coefficient using size-exclusion chromatography through simultaneouls measurement of concentration and scattered light intensity. Pharm. Res. 24:2071-2083.
30. Alford, J. R., S. C. Kwok, J. N. Roberts, D. S. Wuttke, B. S. Kendrick, et al. 2008. High concentration formulations of recombinant human interleukin-1 receptor antagonist: I. Physical characterization. J. Pharm. Sci. 97:3035-3050.
31. Zorrilla, S., M. Jiménez, P. Lillo, G. Rivas, and A. P. Minton. 2004. Sedimentation equilibrium in a solution containing an arbitrary number of solute species at arbitrary concentrations: theory and application to concentrated solutions of ribonuclease. Biophys. Chem. 108:89-100.
32. Jiménez, M., G. Rivas, and A. P. Minton. 2007. Quantitative characterization of weak self-association in concentrated solutions of Immunoglobulin G via the measurement of sedimentation equilibrium and osmotic pressure. Biochemistry. 46:8373-8378.
33. Pandit, M. W., and M. S. Narasinga Rao. 1974. Studies on self-association of proteins. The self-association of alpha-chymotrypsin at pH 8.3 and ionic strength 0.05. Biochemistry. 13:1048-1055.
34. Ackers, G. K., and T. E. Thompson. 1965. Determination of stoichiometry and equilibrium constants for reversibly associating systems by molecular sieve chromatography. Proc. Natl. Acad. Sci. USA. 53:342-349.
35. Malamud, D., and J. W. Drysdale. 1978. Isoelectric points of proteins: a table. Anal. Biochem. 86:620-647.
36. Rhodes, M. B., P. R. Azari, and R. E. Feeney. 1958. Analysis, fractionation and purification of egg white proteins with cellulose-cation exchanger. J. Biol. Chem. 230:399-408.