Molecular characterization of rhodococcus equi from horse-breeding farms by means of multiplex PCR for the vap gene family

Current Microbiology

Volumn 58, Issue 4, 2009, Pages 399-403

  Monego, Fernanda ^a   Maboni, Franciele ^a   Krewer, Cristina ^b   Vargas, Agueda ^a   Costa, Mateus ^b   Loreto, Elgion ^a  

^a FEDERAL UNIVERSITY OF SANTA MARIA   (Brazil)

^b UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCO   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BREEDING; FECES ANALYSIS; FUNGAL GENE; FUNGAL STRAIN; FUNGUS ISOLATION; GENE IDENTIFICATION; GENE SEQUENCE; HORSE; METHODOLOGY; MULTIGENE FAMILY; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RHODOCOCCUS EQUI; SOIL ANALYSIS; TECHNIQUE; VIRULENCE; ACTINOMYCETALES INFECTION; ANIMAL; ANIMAL DISEASE; BRAZIL; EPIDEMIOLOGY; GENETICS; HORSE DISEASE; HUMAN; ISOLATION AND PURIFICATION; MANURE; MICROBIOLOGY; PATHOGENICITY;

EQUIDAE; RHODOCOCCUS EQUI;

BACTERIAL PROTEIN; MEMBRANE PROTEIN; VIRULENCE FACTOR;

ACTINOMYCETALES INFECTIONS; ANIMALS; BACTERIAL PROTEINS; BRAZIL; BREEDING; EPIDEMIOLOGY, MOLECULAR; HORSE DISEASES; HORSES; HUMANS; MANURE; MEMBRANE GLYCOPROTEINS; MULTIGENE FAMILY; POLYMERASE CHAIN REACTION; RHODOCOCCUS EQUI; SOIL MICROBIOLOGY; VIRULENCE; VIRULENCE FACTORS;

EID: 65549157604     PISSN: 03438651     EISSN: 14320991     Source Type: Journal    
DOI: 10.1007/s00284-009-9370-6     Document Type: Article

References (29)

1. KS Bell JC Philip N Christofi DWJ Aw 1996 Identification of Rhodococcus equi using the polymerase chain reaction Lett Appl Microbiol 23 72 74 (Pubitemid 26337019)
2. BA Byrne JF Prescott GH Palmer 2001 Virulence plasmid of Rhodococcus equi contains inducible gene family encoding secreted proteins Infect Immun 69 650 656 (Pubitemid 32109522)
3. CH Cantor BA Byrne SA Hines HM Richards 1998 Vap-A negative Rhodococcus equi in a dog with necrotizing pyogranulomatous hepatitis, osteomyelitis, and myositis J Vet Diagn Invest 10 297 300 (Pubitemid 128657093)
4. S Giguèrre MK Hondalus JA Yager 1999 Role of the 85 Kb plasmid and plasmid encoded virulence protein A in intracellular survival and virulence of R. equi Infect Immun 67 3548 3557 (Pubitemid 29291528)
5. ND Halbert RA Reitzel RJ Martens ND Cohen 2005 Evaluation of a multiplex polymerase chain reaction assay for simultaneous detection of Rhodococcus equi and the vapA gene Am J Vet Res 66 1380 1385 (Pubitemid 41613991)
6. MK Hondalus 1997 Rhodococcus equi: pathogenesis and virulence Vet Microbiol 56 257 268 (Pubitemid 27339155)
7. MK Hondalus DM Mosser 1994 Survival and replication of Rhodococcus equi in macrophages Infect Immun 62 4167 4175 (Pubitemid 24301939)
8. S Jacks S Giguérre JF Prescott 2007 In vivo expression of and cell-mediated immune responses to the plasmid-encoded virulence-associated proteins of Rhodococcus equi in foals Clin vac immun 14 369 374 (Pubitemid 46732546)
9. S Jain BR Bloom MK Hondalus 2003 Deletion of vapA encoding virulence associated protein A attenuates the intracellular actinomycete Rhodococcus equi Mol Microbiol 50 115 128 (Pubitemid 37222791)
10. CC Krewer DA Spricigo SA Botton 2008 Molecular characterization of Rhodococcus equi isolates of horse breeding farms from endemic region in south of Brazil by multiplex PCR Braz J Microbiol 39 1 6
11. A Lazzari AC Vargas V Dutra 1997 Aspectos epidemiológicos do Rhodococcus equi em eqüinos do município de Bagé, RS, Brasil Cien Rural 27 441 446
12. L Makrai S Takai M Tamura 2002 Characterization of virulence plasmid types in Rhodococcus equi isolates from foals, pigs, humans and soil in Hungary Vet Microbiol 88 377 384 (Pubitemid 35245922)
13. FS Mizuno M Sakamoto MK Yoshikawa 2005 VapB-positive Rhodococcus equi infection in an HIV-infected patient in Japan J Infect Chemother 11 37 40 (Pubitemid 40379480)
14. G Muscatello GA Anderson JR Gilkerson GF Browning 2006 Associations between the ecology of virulent Rhodococcus equi and the epidemiology of R. equi pneumonia on Australian thoroughbred farms Appl Environ Microbiol 72 6152 6160 (Pubitemid 44414648)
15. F Napoleão PV Damasco TCF Camello 2005 Pyogenic liver abscess due to Rhodococcus equi in an immunocompetent host J Clin Microbiol 43 1002 1004 (Pubitemid 40256084)
16. C Oldfield H Bonella L Renwick 2004 Rapid determination of vapA/vapB genotype in Rhodococcus equi using a differential polymerase chain reaction method Antonie Van Leeuwenhoek 85 317 326 (Pubitemid 38461065)
17. Y Pei C Dupont T Sydor 2006 Cholesterol oxidase (ChoE) is not important in the virulence of Rhodococcus equi Vet Microbiol 118 240 246
18. MG Ribeiro I Seki K Yasuoka 2005 Molecular epidemiology of virulent Rhodococcus equi from foals in Brazil: virulence plasmids of 85-kb type I, and a new variant, 87-kb Type III Comp Immunol Microbiol Infect Dis 28 53 61 (Pubitemid 39618886)
19. Sambrook R, Russel DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, New York
20. SAS Institute. SAS/STAT User's guide, Version 8. Cary, 2000 1383p
21. R Staden KF Beal JK Bonfield 2000 The staden package Methods Mol Biol 132 115 130
22. T Sydor Bargen Kv U Becken 2008 A mycolyl transferase mutant of Rhodococcus equi lacking capsule integrity is fully virulent Vet Microbiol 128 327 341
23. S Takai T Sekisaki A Osawa 1991 Association between a large plasmid and 15-17 kDa antigens in virulent Rhodococcus equi Infect Immun 59 4056 4060
24. S Takai T Ikeda Y Sasaki 1995 Identification of virulent Rhodococcus equi by amplification of gene coding 15-to 17-Kilodalton antigens J Clin Microbiol 33 1624 1627
25. S Takai Y Imai N Fukunaga 1995 Identification of virulence-associated antigens and plasmids in Rhodococcus equi from patients with AIDS J Infect Dis 172 1306 1311
26. S Takai N Fukunaga S Ochiai 1996 Isolation of virulent and intermediately virulent R. equi from soil and sand on parks and yards in Japan J Vet Med Sci 58 669 672 (Pubitemid 126485705)
27. S Takai N Fukunaga S Ochiai 1996 Identification of intermediately virulent Rhodococcus equi isolates from pig J Clin Microbiol 34 1034 1037 (Pubitemid 26091538)
28. S Takai AS Hines T Sekizaki 2000 DNA sequence and comparison of virulence plasmids from Rhodococcus equi ATCC 33701 and 103 Infect Immun 68 6840 6847 (Pubitemid 32463392)
29. S Takai T Anzai Y Fujita 2000 Pathogenicity of Rhodococcus equi expressing a virulence-associated 20 kDa protein (VapB) in foals Vet Microbiol 76 71 80 (Pubitemid 30484048)