Generation of cre recombinase-expressing transgenic mice using bacterial artificial chromosomes

Methods in Molecular Biology

Volumn 530, Issue , 2009, Pages 325-342

  Parkitna, Jan Rodriguez ^a   Engblom, David ^a   Schütz, Günther ^a  

^a GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

Author keywords

bacterial artificial chromosome; conditional gene deletion; Cre recombinase; Cre loxP; recombineering; tamoxifen

Indexed keywords

ANIMALIA; BACTERIA (MICROORGANISMS); MUS; MUS MUSCULUS;

CRE RECOMBINASE; INTEGRASE;

ANIMAL; ARTICLE; BACTERIAL ARTIFICIAL CHROMOSOME; FEMALE; GENETIC RECOMBINATION; GENETICS; MALE; METABOLISM; MOUSE; PHYSIOLOGY; TRANSGENE; TRANSGENIC MOUSE;

ANIMALS; CHROMOSOMES, ARTIFICIAL, BACTERIAL; FEMALE; INTEGRASES; MALE; MICE; MICE, TRANSGENIC; RECOMBINATION, GENETIC; TRANSGENES;

EID: 65549115554     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-471-1_17     Document Type: Article

References (23)

1. Abremski K, Hoess R, Sternberg N. Studies on the properties of P1 site-specific recombination: evidence for topologically unlinked products following recombination. Cell 1983; 32:1301-11. (Pubitemid 13033058)
2. Gu H, Marth JD, Orban PC, Mossmann H, Rajewsky K. Deletion of a DNA polymerase beta gene segment in T cells using cell typespecific gene targeting. Science 1994; 265:103-6.
3. Thomas KR, Capecchi MR. Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells. Cell 1987; 51:503-12.
4. Branda CS, Dymecki SM. Talking about a revolution: the impact of site-specific recombinases on genetic analyses in mice. Dev Cell 2004; 6:7-28. (Pubitemid 38089202)
5. Dymecki SM, Kim JC. Molecular neuroanatomy's "Three Gs": a primer. Neuron 2007; 54:17-34.
6. Feil R, Brocard J, Mascrez B, LeMeur M, Metzger D, Chambon P. Ligand-activated site-specific recombination in mice. Proc Natl Acad Sci USA 1996; 93: 10887-90. (Pubitemid 26333086)
7. Zhang Y, Riesterer C, Ayrall AM, Sablitzky F, Littlewood TD, Reth M. Inducible sitedirected recombination in mouse embryonic stem cells. Nucleic Acids Res 1996; 24:543-8. (Pubitemid 26085826)
8. Brocard J,Warot X, WendlingO, et al. Spatiotemporally controlled site-specific somatic mutagenesis in the mouse. Proc Natl Acad Sci USA 1997; 94:14559-63. (Pubitemid 28041413)
9. Logie C, Stewart AF. Ligand-regulated sitespecific recombination. Proc Natl Acad Sci USA 1995; 92:5940-4.
10. Burke DT, Carle GF, Olson MV. Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors. Science 1987; 236:806-12. (Pubitemid 17071153)
11. Schedl A, Beermann F, Thies E, Montoliu L, Kelsey G, Schutz G. Transgenic mice generated by pronuclear injection of a yeast artificial chromosome. Nucleic Acids Res 1992; 20:3073-7.
12. Shizuya H, Birren B, Kim UJ, et al. Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector. Proc Natl Acad Sci USA 1992; 89:8794-7.
13. Yang XW, Model P, Heintz N. Homologous recombination based modification in Escherichia coli and germline transmission in transgenic mice of a bacterial artificial chromosome. Nat Biotechnol 1997; 15:859-65.
14. Zhang Y, Buchholz F, Muyrers JP, Stewart AF. A new logic for DNA engineering using recombination in Escherichia coli. Nat Genet 1998; 20:123-8. (Pubitemid 28455443)
15. Yu D, Ellis HM, Lee EC, Jenkins NA, Copeland NG, Court DL. An efficient recombination system for chromosome engineering in Escherichia coli. Proc Natl Acad Sci USA 2000; 97:5978-83. (Pubitemid 30367509)
16. Angrand PO, Daigle N, van der Hoeven F, Scholer HR, Stewart AF. Simplified generation of targeting constructs using ET recombination. Nucleic Acids Res 1999; 27:e16.
17. Liu P, Jenkins NA, Copeland NG. A highly efficient recombineering-based method for generating conditional knockout mutations. Genome Res 2003; 13:476-84. (Pubitemid 36395405)
18. Muyrers JP, Zhang Y, Testa G, Stewart AF. Rapid modification of bacterial artificial chromosomes by ET-recombination. Nucleic Acids Res 1999; 27:1555-7. (Pubitemid 29206301)
19. Warming S, Costantino N, Court DL, Jenkins NA, Copeland NG. Simple and highly efficient BAC recombineering using galK selection. Nucleic Acids Res 2005; 33:e36.
20. Copeland NG, Jenkins NA, Court DL. Recombineering: a powerful new tool for mouse functional genomics. Nat Rev Genet 2001; 2:769-79. (Pubitemid 33685574)
21. Parlato R, Otto C, Begus Y, Stotz S, Schutz G. Specific ablation of the transcription factor CREB in sympathetic neurons surprisingly protects against developmentally regulated apoptosis. Development 2007; 134:1663-70. (Pubitemid 46796563)
22. Shimshek DR, Kim J, Hubner MR, et al. Codon-improved Cre recombinase (iCre) expression in the mouse. Genesis 2002; 32:19-26. (Pubitemid 34117128)
23. Feil R, Wagner J, Metzger D, Chambon P. Regulation of Cre recombinase activity by mutated estrogen receptor ligand-binding domains. Biochem Biophys Res Commun 1997; 237:752-7. (Pubitemid 27434760)