A single EBV-based vector for stable episomal maintenance and expression of GFP in human embryonic stem cells

Regenerative Medicine

Volumn 4, Issue 2, 2009, Pages 239-250

  Thyagarajan, Bhaskar ^a   Scheying, Kelly ^a   Xue, Haipeng ^a   Fontes, Andrew ^a   Chesnut, Jon ^a   Rao, Mahendra ^a   Lakshmipathy, Uma ^a  

^a Life Technologies   (United States)

Author keywords

Adult stem cells; Episomal vectors; Epstein Barr virus nuclear antigen protein; Human embryonic stem cells; Nonintegrating vector systems; Stable gene expression

Indexed keywords

EPSTEIN BARR VIRUS ANTIGEN; GREEN FLUORESCENT PROTEIN; VIRUS VECTOR; VIRUS PROTEIN;

ADIPOCYTE; ANTIGEN EXPRESSION; ARTICLE; CARTILAGE CELL; CELL DIFFERENTIATION; EMBRYONIC STEM CELL; EPSTEIN BARR VIRUS; GENE CASSETTE; GENE REPLICATION; HUMAN; HUMAN CELL; MESENCHYMAL STEM CELL; NONHUMAN; OSTEOBLAST; PLASMID; PRIORITY JOURNAL; VIRAL GENE DELIVERY SYSTEM; CYTOLOGY; GENE VECTOR; GENETIC TRANSDUCTION; GENETICS; METABOLISM; METHODOLOGY; TRANSGENE;

EMBRYONIC STEM CELLS; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HERPESVIRUS 4, HUMAN; HUMANS; PLASMIDS; TRANSDUCTION, GENETIC; TRANSGENES; VIRAL PROTEINS;

EID: 65549096752     PISSN: 17460751     EISSN: None     Source Type: Journal    
DOI: 10.2217/17460751.4.2.239     Document Type: Article

References (41)

1. Braam SR, Denning C, van den Brink S et al.: Improved genetic manipulation of human embryonic stem cells. Nat. Methods 5, 389-392 (2008).
2. Oka M, Chang LJ, Costantini F, Terada N: Lentiviral vector-mediated gene transfer in embryonic stem cells. Methods Mol. Biol. 329, 273-281 (2006).
3. Oshimura M, Katoh M: Transfer of human artificial chromosome vectors into stem cells. Reprod. Biomed. Online 16, 57-69 (2008).
4. Thyagarajan B, Liu Y, Shin S et al.: Creation of engineered human embryonic stem cell lines using phiC31 integrase. Stem Cells 26, 119-126 (2008).
5. Vallier L, Alexander M, Pedersen R: Conditional gene expression in human embryonic stem cells. Stem Cells 25, 1490-1497 (2007).
6. Wilber A, Linehan JL, Tian X et al.: Efficient and stable transgene expression in human embryonic stem cells using transposon-mediated gene transfer. Stem Cells 25, 2919-2927 (2007).
7. Di Domenico AI, Christodoulou I, Pells SC, McWhir J, Thomson AJ: Sequential genetic modification of the hprt locus in human ESCs combining gene targeting and recombinase-mediated cassette exchange. Cloning Stem Cells 10, 217-230 (2008).
8. Yates F, Daley GQ: Progress and prospects: gene transfer into embryonic stem cells. Gene Ther. 13, 1431-1439 (2006).
9. Zwaka TP, Thomson JA: Homologous recombination in human embryonic stem cells. Nat. Biotechnol. 21, 319-321 (2003).
10. Eiges R: Genetic manipulation of human embryonic stem cells by transfection. Methods Mol. Biol. 331, 221-239 (2006).
11. Lakshmipathy U, Pelacho B, Sudo K et al.: Efficient transfection of embryonic and adult stem cells. Stem Cells 22, 531-543 (2004).
12. Mohr JC, de Pablo JJ, Palecek SP: Electroporation of human embryonic stem cells: small and macromolecule loading and DNA transfection. Biotechnol. Prog. 22, 825-834 (2006).
13. Gropp M, Reubinoff B: Lentiviral vector-mediated gene delivery into human embryonic stem cells. Methods Enzymol. 420, 64-81 (2006).
14. Jang JE, Shaw K, Yu XJ et al.: Specific and stable gene transfer to human embryonic stem cells using pseudotyped lentiviral vectors. Stem Cells Dev. 15, 109-117 (2006).
15. Kim S, Kim GJ, Miyoshi H et al.: Efficiency of the elongation factor-1α promoter in mammalian embryonic stem cells using lentiviral gene delivery systems. Stem Cells Dev. 16, 537-545 (2007).
16. Vieyra DS, Goodell MA: Pluripotentiality and conditional transgene regulation in human embryonic stem cells expressing insulated tetracycline-ON transactivator. Stem Cells 25, 2559-2566 (2007).
17. al Yacoub N, Romanowska M, Haritonova N, Foerster J: Optimized production and concentration of lentiviral vectors containing large inserts. J. Gene Med. 9, 579-584 (2007).
18. Smith JR, Maguire S, Davis LA et al.: Robust, persistent transgene expression in human embryonic stem cells is achieved with AAVS1-targeted integration. Stem Cells 26, 496-504 (2008).
19. Cooper MJ, Miron S: Efficient episomal expression vector for human transitional carcinoma cells. Hum. Gene Ther. 4, 557-566 (1993).
20. Westphal EM, Sierakowska H, Livanos E, Kole R, Vos JM: A system for shuttling 200-kb BAC/PAC clones into human cells: stable extrachromosomal persistence and long-term ectopic gene activation. Hum. Gene Ther. 9, 1863-1873 (1998).
21. Belt PB, Groeneveld H, Teubel WJ, van de Putte P, Backendorf C: Construction and properties of an Epstein-Barr-virus-derived cDNA expression vector for human cells. Gene 84, 407-417 (1989).
22. James MR, Stary A, Daya-Grosjean L, Drougard C, Sarasin A: Comparative study of Epstein-Barr virus and SV40-based shuttle-expression vectors in human repair-deficient cells. Mutat. Res. 220, 169-185 (1989).
23. Mazda O: Improvement of nonviral gene therapy by Epstein-Barr virus (EBV)-based plasmid vectors. Curr. Gene Ther. 2, 379-392 (2002).
24. Stoll SM, Sclimenti CR, Baba EJ, Meuse L, Kay MA, Calos MP: Epstein-Barr virus/human vector provides high-level, long-term expression of α1-antitrypsin in mice. Mol. Ther. 4, 122-129 (2001).
25. van Craenenbroeck K, Vanhoenacker P, Haegeman G: Episomal vectors for gene expression in mammalian cells. Eur. J. Biochem. 267, 5665-5678 (2000).
26. Wade-Martins R, Frampton J, James MR: Long-term stability of large insert genomic DNA episomal shuttle vectors in human cells. Nucleic Acids Res. 27, 1674-1682 (1999).
27. Lupton S, Levine AJ: Mapping genetic elements of Epstein-Barr virus that facilitate extrachromosomal persistence of Epstein-Barr virus-derived plasmids in human cells. Mol. Cell. Biol. 5, 2533-2542 (1985).
28. Mecsas J, Sugden B: Replication of plasmids derived from bovine papilloma virus type 1 and Epstein-Barr virus in cells in culture. Annu. Rev. Cell Biol. 3, 87-108 (1987).
29. Yates JL, Warren N, Sugden B: Stable replication of plasmids derived from Epstein-Barr virus in various mammalian cells. Nature 313, 812-815 (1985).
30. Yates JL, Guan N: Epstein-Barr virus-derived plasmids replicate only once per cell cycle and are not amplified after entry into cells. J. Virol. 65, 483-488 (1991).
31. Ren CP, Zhao M, Shan WJ et al.: Establishment of human embryonic stem cell line stably expressing Epstein-Barr virus-encoded nuclear antigen 1. Acta Biochim. Biophys. Sin. (Shanghai) 37, 68-73 (2005).
32. Ren C, Zhao M, Yang X et al.: Establishment and applications of Epstein-Barr virus-based episomal vectors in human embryonic stem cells. Stem Cells 24, 1338-1347 (2006).
33. Kameda T, Smuga-Otto K, Thomson JA: A severe de novo methylation of episomal vectors by human ES cells. Biochem. Biophys. Res. Commun. 349, 1269-1277 (2006).
34. Sasaki Y, Sone T, Yahata K et al.: Multigene gateway clone design for expression of multiple heterologous genes in living cells: Eukaryotic clones containing two and three ORF multigene cassettes expressed from a single promoter. J. Biotechnol. 136(3-4), 103-112 (2008).
35. Sasaki Y, Sone T, Yoshida S et al.: Evidence for high specificity and efficiency of multiple recombination signals in mixed DNA cloning by the Multisite Gateway system. J. Biotechnol. 107, 233-243 (2004).
36. Sone T, Yahata K, Sasaki Y et al.: Multi-gene gateway clone design for expression of multiple heterologous genes in living cells: Modular construction of multiple cDNA expression elements using recombinant cloning. J. Biotechnol. 136(3-4), 113-121 (2008).
37. Yahata K, Kishine H, Sone T et al.: Multigene gateway clone design for expression of multiple heterologous genes in living cells: conditional gene expression at near physiological levels. J. Biotechnol. 118, 123-134 (2005).
38. Zeng X, Chen J, Liu Y et al.: BG01V: a variant human embryonic stem cell line which exhibits rapid growth after passaging and reliable dopaminergic differentiation. Restor. Neurol. Neurosci. 22, 421-428 (2004).
39. Nordhoff V, Hubner K, Bauer A, Orlova I, Malapetsa A, Scholer HR: Comparative ana lysis of human, bovine, and murine Oct-4 upstream promoter sequences. Mamm. Genome 12, 309-317 (2001).
40. Yeom YI, Fuhrmann G, Ovitt CE et al.: Germline regulatory element of Oct-4 specific for the totipotent cycle of embryonal cells. Development 122, 881-894 (1996).
41. Wilson JB, Bell JL, Levine AJ: Expression of Epstein-Barr virus nuclear antigen-1 induces B cell neoplasia in transgenic mice. EMBO J. 15, 3117-3126 (1996).