Application of an asymmetric helical tube reactor for fast identification of gene transcripts of pathogenic viruses by micro flow-through PCR

Biomedical Microdevices

Volumn 11, Issue 3, 2009, Pages 685-692

  Hartung, R ^a,b   Brosing A ^a,f   Sczcepankiewicz, G ^c   Liebert, U ^c   Hafner N ^b   Durst M ^b   Felbel, J ^d,g   Lassner, D ^e   Kohler J M ^a  

^a ILMENAU UNIVERSITY OF TECHNOLOGY   (Germany)

^b JENA UNIVERSITY HOSPITAL   (Germany)

^c UNIVERSITY OF LEIPZIG   (Germany)

^d LEIBNIZ INSTITUTE OF PHOTONIC TECHNOLOGY   (Germany)

^e Institut Kardiale Diagnostik und Therapie GmbH Berlin   (Germany)

^f SENCKENBERG RESEARCH INSTITUTE   (Germany)

^g Sepsis diagnostics expert group   (Germany)

Author keywords

Flow through process; Fluid segment technique; Micro reactor; Rapid PCR; Reverse transcription; Virus diagnostic

Indexed keywords

FLOW-THROUGH PROCESS; FLUID SEGMENT TECHNIQUE; MICRO REACTOR; RAPID PCR; REVERSE TRANSCRIPTION;

AMPLIFICATION; NUCLEIC ACIDS;

TRANSCRIPTION;

COMPLEMENTARY DNA; MESSENGER RNA; POLITEF; VIRUS DNA; VIRUS RNA;

ARTICLE; DNA DETERMINATION; GENE AMPLIFICATION; GENETIC TRANSCRIPTION; HUMAN; HUMAN CELL; MEASLES VIRUS; MICROFLUIDICS; NONHUMAN; POLYMERASE CHAIN REACTION; PORTABLE EQUIPMENT; PRIORITY JOURNAL; REVERSE TRANSCRIPTION; TUBULAR REACTOR; VIRAL GENETICS; VIRUS IDENTIFICATION; WART VIRUS;

BACTERIOPHAGE LAMBDA; BIOREACTORS; CELL LINE, TUMOR; EQUIPMENT DESIGN; FEMALE; HUMAN PAPILLOMAVIRUS 16; HUMANS; MEASLES; NUCLEIC ACID AMPLIFICATION TECHNIQUES; POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TEMPERATURE; TIME FACTORS; TRANSCRIPTION, GENETIC;

HUMAN PAPILLOMAVIRUS; MEASLES VIRUS;

EID: 65449117600     PISSN: 13872176     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10544-008-9280-6     Document Type: Article

References (24)

1. P.A. Auroux, Y. Koc, A. deMello, A. Manz, P.J. Day, Lab Chip 4, 534 (2004). doi: 10.1039/b408850f
2. F.A. Ausubel, R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith, K. Struhl (eds.), "Current Protocols in Molecular Biology", Wiley, New York (1998)
3. J.R. Burns, C. Ramshaw, Lab Chip 1, 10 (2001)
4. M. Curcio, J. Roeraade, Anal. Chem. 75, 1 (2003)
5. J. Felbel, I. Bieber, J. Pieper, J.M. Köhler, Chem. Eng. J. 101, 333 (2004)
6. J. Felbel, A. Reichert, M. Kielpinski, M. Urban, T. Henkel, N. Häfner, M. Dürst, J. Weber, Chem. Eng. J. 135S, S298 (2008)
7. T. Fukuba, T. Yamamoto, T. Naganuma, T. Fujii, Chem. Eng. J. 101, 151 (2004)
8. A. Funfak, A. Brösing, M. Brand, J.M. Köhler, Lab. Chip. 7, 1132 (2007)
9. A. Grodrian, J. Metze, T. Henkel, K. Martin, M. Roth, J.M. Köhler, Biosens. Bioelectron. 19, 1421 (2004)
10. Z. Guttenberg, H. Müller, H. Habermüller, A. Geisbauer, J. Pipper, J. Felbel, M. Kielpinski, J. Scriba, A. Wixforth, Lab Chip 5, 308 (2005)
11. M. Halbach, K. Koschel, J. Gen. Virol. 42, 615 (1979)
12. T. Henkel, T. Bermig, M. Kielpinski, A. Grodrian, J. Metze, J.M. Köhler, Chem. Eng. J. 101, 439 (2004)
13. J.M. Köhler, T. Henkel, A. Grodrian, T. Kirner, M. Roth, K. Martin, J. Metze, Chem. Eng. J. 101, 201 (2004)
14. M.U. Kopp, A.J. Mello, A. Manz, Science 280, 1046 (1998)
15. K. Martin, T. Henkel, V. Baier, A. Grodrian, T. Schon, M. Roth, J. Michael Köhler, J. Metze, Lab Chip 3, 202 (2003)
16. T. Nisisako, T. Torii, T. Higuchi, Lab. Chip. 2, 24 (2002)
17. N. Park, S. Kim, J.H. Hahn, Anal. Chem. 75, 6029 (2003)
18. S. Poser, T. Schulz, U. Dillner, V. Baier, J.M. Köhler, D. Schimkat, G. Mayer, A. Siebert, Sens. Actuators 62, 672 (1997)
19. I. Schneegass, J.M. Köhler, Rev. Mol. Biotech. 82, 101 (2001)
20. H. Song, J.D. Tice, R.F. Ismagilov, Angew. Chem. Int. Ed. Engl. 42, 768 (2003)
21. T. Thorsen, R.W. Roberts, F.H. Arnold, S.R. Quake, Phys. Rev. Lett. 86, 4163 (2001)
22. P. Wilding, M.A. Shoffner, L.J. Kricka, Clin. Chem. 40, 1815 (1994)
23. A.T. Woolley, D. Hadley, P. Landre, A.J. deMello, R.A. Mathies, M.A. Northrup, Anal. Chem. 68, 4081 (1996)
24. C. Zhang, J. Xu, J. Wang, H. Wang, Anal. Lett. 40, 497 (2007)