Neonatal blood cultures: Effect of delayed entry into the blood culture machine and bacterial concentration on the time to positive growth in a simulated model

Journal of Paediatrics and Child Health

Volumn 45, Issue 4, 2009, Pages 210-214

  Jardine, Luke Anthony ^a,b   Sturgess, Barbara Ruth ^a   Inglis, Garry Donald Trevor ^a,b   Davies, Mark William ^a,b  

^a ROYAL BRISBANE AND WOMEN'S HOSPITAL   (Australia)

^b UNIVERSITY OF QUEENSLAND   (Australia)

Author keywords

Bacteremia; Bacteriological techniques; Blood cultures; Infant, newborn

Indexed keywords

ARTICLE; BACTERIAL GROWTH; BLOOD CULTURE; COLONY FORMING UNIT; CONTROLLED STUDY; ESCHERICHIA COLI; INOCULATION; NONHUMAN; PRIORITY JOURNAL; SIMULATION; STAPHYLOCOCCUS EPIDERMIDIS; STREPTOCOCCUS AGALACTIAE;

ANALYSIS OF VARIANCE; BACTERIOLOGICAL TECHNIQUES; ESCHERICHIA COLI; HUMANS; INFANT, NEWBORN; QUEENSLAND; SEPSIS; SPECIMEN HANDLING; STAPHYLOCOCCUS EPIDERMIDIS; STREPTOCOCCUS; TEMPERATURE; TIME FACTORS;

EID: 65349091479     PISSN: 10344810     EISSN: 14401754     Source Type: Journal    
DOI: 10.1111/j.1440-1754.2008.01455.x     Document Type: Article

References (22)

1. Stoll BJ, Holman RC, Schuchat A. Decline in sepsis-associated neonatal and infant deaths in the United States, 1979 through 1994. Pediatrics 1998 102 : e18.
2. Pazzi D, Klein J, Baker C. Bacterial sepsis and meningitis. In : Remington JS, ed. Infectious Diseases of the Fetus and Newborn Infant, 6th edn. Philadelphia, PA : Elsevier Saunders, 2006 248 95.
3. Tallur SS, Kasturi AV, Nadgir SD, Krishna BV. Clinico-bacteriological study of neonatal septicemia in Hubli. Indian J. Pediatr. 2000 67 : 169 74.
4. Kumar Y, Qunibi M, Neal TJ, Yoxall CW. Time to positivity of neonatal blood cultures. Arch. Dis. Child 2001 85 : F182 6.
5. Kurlat I, Stoll BJ, McGowan JE, Jr. Time to positivity for detection of bacteremia in neonates. J. Clin. Microbiol. 1989 27 : 1068 71.
6. Garcia-Prats JA, Cooper TR, Schneider VF, Stager CE, Hansen TN. Rapid detection of microorganisms in blood cultures of newborn infants utilizing an automated blood culture system. Pediatrics 2000 105 : 523 7.
7. Escobar GJ, Zukin T, Usatin MS et al. Early discontinuation of antibiotic treatment in newborns admitted to rule out sepsis: a decision rule. Pediatr. Infect. Dis. J. 1994 13 : 860 6.
8. Hurst MK, Yoder BA. Detection of bacteremia in young infants: is 48 hours adequate? Pediatr. Infect. Dis. J. 1995 14 : 711 13.
9. Pauli I Jr., Shekhawat P, Kehl S, Sasidharan P. Early detection of bacteremia in the neonatal intensive care unit using the new BACTEC system. J. Perinatol. 1999 19 : 127 31.
10. Rowley AH, Wald ER. Incubation period necessary to detect bacteremia in neonates. Pediatr. Infect. Dis. J. 1986 5 : 590 1.
11. Haimi-Cohen Y, Vellozzi EM, Rubin LG. Initial concentration of Staphylococcus epidermidis in simulated pediatric blood cultures correlates with time to positive results with the automated, continuously monitored BACTEC blood culture system. J. Clin. Microbiol. 2002 40 : 898 901.
12. Li J, Plorde JJ, Carlson LG. Effects of volume and periodicity on blood cultures. J. Clin. Microbiol. 1994 32 : 2829 31.
13. Plorde JJ, Tenover FC, Carlson LG. Specimen volume versus yield in the BACTEC blood culture system. J. Clin. Microbiol. 1985 22 : 292 5.
14. Ilstrup DM, Washington I, John A. The importance of volume of blood cultured in the detection of bacteremia and fungemia. Diagn. Microbiol. Infect. Dis. 1983 1 : 107 10.
15. Sandven P, Hoiby EA. The importance of blood volume cultured on detection of bacteraemia. Acta Pathol Microbiol Scand [B] 1981 89 : 149 52.
16. Tenney JH, Reller LB, Mirrett S, Wang WL, Weinstein MP. Controlled evaluation of the volume of blood cultured in detection of bacteremia and fungemia. J. Clin. Microbiol. 1982 15 (4 558 61.
17. Mermel LA, Maki DG. Detection of bacteremia in adults: consequences of culturing an inadequate volume of blood. Ann. Intern. Med. 1993 119 : 270 2.
18. Neal PR, Kleiman MB, Reynolds JK, Allen SD, Lemons JA, Yu PL. Volume of blood submitted for culture from neonates. J. Clin. Microbiol. 1986 24 (3 353 6.
19. Dietzman DE, Fischer GW, Schoenknecht FD. Neonatal Escherichia coli septicemia - bacterial counts in blood. J. Pediatr. 1974 85 : 128 30.
20. Schelonka RL, Chai MK, Yoder BA, Hensley D, Brockett RM, Ascher DP. Volume of blood required to detect common neonatal pathogens. J. Pediatr. 1996 129 : 275 8.
21. Jardine LA, Davies MW, Faoagali J. Incubation time required for neonatal blood cultures to become positive. J. Paediatr. Child Health 2006 42 : 797 802.
22. Haimi-Cohen Y, Shafinoori S, Tucci V, Rubin LG. Use of incubation time to detection in BACTEC 9240 to distinguish coagulase-negative staphylococcal contamination from infection in pediatric blood cultures. Pediatr. Infect. Dis. J. 2003 22 : 968 74.