Micro-CT analysis with multiple thresholds allows detection of bone formation and resorption during ultrasound-treated fracture healing

Journal of Orthopaedic Research

Volumn 27, Issue 5, 2009, Pages 673-679

  Freeman, Theresa A ^a   Patel, Payal ^a   Parvizi, Javad ^a   Antoci Jr , Valentin ^a   Shapiro, Irving M ^a  

^a THOMAS JEFFERSON UNIVERSITY   (United States)

Author keywords

Bone fracture; Fracture callus; Micro CT analysis; Resorption; Ultrasound

Indexed keywords

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BONE REMODELING; CONTROLLED STUDY; CORTICAL BONE; FRACTURE HEALING; IN VIVO STUDY; MALE; MICRO-COMPUTED TOMOGRAPHY; NONHUMAN; OSSIFICATION; OSTEOLYSIS; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; RAT; TREATMENT OUTCOME; ULTRASOUND THERAPY; ANIMAL; BONE DEVELOPMENT; BONE MINERALIZATION; CALLUS; ECHOGRAPHY; FEMUR; INJURY; METHODOLOGY; PHYSIOLOGY; THREE DIMENSIONAL IMAGING; WISTAR RAT;

ANIMALS; BONE RESORPTION; BONY CALLUS; CALCIFICATION, PHYSIOLOGIC; FEMUR; FRACTURE HEALING; IMAGING, THREE-DIMENSIONAL; MALE; MODELS, ANIMAL; OSTEOGENESIS; RATS; RATS, WISTAR; ULTRASONIC THERAPY; X-RAY MICROTOMOGRAPHY;

EID: 65249141042     PISSN: 07360266     EISSN: None     Source Type: Journal    
DOI: 10.1002/jor.20771     Document Type: Article

References (20)

1. Bolander ME. 1992. Regulation of fracture repair by growth factors. Proc Soc Exp Biol Med 200:165-170.
2. Simmons DJ. 1985. Fracture healing perspectives Clin Orthop Relat Res. 200:100-113.
3. Feldkamp LA, Goldstein SA, Parfitt AM, et al. 1989. Direct examination of three-dimensional bone architecture in vitro by computed tomography. J Bone Miner Res 4:3-11.
4. Andreassen TT, Ejersted C, Oxlund H. 1999. Intermittent parathyroid hormone treatment increases callus formation and mechanical strength of healing rat fractures. J Bone Miner Res 14:960-968.
5. BaxBA, Wozney JM, Ashhurst DE. 1999. Bone morphogenetic protein-2 increases the rate of callus formation of the rabbit tibia. Calcif Tissue Int 65:83-89.
6. Walsh WR, Sherman P, Howlett CR, et al. 1997. Fracture healing in a rat osteopenia model Clin Orthop Relat Res. 342:218-227.
7. Heckman JD, Ryaby JP, McCabe J, et al. 1994. Acceleration of tibial fracture-healing by non-invasive, low intensity pulsed ultrasound. J Bone Joint Surg 76A:26-34.
8. Leung KS, Lee WS, Tsui HF, et al. 2004. Complex tibial fracture outcomes following treatment with low-intensity pulsed ultrasound. Ultrasound Med Biol 30:389-395.
9. Pilla AA, Mont MA, Nasser PR, et al. 1990. Non-invasive low- intensity pulsed ultrasound accelerates bone healing in the rabbit. J Orthopaed Trauma 4:246-253.
10. Ebisawa K, Hata K-I, Okada K, et al. 2004. Ultrasound enhances transforming growth factor B-mediated chondrocyte differentiation of mesenchymal stem cells. Tissue Eng 10:921 -929.
11. Leung KS, Cheung WH, Zhang C, et al. 2004. Low intensity pulsed ultrasound stimulates osteogenic activity of human periosteal cells. Clin Orthop Relat Res 418:253-259.
12. Azuma Y, Ito M, Harada Y, et al. 2001. Low-intensity pulsed ultrasound accelerates rat femoral fracture healing by acting on the various cellular reactions in the fracture callus. J Bone Miner Res 16:671-680.
13. Parvizi J, Parpura V, Greenleaf JF, et al. 2002. Calcium signalling is required for ultrasound-stimulated aggrecan synthesis by rat chondrocytes. J Orthop Res 20:51 -57.
14. Bonnarens F, Einhorn TA. 1984. Production of a standard closed fracture in laboratory animal bone. J Biomech 4:155-158.
15. Yang KH, Parvizi J, Wang SJ, et al. 1996. Exposure to low- intensity ultrasound increases aggrecan gene expression in a rat femoral fracture model. J Orthop Res 14:802-809.
16. Snedecor GW, Cochran WG. 1967. Statistical methods. Ames, IA: The Iowa State University Press.
17. Gerstenfeld LC, Alkhiary YM, Krall EA, et al. 2006. Three dimensional reconstruction of fracture callus morphogenesis. J Histochem Cytochem 54:1215-1228.
18. Pritchett DD. 1992. Longitudinal growth and growth-plate activity in the lower extremity. Clin Orthop Relat Res 275:274-279.
19. Kuhn JL, DeLacey JH, Leenellett EE. 1996. Relationship between bone growth rate and hypertrophic chondrocyte volume in New Zealand white rabbits of varying ages. J Orthop Res 14:706-711.
20. Farnum CE, Lee AO, O'Hara K, et al. 2003. Effect of short- term fasting on bone elongation rates: an analysis of catch-up growth in young male rats. Pedatr Res 53:33-41.