Ex vivo and in vivo second-harmonic-generation imaging of dermal collagen fiber in skin: Comparison of imaging characteristics between mode-locked Cr:forsterite and Ti:Sapphire lasers

Applied Optics

Volumn 48, Issue 10, 2009, Pages

  Yasui, Takeshi ^a   Takahashi, Yu ^a   Ito, Masahiro ^a   Fukushima, Shuichiro ^a   Araki, Tsutomu ^a  

^a OSAKA UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

COLLAGEN; LOCKS (FASTENERS); MICROSCOPES; MODE-LOCKED FIBER LASERS; NONLINEAR OPTICS; OLIVINE; SAPPHIRE; SOLID STATE LASERS;

CR : FORSTERITE LASER; IMAGING CHARACTERISTICS; IN-VIVO IMAGING; MODE-LOCKED TI: SAPPHIRE LASERS; SCATTERING AND ABSORPTION; SECOND HARMONIC GENERATION MICROSCOPIES (SHG); SECOND HARMONICS; TI: SAPPHIRE LASER;

HARMONIC GENERATION;

ALUMINUM OXIDE; CHROMIUM; COLLAGEN; MAGNESIUM ORTHOSILICATE; SILICON DERIVATIVE;

ADULT; ANIMAL; ARTICLE; COMPARATIVE STUDY; CONFOCAL MICROSCOPY; DERMIS; EVALUATION; HUMAN; INSTRUMENTATION; LASER; MALE; METABOLISM; METHODOLOGY; MIDDLE AGED;

ADULT; ALUMINUM OXIDE; ANIMALS; CHROMIUM; COLLAGEN; DERMIS; HUMANS; LASERS; MALE; MICROSCOPY, CONFOCAL; MIDDLE AGED; SILICON COMPOUNDS;

EID: 65249114771     PISSN: 1559128X     EISSN: 15394522     Source Type: Journal    
DOI: 10.1364/AO.48.000D88     Document Type: Article

References (32)

1. S. Roth and I. Freund, "Optical second-harmonic scattering in rat-tail tendon," Biopolymers 20, 1271-1290 (1981).
2. G. Cox, E. Kable, A. Jones, I. Fraser, F. Manconi, and M. D. Gorrell, "3-dimensional imaging of collagen using second harmonic generation," J. Structural Biol. 141, 53-62 (2003).
3. K Konig and I. Riemann, "High-resolution multiphoton tomography of human skin with subcellular spatial resolution and picosecond time resolution," J. Biomed. Opt. 8, 432-439 (2003).
4. J. A. Palero, H. S. de Bruijn, A. van der Ploeg-van den Heuvel, H. J. C. M. Sterenborg, and H. C. Gerritsen, "In vivo nonlinear spectral imaging in mouse skin," Opt. Express 14, 4395-4402 (2006).
5. T. Yasui, Y. Tohno, and T. Araki, "Determination of collagen fiber orientation in human tissue by polarization measurement of molecular second-harmonic-generation light," Appl. Opt. 43, 2861-2867 (2004).
6. T. Yasui, Y. Tohno, and T. Araki, "Characterization of collagen orientation in human dermis by two-dimensional second-harmonic-generation polarimetry," J. Biomed. Opt. 9, 259-264 (2004).
7. T. Yasui, K. Sasaki, Y. Tohno, and T. Araki, "Tomographic imaging of collagen fiber orientation in human tissue using depth-resolved polarimetry of second-harmonic-generation light," Opt. Quantum Electron. 37, 1397-1408 (2005).
8. A. K. Dunn, V. P. Wallace, M. Coleno, M. W. Berns, and B. J. Tromberg, "Influence of optical properties on two-photon fluorescence imaging in turbid samples," Appl. Opt. 39, 1194-1201 (2000).
9. C.-Y. Dong, K. Koenig, and P. So, "Characterizing point spread functions of two-photon fluorescence microscopy in turbid medium," J. Biomed. Opt. 8, 450-459 (2003).
10. U. K. Tirlapur, K. König, C. Peuckert, R. Krieg, and J. J. Halbhuber, "Femtosecond near infrared laser pulses elicit generation of reactive oxygen species in mammalian cells leading to apoptosis-like death," Exp. Cell Research 263, 88-97 (2001).
11. K König, P. T. C. So, W. W. Mantulin, and E. Gratton, "Cellular response to near-infrared femtosecond laser pulses in two photon microscope," Opt. Lett. 22, 135-136 (1997).
12. P. C. Cheng, S. J. Pan, A. Shih, K-S. Kim, W. S. Liou, and M. S. Park, "Highly efficient upconverters for multiphoton fluorescence microscopy," J. Microsc. 189, 199-212 (1998).
13. R. R. Anderson and J. A. Parish, "The optics of human skin," J. Invest. Dermatol. 77, 13-19 (1981).
14. S.-W. Chu, S.-Y. Chen, T.-H. Tsai, T.-M. Liu, C.-Y. Lin, H.-J. Tsai, and C.-K Sun, "In vivo developmental biology study using noninvasive multi-harmonic generation microscopy," Opt. Express 11, 3093-3099 (2003).
15. S.-P. Tai, W.-J. Lee, D.-B. Shieh, P.-C. Wu, H.-Y. Huang, C.-H. Yu, and C.-K Sun, "In vivo optical biopsy of hamster oral cavity with epi-third-harmonic-generation microscopy," Opt. Express 14, 6178-6187 (2006).
16. S.-P. Tai, T.-H. Tsai, W.-J. Lee, D.-B. Shieh, Y.-H. Liao, H.-Y. Huang, K. Zhang, H.-L. Liu, and C.-K Sun, "Optical biopsy of fixed human skin with backward-collected optical harmonics signals," Opt. Express 13, 8231-8242 (2005).
17. S.-Y. Chen, C.-Y. S. Hsu, and C.-K. Sun, "Epi-third and second harmonic generation microscopic imaging of abnormal enamel," Opt. Express 16, 11670-11679 (2008).
18. I.-H. Chen, S.-W. Chu, C.-K Sun, P.-C. Cheng, and B.-L. Lin, "Wavelength dependent damage in biological multi-photon confocal microscopy: a microspectroscopic comparison between femtosecond Ti:sapphire and Cr:forsterite laser sources," Opt. Quantum. Electron 34, 1251-1266 (2002).
19. T.-H. Tsai, S.-P. Tai, W.-J. Lee, H.-Y. Huang, Y.-H. Liao, and C.-K. Sun, "Optical signal degradation study in fixed human skin using confocal microscopy and higher-harmonic optical microscopy," Opt. Express 14, 749-758 (2006).
20. G. J. Teamey, M. E. Brezinski, J. F. Southern, B. E. Bouma, M. R. Hee, and J. G. Fujimoto, "Determination of the refractive index of highly scattering human tissue by optical coherence tomography," Opt. Lett. 20, 2258-2260 (1995).
21. L. Sherman, J. Y. Ye, O. Albert, and T. B. Norris, "Adaptive correction of depth-induced aberrations in multiphoton scanning microscopy using a deformable mirror," J. Microsc. 206, 65-71 (2002).
22. P. Marsh, D. Burns, and J. Girkin, "Practical implementation of adaptive optics in multiphoton microscopy," Opt. Express 11, 1123-1130 (2003).
23. Y. Jiang, I. Tomov, Y. Wang, and Z. Chen, "Second-harmonic optical coherence tomography," Opt. Lett. 29, 1090-1092 (2004).
24. J. Su, I. V. Tomov, Y. Jiang, and Z. Chen, "High-resolution frequency-domain second-harmonic optical coherence tomography," Appl. Opt. 46, 1770-1775 (2007).
25. Y. Guo, P. P. Ho, H. Savage, D. Harris, P. Sacks, S. Schantz, F. Liu, N. Zhadin, and R. R. Alfano, "Second-harmonic tomography of tissues," Opt. Lett. 22, 1323-1325 (1997).
26. F. Fischer, B. Volkmer, S. Puschmann, R. Greinert, W. Breitbart, J. Kiefer, and R. Wepf, "Risk estimation of skin damage due to ultrashort pulsed, focused near-infrared laser irradiation at 800 nm," J. Biomed. Opt. 13, 041320 (2008).
27. S.-Y. Chen and C.-K Sun, "In vivo imaging of human skin using harmonic generation microscopy," in Abstracts. Focus on Microscopy 2008 (2008), p. 59.
28. L. H. Kligman, E. Schwartz, A. N. Sapadin, and A. M. Kligman, "Collagen loss in photoaged human skin is overestimated by histochemistry," Photodermatol. Photoimmunol. Photomed. 16, 224-228 (2000).
29. S.-J. Lin, R.-Jr. Wu, H.-Y. Tan, W. Lo, W.-C. Lin, T.-H. Young, C.-J. Hsu, J.-S. Chen, S.-H. Jee, and C.-Y. Dong, "Evaluating cutaneous photoaging by use of multiphoton fluorescence and second-harmonic generation microscopy," Opt. Lett. 30, 2275-2277 (2005).
30. M. J. Koehler, K König, P. Eisner, R. Bückle, and M. Kaatz, "In vivo assessment of human skin aging by multiphoton laser scanning tomography," Opt. Lett. 31, 2879-2881 (2006).
31. T. Yasui, Y. Takahashi, T. Araki, Y. Ogura, Y. Matsunaga, and T. Kuwahara, "Observation of photoaged, dermal collagen fiber using polarization-resolved second-harmonic-generation microscopy," J. Invest. Dermatol. 128, S40 (2008).
32. T. Yasui, Y. Takahashi, S. Fukushima, Y. Ogura, T. Yamashita, T. Kuwahara, T. Hirao, and T. Araki, "Observation of dermal collagen fiber in wrinkled skin using polarization-resolved second-harmonic-generation microscopy," Opt. Express 17, 912-923 (2009).1234