Femtosecond near-infrared laser pulses elicit generation of reactive oxygen species in mammalian cells leading to apoptosis-like death

Experimental Cell Research

Volumn 263, Issue 1, 2001, Pages 88-97

  Tirlapur, U K ^a   Konig K ^a   Peuckert, C ^a   Krieg, R ^a   Halbhuber, K ^a  

^a FRIEDRICH SCHILLER UNIVERSITY JENA   (Germany)

Author keywords

Apoptosis; DNA strand breaks; Femtosecond laser; Near infrared; Nuclear fragmentation; Oxidative stress; ROS; Two photon microscopy

Indexed keywords

4',6 DIAMIDINO 2 PHENYLINDOLE; ADENOSINE TRIPHOSPHATASE (CALCIUM); DIAMINOBENZIDINE; DNA FRAGMENT; FLUORESCENT DYE; HYDROGEN PEROXIDE; HYDROXYL RADICAL; MEMBRANE LIPID; MEMBRANE PROTEIN; NICKEL; PROPIDIUM IODIDE; REACTIVE OXYGEN METABOLITE;

ANIMAL CELL; APOPTOSIS; ARTICLE; CELL DIVISION; CELL MEMBRANE; CELL NUCLEUS; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; CYTOCHEMISTRY; DNA STRAND BREAKAGE; EPITHELIUM CELL; FLUORESCENCE MICROSCOPY; LASER; LIPID PEROXIDATION; MEMBRANE DAMAGE; NICK END LABELING; NONHUMAN; OXIDATIVE PHOSPHORYLATION; OXIDATIVE STRESS; PEROXISOME; PH; PRIORITY JOURNAL;

MACROPUS SP.; MAMMALIA; POTOROIDAE;

EID: 0035242368     PISSN: 00144827     EISSN: None     Source Type: Journal    
DOI: 10.1006/excr.2000.5082     Document Type: Article

References (56)

1. Two-photon laser scanning fluorescence microscope
2. Prospects of non-linear microscopy in the next decade: An overview
3. Introduction to two-photon microscopy
4. Multiphoton microscopy in life sciences
5. Multiphoton excitation provides optical sections from deeper within scattering specimens than confocal imaging
6. Long-term two-photon fluorescence imaging of mammalian embryos without compromising viability
7. Multiphoton fluorescence excitation - New spectral windows for biological non-linear microscopy
8. Optical properties of human brain
9. Near-infrared femtosecond laser pulses as a novel non-invasive means for dye-permeation and 3D imaging of localised dye-coupling in the Arabidopsis root meristem
10. Three-photon excitation fluorescence imaging of biological specimens using an all-solid-state laser
11. Multiphoton excitation microscopy to study biosystems
12. Imaging living cells and tissues by two-photon excitation microscopy
13. Two-photon deep tissue ex vivo imaging of mouse dermal and subcutaneous structures
14. Two-photon imaging in living brain slices
15. An evaluation of two-photon excitation verses confocal and digital deconvolution fluorescence microscopy imaging in Xenopus morphogenesis
16. 2+ fluorescence imaging with pico- and femtosecond two-photon excitation: Signal and photodamage
17. Non-invasive two-photon-excitation imaging of tryptophan distribution in highly scattering biological tissues
18. Photobleaching of fluorescent dyes under conditions used for single-molecule detection: Evidence of two-step photolysis
19. Two-photon excited lifetime imaging of autofluorescence in cells during UVA and NIR photostress
20. Cellular response to near-infrared femtosecond laser pulses in two-photon microscopes
21. Pulse-length dependence of cellular response to intense near-infrared laser pulses in multiphoton microscopes
22. Multifocal multiphoton microscopy: A fast and efficient tool for 3-D fluorescence imaging
23. Ultrastructure and reproduction behaviour of single CHO-K1 cells exposed to near infrared femtosecond laser pulses
24. Translocation of active mitochondria during hamster preimplantation embryo development studied by confocal laser scanning microscopy
25. The Schrödinger's cat quandary in cell biology: Integration of live functional analysis with measurements of fixed cells in analysis of apoptosis
26. Mode of injury and death after hydrogen peroxide exposure in cultured Oligodendroglia cells
27. Reflectance enzyme histochemistry (REH): Visualisation of cerium-based DAB primary reaction products of phosphatases and oxidases in cryostst sections by confocal laser scanning microscopy
28. Laser scanning microscopy in enzyme histochemistry: Visualisation of cerium-based and DAB-based primary reaction products of phosphatases, oxidases and peroxidases by reflectance and transmission laser scanning microscopy
29. Current cytochemical techniques for the investigation of peroxisomes: A review
30. Cytochemical demonstration of expression and distribution of human cathepsin in HEK 23 cells
31. Two-photon molecular excitation in laser scanning microscopy
32. Comparison of two-photon excitation laser scanning microscopy with UV-confocal laser scanning microscopy in three-dimensional calcium imaging using the fluorescence indicator Indo-1
33. Effects of the environment on the fluorescence of aromatic compounds in solution
34. Tailoring luminescent compounds and photosensitizers: Cage-type metal complexes
35. Selective oxidations catalysed by peroxidases
36. 2 in mammalian cells
37. The interaction of UVA radiation with cultured cells
38. UV-B mediated damage in the photosynthetic flagellate, Euglena gracilis, studied by image analysis
39. Effects of increased solar ultraviolet radiation on terrestrial ecosystems
40. Role of hydrogen peroxide in the cytotoxic effects of UVA/B radiation on mammalian cells
41. Evidence that hydrogen peroxide generated by 365-nm UVA radiation is not important in mammalian cell killing
42. Gap junction channels: New roles in disease
43. Alteration of cell membrane by stress waves in vitro
44. Oxidative stress-mediated apoptosis of hepatocytes exposed to acute ethanol intoxication
45. 2 exposure and the inhibitory effect of U78517F
46. Oxidant stress activates a non-selective cation channel responsible for membrane depolarization in calf vascular endothelial cells
47. Near-visible ultraviolet light induces a novel ubiquitous calcium-permeable cation current in mammalian cell lines
48. Programmed cell death in plants
49. Cell death in health and disease: The biology and regulation of apoptosis
50. Oxidative stress as a mediator of apoptosis
51. Two types of the enlargement of mitochondria related to apoptosis: Simple swelling and the formation of megamitochondria
52. DNA strand breaks occurring during apoptosis: Their early in situ detection by the terminal deoxynucleotidyl transferase and nick translation assays and prevention by serine protease inhibitors
53. Multiparametric staining to identify apoptotic human cells
54. Multiphoton near-infrared femtosecond laser pulse-induce DNA damage with and without the photosensitizer proflavine
55. Nuclear changes in apoptosis