Qualitative and quantitative detection of Chlamydophila pneumoniae DNA in cerebrospinal fluid from multiple sclerosis patients and controls

PLoS ONE

Volumn 4, Issue 4, 2009, Pages

  Tang, Yi Wei ^a   Sriram, Subramaniam ^a   Li, Haijing ^a   Yao, Song Yi ^a   Meng, Shufang ^a   Mitchell, William M ^a   Stratton, Charles W ^a  

^a VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA;

ARTICLE; CEREBROSPINAL FLUID LEVEL; CHLAMYDIAL PNEUMONIA; CHLAMYDOPHILA PNEUMONIAE; CONTROLLED STUDY; DIAGNOSTIC VALUE; DNA DETERMINATION; ENZYME IMMUNOASSAY; HUMAN; INTERMETHOD COMPARISON; MAJOR CLINICAL STUDY; MICROTITER PLATE ASSAY; MULTIPLE SCLEROSIS; QUALITATIVE ANALYSIS; QUANTITATIVE DIAGNOSIS; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; VALIDATION STUDY; CEREBROSPINAL FLUID; CHLAMYDOPHILA INFECTION; ENZYME LINKED IMMUNOSORBENT ASSAY; EVALUATION; GENETICS; INSTRUMENTATION; METHODOLOGY; MICROBIOLOGY; PATHOPHYSIOLOGY; POLYMERASE CHAIN REACTION;

CHLAMYDOPHILA PNEUMONIAE;

CHLAMYDOPHILA INFECTIONS; CHLAMYDOPHILA PNEUMONIAE; DNA, BACTERIAL; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; MULTIPLE SCLEROSIS; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY;

EID: 65249100695     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0005200     Document Type: Article

References (51)

1. Grayston JT, Campbell LA, Kuo CC, Mordhorst CH, P. Saikku P, et al. (1990) A new respiratory tract pathogen: Chlamydia pneumoniae strain TWAR. J Infect Dis 161: 618-625.
2. Stratton CW, Wheldon DB (2006) Multiple sclerosis: an infectious syndrome involving Chlamydophila pneumoniae. Trends Microbiol 14: 474-479.
3. Fainardi E, Castellazzi M, Seraceni S, Granieri E, Contini C (2008) Under the microscope: focus on Chlamydia pneumoniae infection and multiple sclerosis. Curr Neurovasc Res 5: 60-70.
4. Boman J, Allard A, Persson K, Lundborg M, Juto P, et al. (1997) Rapid diagnosis of respiratory Chlamdyia pneumoniae infection by nested touchdown polymerase chain reaction compared with culture and antigen detection by EIA. J Infect Dis 175: 1523-1526.
5. Boman J, Gaydos CA, Quinn TC (1999) Molecular diagnosis of Chlamydia pneumoniae infection. J Clin Microbiol 37: 3791-3799.
6. Yan Y, Silvennoinen-Kassinen S, Leinonen M, Saikku P (2005) Methodological aspects affecting the infectivity of Chlamydia pneumoniae in cell cultures in vitro. J Microbiol Methods 61: 127-130.
7. Mahony JB, Chong S, Coombes BK, Smieja M, Petrich A (2000) Analytical sensitivity, reproducibility of results, and clinical performance of five PCR assays for detecting Chlamydia pneumoniae DNA in peripheral blood mononuclear cells. J Clin Microbiol 38: 2622-2627.
8. Fukano H (2004) Comparison of five PCR assays for detecting Chlamydia pneumoniae DNA. Microbiol Immunol 48: 441-448.
9. Dowell SF, Peeling RW, Boman J, Carlone GM, Fields BS, et al. (2001) Standardizing Chlamydia pneumoniae assays: recommendations from the Centers for Diseases Control and Prevention (USA) and the Laboratory Centre for Disease Control (Canada). Clin Infect Dis 33: 492-503.
10. Yamamoto Y (2002) PCR in diagnosis of infection: detection of bacteria in cerebrospinal fluids. Clin Diagn Lab Immunol 9: 508-514.
11. Sriram S, Stratton CW, Yao S, Tharp A, Ding L, et al. (1999) Chlamydia pneumoniae infection of the central nervous system in multiple sclerosis. Ann Neurol 46: 6-14.
12. Ikejima H, Haranaga S, Takemura H, Kamo T, Takahashi Y, et al. (2001) PCR-based method for isolation and detection of Chlamdyia pneumoniae DNA in cerebrospinal fluids. Clin Diagn Lab Immunol 8: 499-502.
13. Black CM, Fields PI, Messmer TO, Berdal BP (1994) Detection of Chlamydia pneumoniae in clinical specimens by polymerase chain reaction using nested primers. Eur J Clin Microbiol Infect Dis 13: 752-756.
14. Smalling TW, Sefers SE, Li HJ, Tang YW (2002) Molecular approaches to detecting herpes simplex and enteroviruses in the central nervous system. J Clin Microbiol 40: 2317-2322.
15. Tang YW, Rys PN, Rutledge BJ, Mitchell PS, Smith TF, et al. (1998) Comparative evaluation of colometric microtiter plate systems for detection of herpes simplex virus in cerebrospinal fluid. J Clin Microbiol 36: 2714-2717.
16. Ng SY, Gunning P, Eddy R, Ponte P, Leavitt J, et al. (1985) Evolution of the functional human beta-actin gene and its multi-pseudogene family: conservation of noncoding regions and chromosomal dispersion of pseudogenes. Mol Cell Biol 5: 2720-2732.
17. Melgosa P, Kuo CC, Campbell LA (1991) Sequence analysis of the major outer membrane protein gene of Chlamydia pneumoniae. Infect Immun 59: 2195-2199.
18. Watson MW, Lambden PR, Clarke IN (1991) Genetic diversity and identification of human infection by amplification of the chlamydial 60-kilodalton cysteine-rich outer membrane protein gene. J Clin Microbiol 29: 1188-1193.
19. Tang YW, Mitchell PS, Espy MJ, Smith TF, Persing DH (1999) Molecular diagnosis of herpes simplex virus infections in the central nervous system. J Clin Microbiol 37: 2127-2136.
20. Holland PM, Abramson RD, Watson R, Gelfand DH (1991) Detection of specific polymerase chain reaction product by utilizing the 5′-3′ exonuclease activity of Thermus aquaticus DNA polymerase. Proc Natl Acad Sci USA 88: 7276-7280.
21. Heid CA, Stevens J, Livak KJ, Williams PM (1996) Real time quantitative PCR. Genome Res 6: 986-994.
22. Khan MA, Potter CW (1996) The nPCR detection of Chlamydia pneumoniae and Chlamydia trachomatis in children hospitalized for bronchiolitis. J Infect 33: 173-175.
23. Sriram S, Yao SY, Stratton C, Calabresi P, Mitchell WM, et al. (2002) Comparative study of the presence of Chlamydia pneumoniae in cerebral spinal fluid from clinically definite and monosymptomatic multiple sclerosis. Clin Diagn Lab Immunol 9: 1332-1337.
24. Sriram S, Mitchell W, Stratton C (1998) Multiple sclerosis associated with Chlamydia pneumoniae infection of the CNS. Neurology 50: 571-572.
25. Layh-Schmitt G, Bendi C, Hildt U, Dong-Si T, Juttler E, et al. (2000) Evidence for infection with Chlamydia pneumoniae in a subgroup of patients with multiple sclerosis. Ann Neurol 47: 652-655.
26. Sotgiu S, Piana A, Pugliatti M, Sotgiu A, Deiana GA, et al. (2001) Chlamydia pneumoniae in the cerebrospinal fluid of patients with multiple sclerosis and neurological controls. Mult Scler 7: 371-374.
27. Gieffers J, Pohl D, Treib J, Dittmann R, Stephan C, et al. (2001) Presence of Chlamydia pneumoniae DNA in the cerebral spinal fluid is a common phenomenon in a variety of neurological diseases and not restricted to multiple sclerosis. Ann Neurol 49: 585-589.
28. Hao Q, Miyashita N, Wang H-Y, Matsushima T, Saida T (2002) Chlamydia pneumoniae infection associated with enhanced MRI spinal lesions in multiple sclerosis. Mult Scler 8: 436-440.
29. Grimaldi LM, Pincherle A, Martinelli-Boneschi F, Fillippi M, Patti F, et al. (2003) An MRI study of Chlamydia pneumoniae infection in Italian multiple sclerosis patients. Mult Scler 9: 467-471.
30. Contini C, Cultrera R, Seraceni S, Castellazzi M, Granieri E, et al. (2004) Cerebrospinal fluid molecular demonstration of Chlamydia pneumoniae DNA is associated to clinical and brain magnetic resonance imaging activity in a subset of patients with relapsing-remitting multiple sclerosis. Mult Scler 10: 360-369.
31. Dong-Si T, Weber J, Liu YB, Buhmann C, Bauer H, et al. (2004) Increased prevalence of and gene transcription by Chlamydia pneumoniae in cerebrospinal fluid of patients with relapsing-remitting multiple sclerosis. J Neurol 251: 542-547.
32. Contini C, Seraceni S, Castellazzi M, Granieri E, Fainaardi E (2008) Chlamydophila pneumoniae DNA and mRNA transcript levels in peripheral blood mononuclear cells and cerebrospinal fluid of patients with multiple sclerosis. Neurosci Res 62: 58-61.
33. Boman J, Roblin PM, Sundstrom P, Sandstrom M, Hammerschlag MR (2000) Failure to detect Chlamydia pneumoniae in the central nervous system of patients with MS. Neurology 54: 265.
34. Hammerschlag MR, Ke Z, Lu F, Roblin P, Boman J, Kalman B (2000) Is Chlamydia pneumoniae present in brain lesions of patients with multiple sclerosis? J Clin Microbiol 38: 4274-4276.
35. Morre SA, De Groot CJ, Killestein J, Meijer CJ, Polman CH, et al. (2000) Is Chlamydia pneumoniae present in the central nervous system of multiple sclerosis patients? Ann Neurol 48: 399.
36. Pucci E, Taus C, Cartechini E, Morelli M, Giuliani G, et al. (2000) Lack of Chlamydia infection of the central nervous system in multiple sclerosis. Ann Neurol 48: 399-400.
37. Saiz A, Marcos MA, Graus F, Vidal J, Jimenez de Anta MT (2001) No evidence of CNS infection with Chlamydia pneumoniae in patients with multiple sclerosis. J Neurol 248: 617-618.
38. Derfuss T, Gurkov R, Then Bergh F, Goebels N, Hartmann M, et al. (2001) Intrathecal antibody production against Chlamydia pneumoniae in multiple sclerosis is part of a polyspecific immune response. Brain 124: 1325-1335.
39. Numazaki K, Chibar S (2002) Failure to detect Chlamydia pneumoniae in the central nervous system of patients with MS. Neurology 57: 746.
40. Furrows SJ, Hartley JC, Bell J, Silver N, Losseff N, et al. (2004) Chlamydophila pneumoniae infection of the central nervous system in patients with multiple sclerosis. J Neruol Neurosurg Psychiatry 75: 152-154.
41. Kaufman M, Gaydos CA, Sriram S, Boman J, Tondella ML, et al. (2002) Is Chlamydia pneumoniae found in spinal fluid samples from multiple sclerosis patients? Conflicting results. Mult Scler 8: 289-294.
42. Fredricks DN, Relman DA (1999) Application of polymerase chain reaction to the diagnosis of infectious diseases. Clin Infect Dis 29: 475-486.
43. Boman J, Hammerschlag MR (2002) Chlamydia pneumoniae and atherosclerosis: critical assessment of diagnostic methods and relevance to treatment studies. Clin Microbiol Rev 15: 1-20.
44. Apfalter P, Blasi R, Boman J, Gaydos CA, Kundi M, et al. (2001) Multicenter comparison trial of DNA extraction methods and PCR assays for detection of Chlamydia pneumoniae in endarterectomy specimens. J Clin Microbiol 39: 519-524.
45. Smieja M, Mahony JB, Goldsmith CH, Chong S, Petrich A, et al. (2001) Replicate PCR testing and probit analysis for detection and quantitation of Chlamdyia pneumoniae in clinical specimens. J Clin Microbiol 39: 1796-1801.
46. Inman-Bamber J, Wan C, Gardam T, Vohra R, van Daal A, et al. (2002) Novel PCR-EIA method for the detection of Chlamydia pneumoniae in respiratory specimens. Mol Cell Probes 16: 57-61.
47. Flamand L, Gravel A, Boutolleau D, Alvarez-Lafuente R, et al. (2008) Multicenter comparison of PCR assays for detection of human herpesvirus 6 DNA in serum. J Clin Microiol 46: 2700-2706.
48. Kuoppa Y, Bowman J, Scott L, Kumlin U, Eriksson I, et al. (2002) Quantitative detection of respiratory Chlamyidia pneumoniae infection by real-time PCR. J Clin Microbiol 40: 2273-2274.
49. Tondella ML, Talkington DF, Holloway BP, Dowell SF, Cowley K, et al. (2002) Development and evaluation of real-time PCR fluorescence assays for detection of Chlamydia pneumoniae. J Clin Microbiol 40: 575-583.
50. Sriram S, Ljunggren-Rose A, Yao S-Y, Whetsell WO Jr (2005) Detection of chlamydial bodies and antigens in the central nervous system of patients with multiple sclerosis. J Infect Dis 192: 1219-1228.
51. Kong G-Y, Kristensson K, Bentivoglio M (2002) Reaction of mouse brain oligodendrocytes and their precursors, astrocytes and microglia, to proinflammatory medicators circulating in the cerebrospinal fluid. GLIA 37: 191-205.