Prospective isolation of bronchiolar stem cells based upon immunophenotypic and autofluorescence characteristics

Stem Cells

Volumn 27, Issue 3, 2009, Pages 612-622

  Teisanu, Roxana M ^a,b   Lagasse, Eric ^c   Whitesides, John F ^a   Stripp, Barry R ^a,b  

^a DUKE UNIVERSITY MEDICAL CENTER   (United States)

^b UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

^c UNIVERSITY OF PITTSBURGH   (United States)

Author keywords

Bronchioalveolar stem cell; Bronchiolar stem cell; Clara; Epithelium; Fractionation; Progenitor

Indexed keywords

CD31 ANTIGEN; CD34 ANTIGEN; CD45 ANTIGEN;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; AUTOFLUORESCENCE; BRONCHIOLE; CELL FRACTIONATION; CELL ISOLATION; CLARA CELL; CONTROLLED STUDY; IMMUNOPHENOTYPING; IMMUNOREACTIVITY; LUNG ALVEOLUS EPITHELIUM; MOUSE; NONHUMAN; STEM CELL;

ANIMALS; ANTIGENS, CD31; ANTIGENS, CD34; ANTIGENS, CD45; ANTIGENS, LY; BRONCHIOLES; CELLS, CULTURED; FLOW CYTOMETRY; FLUORESCENCE; FLUORESCENT ANTIBODY TECHNIQUE; IMMUNOPHENOTYPING; MEMBRANE PROTEINS; MICE; POLYMERASE CHAIN REACTION; STEM CELLS;

ANIMALIA;

EID: 65249098332     PISSN: 10665099     EISSN: None     Source Type: Journal    
DOI: 10.1634/stemcells.2008-0838     Document Type: Article

References (24)

1. Evans MJ, Johnson LV, Stephens RJ et al. Renewal of the terminal bronchiolar epithelium in the rat following exposure to NO2 or O3. Lab Invest 1976;35:246-257.
2. Evans MJ, Cabral-Anderson LJ, Freeman G. Role of the Clara cell in renewal of the bronchiolar epithelium. Lab Invest 1978;38:648-653.
3. Randell SH. Airway epithelial stem cells and the pathophysiology of chronic obstructive pulmonary disease. Proc Am Thorac Soc 2006;3: 718-725.
4. Stripp BR. Hierarchical organization of lung progenitor cells: Is there an adult lung tissue stem cell? Proc Am Thorac Soc 2008;5:695-698.
5. Rawlins EL, Hogan BL. Epithelial stem cells of the lung: Privileged few or opportunities for many? Development 2006;133:2455-2465.
6. Rawlins EL, Ostrowski LE, Randell SH et al. Lung development and repair: Contribution of the ciliated lineage. Proc Natl Acad Sci USA 2007;104:410-417.
7. Hong KU, Reynolds SD, Giangreco A et al. Clara cell secretory protein-expressing cells of the airway neuroepithelial body microenvironment include a label-retaining subset and are critical for epithelial renewal after progenitor cell depletion. Am J Respir Cell Mol Biol 2001;24:671-681.
8. Giangreco A, Reynolds SD, Stripp BR. Terminal bronchioles harbor a unique airway stem cell population that localizes to the bronchioalveolar duct junction. Am J Pathol 2002;161:173-182.
9. Stripp BR, Maxson K, Mera R et al. Plasticity of airway cell proliferation and gene expression after acute naphthalene injury. Am J Physiol 1995;269:L791-L799.
10. Kim CF, Jackson EL, Woolfenden AE et al. Identification of bronchioalveolar stem cells in normal lung and lung cancer. Cell 2005; 121:823-835.
11. Reynolds SD, Hong KU, Giangreco A et al. Conditional clara cell ablation reveals a self-renewing progenitor function of pulmonary neuroendocrine cells. Am J Physiol Lung Cell Mol Physiol 2000;278: L1256-L1263.
12. Reynolds SD, Zemke AC, Giangreco A et al. Conditional stabilization of beta-catenin expands the pool of lung stem cells. Stem Cells 2008; 26:1337-1346.
13. Srinivas S, Watanabe T, Lin CS et al. Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus. BMC Dev Biol 2001;1:4.
14. Soriano P. Generalized lacZ expression with the ROSA26 Cre reporter strain. Nat Genet 1999;21:70-71.
15. Chichester CH, Philpot RM, Weir AJ et al. Characterization of the cytochrome P-450 monooxygenase system in nonciliated bronchiolar epithelial (Clara) cells isolated from mouse lung. Am J Respir Cell Mol Biol 1991;4:179-186.
16. Kotton DN, Summer RS, Sun X et al. Stem cell antigen-1 expression in the pulmonary vascular endothelium. Am J Physiol Lung Cell Mol Physiol 2003;284:L990-L996.
17. Barker N, van Es JH, Kuipers J et al. Identification of stem cells in small intestine and colon by marker gene Lgr5. Nature 2007;449: 1003-1007.
18. Ikeda N, Hayashi A, Iwasaki K et al. Comprehensive diagnostic bronchoscopy of central type early stage lung cancer. Lung Cancer 2007;56:295-302.
19. Leonhard M. New incoherent autofluorescence/fluorescence system for early detection of lung cancer. Diagn Ther Endosc 1999;5:71-75.
20. Lam B, Wong MP, Fung SL et al. The clinical value of autofluorescence bronchoscopy for the diagnosis of lung cancer. Eur Respir J 2006;28:915-919.
21. Ventura JJ, Tenbaum S, Perdiguero E et al. p38alpha MAP kinase is essential in lung stem and progenitor cell proliferation and differentiation. Nat Genet 2007;39:750-758.
22. Zhang Y, Goss AM, Cohen ED et al. A Gata6-Wnt pathway required for epithelial stem cell development and airway regeneration. Nat Genet 2008;40:862-870.
23. Yang Y, Iwanaga K, Raso MG et al. Phosphatidylinositol 3-kinase mediates bronchioalveolar stem cell expansion in mouse models of oncogenic K-ras-induced lung cancer. PLoS ONE 2008;3:e2220.
24. You Y, Richer EJ, Huang T et al. Growth and differentiation of mouse tracheal epithelial cells: Selection of a proliferative population. Am J Physiol Lung Cell Mol Physiol 2002;283:L1315-L1321.