A naturally occurring splicing site mutation in the brassica rapa FLC1 gene is associated with variation in flowering time

Journal of Experimental Botany

Volumn 60, Issue 4, 2009, Pages 1299-1308

  Yuan, Yu Xiang ^a,b   Wu, Jian ^a   Sun, Ri Fei ^a   Zhang, Xiao Wei ^b   Xu, Dong Hui ^a   Bonnema, Guusje ^c   Wang, Xiao Wu ^a  

^a INSTITUTE OF PLANT PROTECTION   (China)

^b INSTITUTE OF PLANT PROTECTION   (China)

^c WAGENINGEN UNIVERSITY   (Netherlands)

Author keywords

BrFLC1; Flowering time; Splicing pattern; Splicing site mutation

Indexed keywords

VEGETABLE PROTEIN;

BIOLOGICAL MODEL; COLD; CONFERENCE PAPER; FLOWER; GENE EXPRESSION REGULATION; GENETIC MARKER; GENETICS; GENOTYPE; MUTATION; PHENOTYPE; PLANT GENE; REPRODUCIBILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA SPLICING; SINGLE NUCLEOTIDE POLYMORPHISM; TIME; TURNIP;

BRASSICA RAPA; COLD TEMPERATURE; FLOWERS; GENE EXPRESSION REGULATION, PLANT; GENES, PLANT; GENETIC MARKERS; GENOTYPE; MODELS, BIOLOGICAL; MUTATION; PHENOTYPE; PLANT PROTEINS; POLYMORPHISM, SINGLE NUCLEOTIDE; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA SPLICE SITES; RNA SPLICING; TIME FACTORS;

ARABIDOPSIS; BRASSICA; BRASSICA RAPA; BRASSICA RAPA SUBSP. PEKINENSIS; BRASSICA RAPA SUBSP. RAPA;

EID: 64949143286     PISSN: 00220957     EISSN: 14602431     Source Type: Journal    
DOI: 10.1093/jxb/erp010     Document Type: Conference Paper

References (46)

1. Ajisaka H, Kuginuki Y, Yui S, Enomoto S, Hirai M. 2001. Identification and mapping of a quantitative trait locus controlling extreme late bolting in Chinese cabbage (Brassica rapa L. ssp. pekinensis syn. campestris L.) using bulked segregant analysis. Euphytica 118, 75-81.
2. Alexandre CM, Hennig L. 2008. FLC or not FLC: the other side of vernalization. Journal of Experimental Botany 59, 1127-1135.
3. Axeisson T, Shavorskaya O, Lagercrantz U. 2001. Multiple flowering time QTLs within several Brassica species could be the result of duplicated copies of one ancestral gene. Genome 44, 856-864.
4. Boss PK, Bastow RM, Mylne JS, Dean C. 2004. Multiple pathways in the decision to flower: enabling, promoting and resetting. The Plant Cell 16, S18-S31.
5. Brown JWS. 1996. Arabidopsis intron mutations and pre-mRNA splicing. The Plant Journal 10, 771-780.
6. Caicedo AL, Stinchcombe JR, Olsen KM, Schmitt J, Purugganan MD. 2004. Epistatic interaction between Arabidopsis FRI and FLC flowering time genes generates a latitudinal cline in a life history trait. Proceedings of the National Academy of Sciences, USA 101, 15670-15675.
7. De Lucia F, Crevillen P, Jones AM, Greb T, Dean C. 2008. A PHD-polycomb repressive complex 2 triggers the epigenetic silencing of FLC during vernalization. Proceedings of the National Academy of Sciences, USA 105, 16831-16836.
8. Gómez-Campo C. 1999. Biology of Brassica coenospecies, Vol. 4. Amsterdam: Elsevier, 33-52.
9. He Y, Amasino RM. 2005. Role of chromatin modification in flowering-time control. Trends in Plant Science 10, 30-35.
10. Hu X, Sullivan-Gilbert M, Gupta M, Thompson SA. 2007. G-to-A mutation at a 5′ splice site of fad3c caused impaired splicing in a low linolenic mutant of canola (Brassica napus L.). Plant Biotechnology 24, 397-400.
11. Jack T. 2004. Molecular and genetic mechanisms of floral control. The Plant Cell 16, S1-S17.
12. Kim J, Chung T, King G, Jin M, Yang T, Jin Y, Kim H, Park B. 2006. A sequence-tagged linkage map of Brassica rapa. Genetics 174, 29-39.
13. Kim SY, Park BS, Kwon SJ, et al. 2007. Delayed flowering time in Arabidopsis and Brassica rapa by the overexpression of FLOWERING LOCUS C (FLC) homologs isolated from Chinese cabbage (Brassica rapa L. ssp. pekinensis). Plant Cell Reports 26, 327-336.
14. Kole C, Quijada P, Michaels SD, Amasino RM, Osborn TC. 2001. Evidence for homology of flowering-time genes VFR2 from Brassica rapa and FLC from Arabidopsis thaliana. Theoretical and Applied Genetics 102, 425-430.
15. Lagercrantz U, Osterberg MK, Lascoux M. 2002. Sequence variation and haplotype structure at the putative flowering-time locus COL1 of Brassica nigra. Molecular Biology and Evolution 19, 1474-1482.
16. Lee I, Amasino RM. 1995. Effect of vernalization, photoperiod and light quality on the flowering phenotype of Arabidopsis plants containing the FRIGIDA gene. Plant Physiology 108, 157-162.
17. Lempe J, Balasubamanian S, Sureshkumar S, Singh A, Schmid M, Weigel D. 2005. Diversity of flowering responses in wild Arabidopsis thaliana strain. PLoS Genetics 1, 109-118.
18. Li G, Quiros CF. 2002. Genetic analysis, expression and molecular characterization of BoGSL-ELONG, a major gene involved in the aliphatic glucosinolate pathway of Brassica species. Genetics 162, 1937-1943.
19. Li Z, Zhao L, Cui C, Kai G, Zhang L, Sun X, Tang K. 2005. Molecular cloning and characterization of anti-bolting related gene (BrpFLC) from Brassica rapa ssp. Pekinensis. Plant Science 168, 407-413.
20. Lin SI, Wang JG, Poon SY, Su CI, Wang SS, Chiou TJ. 2005. Differential regulation of FLOWERING LOCUS C expression by vernalization in cabbage and Arabidopsis. Plant Physiology 137, 1037-1048.
21. Lorkovic ZJ, Kirk DAW, Lambermon MHL, Filipowicz W. 2000. Pre-mRNA splicing in higher plants. Trends in Plant Science 5, 160-167.
22. Lou P, Zhao J, Kim JS, et al. 2007. Quantitative trait loci for flowering time and morphological traits in multiple populations of Brassica rapa. Journal of Experimental Botany 58, 4005-4016.
23. McCullough AJ, Lou H, Schuler MA. 1993. Factors affecting authentic 5′ splice site selection in higher plants. Trends in Plant Science 5, 160-167.
24. Michaels SD, Amasino RM. 1999. FLOWERING LOCUS C encodes a novel MADS domain protein that acts as a repressor of flowering. The Plant Cell 11, 949-956.
25. Mouradov A, Cremer F, Coupland G. 2002. Control of flowering time: interacting pathways as a basis for diversity. The Plant Cell 14, S111-S130.
26. Okazaki K, Sakamoto K, Kikuchi R, Saito A, Togashi E, Kuginuki Y, Matsumoto S, Hirai M. 2007. Mapping and characterization of FLC homologs and QTL analysis of flowering time in Brassica oleracea. Theoretical and Applied Genetics 114, 595-608.
27. Osborn TC, Kole C, Parkin IAP, Sharpe AG, Kuiper M, Lydiate DJ, Trick M. 1997. Comparison of flowering time genes in Brassica rapa, B. Napus and Arabidopsis thaliana. Genetics 146, 1123-1129.
28. Osterberg M, Shavorskaya O, Lascoux M, Lagercrantz U. 2002. Naturally occurring indel variation in the Brassica nigra COL1 gene is associated with variation in flowering time. Genetics 161, 299-306.
29. Razi H, Howell EC, Newbury HJ, Kearsey MJ. 2008. Does sequence polymorphism of FLC paralogues underlie flowering time QTL in Brassica oleracea? Theoretical and Applied Genetics 116, 179-192.
30. Rouse DT, Sheldon CC, Bagnall DJ, Peacock WJ, Dennis ES. 2002. FLC, a repressor of flowering, is regulated by genes in different inductive pathways. The Plant Journal 29, 183-191.
31. Schmitz RJ, Amasino RM. 2007. Vernalization: a model for investigating epigenetics and eukaryotic gene regulation in plants. Biochimica et Biophysica Acta 1769, 269-75.
32. Schranz ME, Quijada P, Sung SB, Lukens L, Amasino R, Osborn TC. 2002. Characterization and effects of the replicated flowering time gene FLC in Brassica rapa. Genetics 162, 1457-1468.
33. Sheldon CC, Burn JE, Perez PP, Metzger J, Edwards JA, Peacock WJ, Dennis ES. 1999. The FLF MADS box gene: a repressor of flowering in Arabidopsis regulated by vernalization and methylation. The Plant Cell 11, 445-458.
34. Sheldon CC, Hills MJ, Lister C, Dean C, Dennis ES, Peacock WJ. 2008. Resetting of FLOWERING LOCUS C expression after epigenetic repression by vernalization. Proceedings of the National Academy of Sciences, USA 105, 2214-2219.
35. Sheldon CC, Rouse DT, Finnegan EJ, Peacock WJ, Dennis ES. 2000. The molecular basis of vernalization: the central role of FLOWERING LOCUS C (FLC). Proceedings of the National Academy of Sciences, USA 97, 3753-3758.
36. Simpson GG, Gendall AR, Dean C. 1999. When to switch to flowering. Annual Review of Cell and Developmental Biology 99, 519-550.
37. Simpson CG, McQuade C, Lyon J, Brown JWS. 1998. Characterization of exon skipping mutants of the COP1 gene from Arabidopsis. The Plant Journal 15, 125-131.
38. Sung S, Amasino RM. 2005. Remembering winter: toward a molecular understanding of vernalization. Annual Review of Plant Biology 56, 491-508.
39. Sung S, Amasino RM. 2006. Molecular genetic studies of the memory of winter. Journal of Experimental Botany 57, 3369-3377.
40. Tadege M, Sheldon CC, Helliwell CA, Stoutjesdijk P, Dennis ES, Peacock WJ. 2001. Control of flowering time by FLC orthologues in Brassica napus. The Plant Journal 28, 545-553.
41. Teutonico R, Osborn T. 1994. Mapping of RFLP and quantitative trait loci in Brassica rapa and comparison to the linkage maps of B. napus, B. oleracea, and Arabidopsis thaliana. Theoretical and Applied Genetics 89, 885-894.
42. Wang XW, Lou P, He HJ, Yang BJ, Zhang YG. 2005. Construction of an AFLP-based genetic linkage map using a double-haploid (DH) population from a cross between Chinese kale and Broccoli. Acta Horticulture Sinica 32, 30-34.
43. Werner JD, Borevitz JO, Uhlenhaut NH, Ecker JR, Chory J, Weigel D. 2005. FRIGIDA-Independent variation in flowering time of natural Arabidopsis thaliana accessions. Genetics 170, 1197-1207.
44. Wu J, Yuan Y, Zhao J, Song X, Li Y, Li X, Sun R, Koornneef M, Aarts MGM, Wang X. 2008. Mapping QTLs for mineral accumulation and shoot dry biomass yield under different Zn nutritional conditions in Chinese cabbage (Brassica rapa L. ssp. pekinensis). Plant and Soil 310, 25-40.
45. Yang TJ, Kim JS, Kwon SJ, et al. 2006. Sequence-level analysis of the diploidization process in the triplicated FLOWERING LOCUS C region of Brassica rapa. The Plant Cell 18, 1339-1347.
46. Zhao J, Paulo M-J, Jamar D, Lou P, Fv Eeuwijk, Bonnema G, Vreugdenhil D, Koornneef M. 2007. Association mapping of leaf traits, flowering time, and phytate content in Brassica rapa. Genome 50, 963-973.