Hierarchical oligonucleotide primer extension as a time- and cost-effective approach for quantitative determination of Bifidobacterium spp. in Infant feces

Applied and Environmental Microbiology

Volumn 75, Issue 8, 2009, Pages 2573-2576

  Hong, Pei Ying ^a   Yap, Gaik Chin ^b   Lee, Bee Wah ^c   Chua, Kaw Yan ^c   Liu, Wen Tso ^a,c  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^b NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^c University of Illinois at Urbana Champaign   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

16S RRNA GENES; BIFIDOBACTERIA; BIFIDOBACTERIUM; BIFIDOBACTERIUM LONGUM; COST-EFFECTIVE APPROACHES; FECAL SAMPLES; FLUORESCENCE IN SITU HYBRIDIZATIONS; HIGH THROUGHPUTS; OLIGONUCLEOTIDE PRIMERS; P VALUES; QUANTITATIVE DETERMINATIONS; RELATIVE ABUNDANCES; STATISTICAL CORRELATIONS;

COST EFFECTIVENESS; FLOW CYTOMETRY; FLUORESCENCE MICROSCOPY;

OLIGONUCLEOTIDES;

OLIGONUCLEOTIDE; RIBOSOME RNA;

ABUNDANCE; BACTERIUM; FECES; FLOW CYTOMETRY;

ARTICLE; BACTERIUM IDENTIFICATION; BIFIDOBACTERIUM; BIFIDOBACTERIUM ADOLESCENTIS; BIFIDOBACTERIUM BIFIDUM; BIFIDOBACTERIUM BREVE; BIFIDOBACTERIUM CATENULATUM; BIFIDOBACTERIUM LONGUM; COMPARATIVE STUDY; CORRELATION ANALYSIS; COST EFFECTIVENESS ANALYSIS; FECES ANALYSIS; FLOW CYTOMETRY; FLUORESCENCE IN SITU HYBRIDIZATION; GENE AMPLIFICATION; GENE SEQUENCE; HIGH THROUGHPUT SCREENING; HUMAN; HYBRIDIZATION; NONHUMAN; QUANTITATIVE ANALYSIS;

BIFIDOBACTERIUM; COLONY COUNT, MICROBIAL; DNA PRIMERS; FECES; FLOW CYTOMETRY; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; INFANT; RNA, BACTERIAL; RNA, RIBOSOMAL, 16S; TIME FACTORS;

BACTERIA (MICROORGANISMS); BIFIDOBACTERIUM; BIFIDOBACTERIUM ADOLESCENTIS; BIFIDOBACTERIUM BIFIDUM; BIFIDOBACTERIUM BREVE; BIFIDOBACTERIUM CATENULATUM; BIFIDOBACTERIUM LONGUM;

EID: 64749104761     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.02576-08     Document Type: Article

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