Quantitative analysis of intracellular sugar phosphates and sugar nucleotides in encapsulated streptococci using HPAEC-PAD

Biotechnology Journal

Volumn 4, Issue 1, 2009, Pages 58-63

  Marcellin, Esteban ^a   Nielsen, Lars K ^a   Abeydeera, Peter ^a   Krömer, Jens O ^a  

^a UNIVERSITY OF QUEENSLAND   (Australia)

Author keywords

HPAEC PAD; Hyaluronic acid; Metabolomics; Nucleotide sugars; Streptococcus zooepidemicus

Indexed keywords

HPAEC-PAD; HYALURONIC ACID; METABOLOMICS; NUCLEOTIDE SUGARS; STREPTOCOCCUS ZOOEPIDEMICUS;

BIOLOGICAL SYSTEMS; BIOMASS; CELL CULTURE; CENTRIFUGATION; CENTRIFUGES; CHROMATOGRAPHIC ANALYSIS; COLUMN CHROMATOGRAPHY; HARVESTING; HYDROXYLATION; METABOLISM; METABOLITES; NUCLEIC ACIDS; NUCLEOTIDES; ORGANIC ACIDS; PHOSPHATES;

SUGAR (SUCROSE);

HYALURONIC ACID; SUGAR NUCLEOTIDE; SUGAR PHOSPHATE;

AMPEROMETRY; ANALYTIC METHOD; ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; BIOMASS; CELL ISOLATION; CELL SEPARATION; CENTRIFUGATION; CONTROLLED STUDY; DRY WEIGHT; ENCAPSULATION; EXTRACTION; HYPOTHESIS; LIQUID CULTURE; METABOLOME; METABOLOMICS; MICROORGANISM; NONHUMAN; NUTRIENT; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; STREPTOCOCCUS; STREPTOCOCCUS EQUI ZOOEPIDEMICUS;

AMINES; BACTERIAL CAPSULES; CENTRIFUGATION; CHROMATOGRAPHY, ION EXCHANGE; METABOLOMICS; SOLID PHASE EXTRACTION; STREPTOCOCCUS EQUI; SUGAR PHOSPHATES; URIDINE DIPHOSPHATE SUGARS;

BACTERIA (MICROORGANISMS); STREPTOCOCCUS EQUI SUBSP. ZOOEPIDEMICUS;

EID: 64549142471     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.200800197     Document Type: Article

References (18)

1. Letisse, F., Lindley, N. D., An intracellular metabolite quantification technique applicable to polysaccharide-producing bacteria. Biotechnol. Lett. 2000, 22, 1673-1677.
2. Blank, L. M., Fong Chong, B., McLaughlin, R., Nielsen, L. K., Microbial hyaluronic acid production. Appl. Microbiol. Biotechnol. 2005, 66, 341-351.
3. Bordes-Benítez, A., Sánchez-Oñoro, M., Suárez-Bordón, P., García-Rojas, A., et al., Outbreak of Streptococcus equi subsp. zooepidemicus infections on the island of Gran Canaria associated with the consumption of inadequately pasteurized cheese. Eur. J. Clin. Microbiol. Infect. Dis. 2006, 25, 242-246.
4. O'Brien, K. L., Beall, B., Barrett, N. L., Cieslak, P. R. et al., Epidemiology of invasive group A Streptococcus disease in the United States, 1995-1999. Clin. Infect. Dis. 2002, 35, 268-276.
5. Armstrong, D. C., Cooney, M. J., Johns, M. R., Growth and amino acid requirements of hyaluronic-acid-producing Streptococcus zooepidemicus. Appl. Microbiol. Biotechnol. 1997, 47, 309-312.
6. van de Rijn, I., Kessler, R. E., Growth characteristics of group A streptococci in a new chemically defined medium. Infect. Immun. 1980, 27, 444-448.
7. Prasad Maharjan, R., Ferenci, T., Global metabolite analysis: The influence of extraction methodology on metabolome profiles of Escherichia coli. Anal. Biochem. 2003, 313, 145-154.
8. De Koning, W., Van Dam, K., A method for the determination of changes of glycolytic metabolites in yeast on a subsecond time scale using extraction at neutral pH. Anal. Biochem. 1992, 204, 118-123.
9. Gonzalez, B., Renaud, J. F. M., A rapid and reliable method for metabolite extraction in yeast using boiling buffered ethanol. Yeast 1997, 13, 1347-1355.
10. Hajjaj, H., Blanc, P. J., Goma, G., François, J., Sampling techniques and comparative extraction procedures for quantitative determination of intra- and extracellular metabolites in filamentous fungi. FEMS Microbiol. Lett. 1998, 164, 195-200.
11. Wittmann, C., Krömer, J. O., Kiefer, P., Binz, T., Heinzle, E., Impact of the cold shock phenomenon on quantification of intracellular metabolites in bacteria. Anal. Biochem. 2004, 327, 135-139.
12. Link, H., Anselment, B., Weuster-Botz, D., Leakage of adenylates during cold methanol/glycerol quenching of Escherichia coli. Metabolomics (in press) doi: 10.1007/s11306-008-0114-6 2008.
13. Marcellin, E., Chen, W., Nielsen, L. K., Microbial hyaluronic acid biosynthesis, in: Rehm, B. H. A. (Ed.), Microbial Production of Biopolymers and Polymer Precursors, Horizon press, Norwich (in press).
14. Fong Chong, B., Nielsen, L. K., Amplifying the cellular reduction potential of Streptococcus zooepidemicus. J. Biotechnol. 2003, 100, 33-41.
15. Villas-Boas, S. G., Bruheim, P., Cold glycerol-saline: The promising quenching solution for accurate intracellular metabolite analysis of microbial cells. Anal. Biochem. 2007, 370, 87-97.
16. Mclaughlin, R., Hyaluronic acid production in continuous cultures of Streptococcus zooepidemicus, PhD thesis, Department of Chemical Engineering, The University of Queensland, Brisbane 2005.
17. Jensen, N. B. S., Jokumsen, K. V., Villadsen, J., Determination of the phosphorylated sugars of the Embden-Meyerhoff-Parnas pathway in Lactococcus lactis using a fast sampling technique and solid phase extraction. Biotechnol. Bioeng. 1999, 63, 356-362.
18. Bolten, C. J., Kiefer, P., Letisse, F., Portais, J. C., Wittmann, C., Sampling for metabolome analysis of microorganisms. Anal. Chem. 2007, 79, 3843-3849.