Evaluation of fluorescence-based methods for total vs. amplifiable DNA quantification in plasma of lung cancer patients

Journal of Physiology and Pharmacology

Volumn 59, Issue SUPPL. 6, 2008, Pages 675-681

  Szpechcinski, A ^a,c   Struniawski, R ^a   Zaleska, J ^b   Chabowski, M ^c   Orlowski, T ^c   Roszkowski, K ^b   Chorostowska Wynimko, J ^a  

^a Laboratory of Molecular Diagnostics   (Poland)

^b Department of Lung Diseases ^*  (Poland)

^c NATIONAL TUBERCULOSIS AND LUNG DISEASES RESEARCH INSTITUTE   (Poland)

Author keywords

DNA quantification; Lung cancer; PicoGreen; Plasma DNA; Real time PCR

Indexed keywords

DNA; FLUOROCHROME; FLUORESCENT DYE; ORGANIC COMPOUND; PICOGREEN;

ARTICLE; CANCER PATIENT; CLINICAL ARTICLE; FLUORESCENCE ANALYSIS; GENE AMPLIFICATION; HUMAN; LUNG NON SMALL CELL CANCER; REAL TIME POLYMERASE CHAIN REACTION; BLOOD; LUNG TUMOR; METHODOLOGY; REPRODUCIBILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

CARCINOMA, NON-SMALL-CELL LUNG; DNA, NEOPLASM; FLUORESCENT DYES; HUMANS; LUNG NEOPLASMS; ORGANIC CHEMICALS; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;

EID: 64549135075     PISSN: 08675910     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (12)

1. Swarup V, Rajeswari MR. Circulating (cell-free) nucleic acids-a promising, non-invasive tool for early detection of several human diseases. FEBS Lett 2007; 581: 795-799.
2. Herrera LJ, Raja S, Gooding WE et al. Quantitative analysis of circulating plasma DNA as a tumor marker in thoracic malignancies. Clin Chem 2005; 51: 113-118.
3. Chorostowska-Wynimko J, Szpechcinski A. The impact of genetic markers on the diagnosis of lung cancer: a current perspective. J Thorac Oncol 2007; 2: 1044-1051.
4. Holdenrieder S, Stieber P, Bodenmüller H et al. Nucleosomes in serum of patients with benign and malignant diseases. Int J Cancer 2001; 95: 114-120.
5. Jiang N, Pisetsky DS. The effect of inflammation on the generation of plasma DNA from dead and dying cells in the peritoneum. J Leukoc Biol 2005; 77: 296-302.
6. Chen JA, Meister S, Urbonaviciute V et al. Sensitive detection of plasma/serum DNA in patients with systemic lupus erythrematous. Autoimmunity 2007; 40: 307-310.
7. Xie GS, Hou AR, Li LY, Gao YN, Cheng SJ. Quantification of plasma DNA as a screening tool for lung cancer. Chin Med J 2004; 117: 1485-1488.
8. Ahn SJ, Costa J, Emanuel JR. PicoGreen quantitation of DNA: effective evaluation of samples pre- or post-PCR. Nucleic Acids Res 1996; 24: 2623-2625.
9. Sozzi G, Conte D, Mariani L et al. Analysis of circulating tumor DNA in plasma at diagnosis and during follow-up of lung cancer patients. Cancer Res 2001; 61: 4675-4678.
10. Sozzi G, Conte D, Leon M et al. Quantification of free circulating DNA as a diagnostic marker in lung cancer. J Clin Oncol 2003; 21: 3902-3908.
11. Chiminqgi M, Moutereau S, Pernet P et al. Specific real-time PCR vs. fluorescent dyes for serum free DNA quantification. Clin Chem Lab Med 2007; 45: 993-995.
12. Blotta I, Prestinaci F, Mirante S, Cantafora A. Quantitative assay of total dsDNA with PicoGreen reagent and real-time fluorescent detection. Ann Ist Super Sanita 2005; 41: 119-123.