The hydrophobic tunnel present in LOX-1 is essential for oxidized LDL recognition and binding

Journal of Lipid Research

Volumn 50, Issue 3, 2009, Pages 546-555

  Francone, Omar L ^a   Tu, Meihua ^a   Royer, Lori J ^a   Zhu, Jian ^a   Stevens, Kimberly ^a   Oleynek, Joseph J ^a   Lin, Zhiwu ^a   Shelley, Lorraine ^a   Sand, Thomas ^a   Luo, Yi ^a   Kane, Christopher D ^a  

^a PFIZER INC   (United States)

Author keywords

Endothelial cells; Lipoprotein oxidation; Receptors; Scavenger

Indexed keywords

GLUTAMIC ACID; ISOLEUCINE; LOW DENSITY LIPOPROTEIN; OXIDIZED LOW DENSITY LIPOPROTEIN RECEPTOR 1; PHENYLALANINE; TYROSINE; OLR1 PROTEIN, HUMAN; OXIDIZED LOW DENSITY LIPOPROTEIN; RECOMBINANT PROTEIN;

AMINO ACID SUBSTITUTION; ANIMAL CELL; ARTICLE; BINDING SITE; CONTROLLED STUDY; FEMALE; HYDROPHOBICITY; MUTANT; NONHUMAN; NUCLEOTIDE SEQUENCE; OXIDATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN STRUCTURE; WILD TYPE; ANIMAL; CHEMICAL STRUCTURE; CHEMISTRY; CHO CELL; CRICETULUS; GENETIC TRANSFECTION; GENETICS; HAMSTER; HUMAN; METABOLISM; MOLECULAR CLONING; SITE DIRECTED MUTAGENESIS;

AMINO ACID SUBSTITUTION; ANIMALS; BINDING SITES; CHO CELLS; CLONING, MOLECULAR; CRICETINAE; CRICETULUS; HUMANS; HYDROPHOBICITY; LIPOPROTEINS, LDL; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; RECOMBINANT PROTEINS; SCAVENGER RECEPTORS, CLASS E; TRANSFECTION;

EID: 64349114841     PISSN: 00222275     EISSN: 15397262     Source Type: Journal    
DOI: 10.1194/jlr.M800474-JLR200     Document Type: Article

References (23)

1. Sawamura, T., N. Kume, T. Aoyama, H. Moriwaki, H. Hosikawa, Y. Aiba, T. Tanaka, S. Miwa, Y. Katsura, T. Kita, et al. 1997. An endothelial receptor for oxidized low-density lipoprotein-receptor in human atherosclerosis lesions. Nature. 386: 73-77.
2. Yoshida, H., N. Kondratenko, S. Green, D. Steinberg, and O. Quehenberger. 1998. Identification of the lectin-like receptor for oxidized low-density lipoprotein in human macrophages and its potential role as a scavenger receptor. Biochem. J. 334: 9-13.
3. Draude, G., N. Hrboticky, and R. L. Lorenz. 1999. The expression of the lectin-like oxidized low-density lipoprotein receptor (LOX-1) on human vascular smooth muscle cells and monocytes and its down-regulation by lovastatin. Biochem. Pharmacol. 57: 383-386.
4. Chen, M., M. Kakutani, T. Naruko, M. Ueda, S. Narumiya, T. Masaki, and T. Sawamura. 2001. Activation-dependent surface expression of LOX-1 in human platelets. Biochem. Biophys. Res. Commun. 282: 153-158.
5. Kume, N., T. Murase, H. Moriwaki, T. Aoyama, T. Sawamara, T. Masaki, and T. Kita. 1998. Inducible expression of lectin-like oxidized LDL receptor-1 in vascular endothelial cells. Circ. Res. 83: 322-327.
6. Murase, T., N. Kume, R. Korenaga, J. Ando, T. Sawamara, T. Masaki, and T. Kita. 1998. Fluid shear stress transcriptionally induces lectin-like oxidized LDL receptor-1 in vascular endothelial cells. Circ. Res. 83: 328-333.
7. Inoue, K., Y. Arai, H. Kurihara, T. Kita, and T. Sawamara. 2005. Overexpression of lectin-like oxidized low-density lipoprotein receptor-1 induces intramyocardial vasculopathy in apolipoprotein E-null mice. Circ. Res. 97: 176-184.
8. Mehta, J. L., N. Sanada, C. P. Hu, J. Chen, A. Dandapat, F. Sugawara, H. Satoh, K. Inoue, Y. Kawase, K. Jishage, et al. 2007. Deletion of LOX-1 reduces atherogenesis in LDLR knockout mice fed high cholesterol diet. Circ. Res. 100: 1634-1642.
9. Tatsuguchi, M., M. Furutani, J. Hinagata, T. Tanaka, Y. Furutani, S. Imamura, M. Kawana, T. Masaki, H. Kasanuki, T. Sawamura, et al. 2003. Oxidized LDL receptor gene (ORL1) is associated with the risk of myocardial infarction. Biochem. Biophys. Res. Commun. 303: 247-250.
10. Mango, R., S. Biocca, F. del Vecchio, F. Clementi, F. Sangiuolo, F. Amati, A. Filareto, S. Grelli, P. Spitalieri, I. Filesi, et al. 2005. In vivo and in vitro studies support that a new splicing isoform of OLR1 gene is protective against acute myocardial infarction. Circ. Res. 97: 152-158.
11. Puccetti, L., A. L. Pasqui, F. Bruni, M. Pastorelli, F. Ciani, A. Palazzuoli, A. Pontani, A. Ghezzi, and A. Auteri. 2006. Lectin-like oxidized LDL receptor-1 (LOX-1) polymorphisms influence cardiovascular events rate during statin treatment. Int. J. Cardiol. 119: 41-47.
12. Chen, M., K. Inoue, S. Narumiya, T. Masaki, and T. Sawamura. 2001. Requirements of basic amino acids residues within the lectin-like domain of LOX-1 for the binding of oxidized low-density lipoprotein. FEBS Lett. 499: 215-219.
13. Chen, M., S. Narumiya, T. Masaki, and T. Sawamura. 2001. Conserved C-terminal residues within the lectin-like domain of LOX-1 are essential for oxidized low-density-lipoprotein binding. Biochem. J. 355: 289-296.
14. Shi, X., S. Ogawa, T. Otani, and S. Machida. 2001. Involvement of conserved hydrophobic residues in the CTLD of human lectin-like oxidized LDL receptor in ligand binding. Mol. Cell Biol. Res. Commun. 4: 292-298.
15. Xie, Q., S. Matsunaga, S. Niimi, S. Ogawa, K. Tokuyasu, Y. Sakakibara, and S. Machida. 2004. Human lectin-like oxidized low-density lipoprotein receptor-1 functions as a dimer in living cells. DNA Cell Biol. 23: 111-117.
16. Ishigaki, T., I. Ohki, N. Utsunomiya-Tate, and S. I. Tate. 2007. Chimeric structural stabilities in the coiled-coil structure of the NECK domain in human lectin-like oxidized low-density lipoprotein receptor 1 (LOX-1). J. Biochem. 141: 855-866.
17. Matsunaga, S., Q. Xie, M. Kumano, S. Niimi, K. Sekizawa, Y. Sakakibara, S. Komba, and S. Machida. 2007. Lectin-like oxidized low-density lipoprotein receptor (LOX-1) functions as an oligomer and oligomerization is dependent on receptor density. Exp. Cell Res. 313: 1203-1214.
18. Park, H., F. G. Adsit, and J. C. Boyington. 2005. The 1.4 angstrom crystal structure of the human oxidized low density lipoprotein receptor lox-1. J. Biol. Chem. 280: 13593-13599.
19. Ohki, I., T. Ishigaki, T. Oyama, S. Matsunaga, Q. Xie, M. Ohsishi-Kameyama, T. Murata, D. Tsuchiya, S. Machida, K. Morikawa, et al. 2005. Crystal structure of human lectin-like, oxidized lowdensity lipoprotein receptor 1 ligand binding domain and its ligand recognition mode to oxLDL. Structure. 13: 905-917.
20. Nagamatsu, S., J. M. Kornhauser, C. F. Burant, S. Seino, K. E. Mayo, and G. I. Bell. 1992. Glucose transporter expression in brain. cDNA sequence of mouse GLUT3, the brain facilitative glucose transporter isoform, and identification of sites of expression by in situ hybridization. J. Biol. Chem. 267: 467-472.
21. Karplus, P. A. 1997. Hydrophobicity regained. Protein Sci. 6: 1302-1307.
22. Watson, A. D., N. Leitinger, M. Navab, K. F. Faull, S. Hörkkö, J. L. Witztum, W. Palinski, D. Schwenke, R. G. Salomon, W. Sha, et al. 1997. Structural identification by mass spectrometry of oxidized phospholipids in minimally oxidized low density lipoprotein that induces monocyte/endothelial interactions and evidence for their presence in vivo. J. Biol. Chem. 272: 13597-13607.
23. Endemann, G., L. W. Stanton, K. S. Madden, C. M. Bryant, R. T. White, and A. A. Protter. 1993. CD36 is a receptor for oxidized low density lipoprotein. J. Biol. Chem. 268: 11811-11816.