Analysis of Different Staining Techniques for C4d Detection in Renal Allograft Biopsies

Transplantation Proceedings

Volumn 41, Issue 3, 2009, Pages 862-865

  Ludovico Martins, H ^a   Silva, C ^a   Teodoro, W R ^a   Martini Filho, D ^b   Noronha, I L ^a,b  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

^b HOSPITAL BENEFICÊNCIA PORTUGUESA DE SÃO PAULO   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENT COMPONENT C4D; PARAFFIN;

ACUTE GRAFT REJECTION; ADOLESCENT; ADULT; ARTICLE; CHRONIC ALLOGRAFT NEPHROPATHY; CLINICAL ARTICLE; CONTROLLED STUDY; FEMALE; FROZEN SECTION; GOLD STANDARD; HUMAN; IMMUNOHISTOCHEMISTRY; KIDNEY ALLOGRAFT; KIDNEY BIOPSY; KIDNEY GRAFT REJECTION; MALE; PRIORITY JOURNAL;

ADULT; ANTIBODIES, MONOCLONAL; BIOPSY; CADAVER; COLORING AGENTS; COMPLEMENT C4B; FEMALE; GRAFT REJECTION; HUMANS; ISOANTIBODIES; KIDNEY TRANSPLANTATION; LIVING DONORS; MALE; MIDDLE AGED; PEPTIDE FRAGMENTS; RETROSPECTIVE STUDIES; TISSUE DONORS; TRANSPLANTATION, HOMOLOGOUS; YOUNG ADULT;

EID: 64349091296     PISSN: 00411345     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.transproceed.2009.01.073     Document Type: Article

References (13)

1. Feucht H.E. Complement C4d in graft capillaries-the missing link in the recognition of humoral alloreactivity. Am J Transplant 3 (2003) 646
2. Collins A.B., Schneeberger E.E., Pascual M.A., et al. Complement activation in acute humoral renal allograft rejection: diagnostic significance of C4d deposits in peritubular capillaries. J Am Soc Nephrol 10 (1999) 2208
3. Crespo M., Pascual M., Tolkoff-Rubin N., et al. Acute humoral rejection in renal allograft recipients. I. Incidence, serology and clinical characteristics. Transplantation 71 (2001) 652
4. Mauiyyedi S., Crespo M., Collins A.B., et al. Acute humoral rejection in kidney transplantation. II. Morphology, immunopathology, and pathologic classification. J Am Soc Nephrol 13 (2002) 779
5. Solez K., Colvin R.B., Racusen L.C., et al. Banff 07 classification of renal allograft pathology: updates and future directions. Am J Transplant 8 (2008) 753
6. Nadasdy G.M., Bott C., Cowden D., et al. Comparative study for the detection of peritubular capillary C4d deposition in human renal allografts using different methodologies. Hum Pathol 36 (2005) 1178
7. Troxell M.L., Weintraub L.A., Higgins J.P., et al. Comparison of C4d immunostaining methods in renal allograft biopsies. J Am Soc Nephrol 1 (2006) 583
8. Suzuki T., Horita S., Kadoya K., et al. C4d immunohistochemistry in glomerulonephritis with different antibodies. Clin Exp Nephrol 11 (2007) 287
9. Seemayer C.A., Gaspert A., Nickeleit V., et al. C4d staining of allograft biopsies: a comparative analysis of different staining techniques. Nephrol Dial Transplant 22 (2007) 568
10. Feucht H.E., and Mihatsch M.J. Diagnostic value of C4d in renal biopsies. Curr Opin Nephrol Hypertens 14 (2005) 592
11. Regele H., Exner M., Watschinger B., et al. Endothelial C4d deposition is associated with inferior kidney allograft outcome independently of cellular rejection. Nephrol Dial Transplant 16 (2001) 2058
12. Regele H., Böhmig G.A., Habicht A., et al. Capillary deposition of complement split product C4d in renal allograft is associated with basement membrane injury in peritubular and glomerular capillaries: a contribution of humoral immunity to chronic allograft rejection. J Am Soc Nephrol 13 (2002) 2371
13. David-Neto E., Prado E., Beutel A., et al. C4d-positive chronic rejection: a frequent entity with a poor outcome. Transplant 84 (2007) 1391