Detection and differentiation of Entamoeba histolytica and Entamoeba dispar by polymerase chain reaction in a community in Zulia State, Venezuela;Detección y diferenciación de Entamoeba histolytica y Entamoeba dispar mediante reacción en cadena de la polimerasa en individuos de una comunidad del Estado Zulia, Venezuela

Cadernos de Saude Publica

Volumn 25, Issue 1, 2009, Pages 151-159

  Rivero, Zulbey ^a,c   Bracho, Angela ^a   Calchi, Marinella ^a   Díaz, Iris ^a   Acurero, Ellen ^a   Maldonado, Adriana ^a   Chourio, Glenis ^a   Arráiz, Nailet ^a   Corzo, Gilbert ^b  

^a Escuela de Bioanálisis   (Venezuela)

^b Fallecido ^*

^c UNIVERSIDAD DEL ZULIA   (Venezuela)

Author keywords

Entamoeba; Feces; Microscopy; Parasites; Polymerase chain reaction

Indexed keywords

EID: 64249097995     PISSN: 0102311X     EISSN: 16784464     Source Type: Journal    
DOI: 10.1590/s0102-311x2009000100016     Document Type: Article

References (37)

1. Martínez-Palomo A, González-Robles A, De La Torre M. Selective agglutination of pathogenic strains of Entamoeba histolytica induced by con A. Nat New Biol 1973; 245:186-7.
2. Sargeaunt PG, Willians JE, Grene JD. The differentiation of invasive and non-invasive Entamoeba histolytica by isoenzyme electrophoresis. Trans R Soc Trop Med Hyg 1978; 72:519-21.
3. Petri Jr. WA, Clark CG, Diamond LS. Host-parasite relationships in amebiasis: conference report. J Infect Dis 1994; 169:483-4.
4. Brumpt E. Etude sommaire de l'Entamoeba dispar n.s.p. amibe à kystes quadrine de 1'homme. Bulletin de l'Academie de Médecine 1925; 94:943-52.
5. World Health Organization/Pan American Health Organization/United Nations Educational, Scientific and Cultural Organization. Report of a consultation of experts on amoebiasis. Mexico DF: World Health Organization/Pan American Health Organization/United Nations Educational, Scientific and Cultural Organization; 1997.
6. Shibayama-Hernández H, Pedroza-Gómez J, Rivero-Baños B, Shibayama M, Serrano-Luna J, Tsutsumi V A simple stool concentration method for the detection and preservation of the vegetative forms of Entamoeba histolytical Entamoeba dispar. Arch Med Res 2000; 31:S30-1.
7. th Ed. Washington DC: ASM Press; 2003.
8. Ritchie L. An ether sedimentation technique for routine stool examination. Bull U S Army Med Dep 1948; 8:326.
9. Núñez Y, Fernández M, Torres D, Silva J, Montano L, Maestre J, et al. Multiplex polymerase chain reaction amplification and differentiation of Entamoeba histolytica and Entatneoba. dispar DNA from stool samples. Am J Trop Med Hyg 2001; 64:293-7.
10. Ramos F, Morán P, González E, García G, Ramiro M, Gómez A, et al. Entamoeba- histolytica and Entamoeba dispar. prevalence infection in a rural Mexican community. Exp Parasitol 2005; 110: 327-30.
11. Rivera W, Tachibana H, Kanbara H. Field study on the distribution of Entamoeba histolytica and Entamoeba dispar in the Northern Philippines as detected by the polymerase chain reaction. Am J Trop Med Hyg 1998; 59:916-21.
12. Stanley L, Blanchard L, Johnson N, Foster L, Kunz- Jenkins C, Zhang T, et al. Immunogenicity of the recombinant Serine Rich Entamoeba histolytica Protein (SREHP) amebiasis vaccine in the African green monkey. Vaccine 1995; 13:947-51.
13. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. ] Mol Biol 1990;215:403-10.
14. Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, et al. Gapped BLAST and PSI- BLAST: a new generation of protein database search programs. Nucleic Acids Res 1997; 25: 3389-402.
15. Blessmann J, Buss H, Ton Un P, Dinh B, Viet Ngo Q, Le Van A, et al. Real-time PCR for detection and differentiation of Entamoeba histolytica and Entamoeba dispar in fecal samples. J Clin Microbiol 2002; 40:4413-7.
16. Daniel W. Bioestadística. Base para el analisis de las ciencias de la salud. 4a Ed. México DF: Limusa Wiley; 2002.
17. Rivero Z, Díaz I, Acurero E, Camacho MC, Medina M, Ríos L. Prevalencia de Parásitos Intestinales en Escolares de 5 a 10 años de un Instituto del Municipio Maracaibo. Estado Zulia. Kasmera 2001; 29:153-70.
18. Díaz I, Rivero Z, Bracho A, Castellanos M, Acurero E, Calchi M, et al. Prevalencia de enteroparásitos en niños de la etnia Yukpa de Toromo, Estado Zulia, Venezuela. Rev Méd Chil 2006; 134:72-8.
19. Chacín-Bonilla L, Dikdan Y. Prevalencia de Entamoeba histolytica y otros parásitos intestinales en una comunidad suburbana de Maracaibo. Invest Clin 1981; 22:185-203.
20. World Health Organization. Amoebiasis. Wkly Epidemiol Rec 1997; 72:97-100.
21. Evangelopoulos A, Legakis N, Vakalis N. Microscopy, PCR and ELISA applied to the epidemiology of amoebiasis in Greece. Parasitol Int 2001; 50:185-9.
22. Troll H, Marti H, Weiss N. Simple differential detection of Entamoeba histolytica y Entamoeba- dispar in fresh stool specimens by sodium acetateacetc acid-formalin concentration and PCR. J Clin Microbiol 1997; 35:1701-5.
23. Morán R Ramos F, Ramiro M, Curiel O, Gonzalez E, Valadez A, et al. Entamoeba histolytica and/or Entamoeba dispar. infection frequency in HIV/AIDS patients in Mexico City. Exp Parasitol 2005; 110:331-4.
24. el-Hamshary EM, el-Shewy KA, Hezagy MM, Zakaria H. Selective identification of the pathogenic E. histolytica in fresh stool simples using polymerase chain reaction (PCR). J Egypt Soc Parasitol 2004;34:611-20.
25. Gonin P, Trudel L. Detection and differentiation of Entamoeba histolytica and Entamoeba dispar isolates in clinical samples by PCR and enzymelinked immunosorbent assay. J Clin Microbiol 2003;41:237-41.
26. Pinheiro SM, Carneiro RM, Aca IS, Irmao JI, Morais MA, Coimbra MR, et al. Determination of the prevalence of Entamoeba histolytica and E. dispar in the Pernambuco State of Northeastern Brazil by a polymerase chain reaction. Am J Trop Med Hyg 2004; 70:221-4.
27. Calderaro A, Gorrini Ch, Bommezzadri S, Piccolo G, Dettori G, Chezzi C. Entamoeba histolytica and Entamoeba dispar. comparison of two PCR assays for diagnosis in a non-endemic setting. Trans R Soc Trop Med Hyg 2006; 100:450-7.
28. Visser L, Verweij J, Van M, EdelingW, Clreinx I, Polderman A. Diagnostic methods for differentiation of Entamoeba histolytica and Entamoeba dispar in carries: performance and clinical implications in a non-endemic setting. Int I Med Microbiol 2006; 296:397-403.
29. Verweij J, Oostvogel F, Brienen E, Nang-Beifubah A, Ziem J, Polderman A. Prevalence of Entamoeba histolytica and Entamoeba dispar in northern Ghana. Trop Med Int Health 2003; 8:1153-6.
30. Walderich B, Weber A, Knobloch J. Differentiation of Entamoeba histolytica and Entamoeba dispar from German travelers and residents of endemic areas. Am J Trop Hyg 1997; 57:70-4.
31. Mora L, García A, De Donato M, Urdaneta H. Estudio epidemiológico y molecular de Entamoeba histolytica y Entamoeba dispar en pacientes con diarrea en Cumaná, Estado Sucre, Venezuela. Invest Clin 2008; en prensa.
32. Sánchez-Guillén M, Pérez-Fuentes R, Salgado-Rosas H, Ruiz-Argüelles A, Ackers J, Shire A, et al. Differentiation of Entamoeba histolytica/Entamoeba dispar by PCR and their correlation with humoral and cellular immunity in individuals with clinical variants of amoebiasis. Am I Trop Med Hyg 2002; 66:731-7.
33. Mora L, García A, De Donato M. Prevalencia del complejo Entamoeba histolytica/Entamoeba dispar en pacientes con síntomas gastrointestinales de diarrea procedentes de Cumaná, Estado Sucre. Kasmera 2005; 33:36-45.
34. Póvoa MM, Arruda JEG, Silva MCM, Bichara CNC, Esteves P, Gabbay YB, et al. Diagnóstico de amebí- ase intestinal utilizando métodos coproscópicos e imunológicos em amostra da população da $Aarea metropolitana de Belém, Pará, Brasil. Cad Saúde Publica2000; 16:843-6.
35. Silva MCM, Monteiro CSP, Araújo BAV Sillva JV Pó- voa MM. Determinação da infecção por Entamoeba histolytica em residentes da área metropolitana de Belém, Pará, Brazil, utilizando ensaio imunoenzimático (ELISA) para detecção de antígenos. Cad Saúde Publica 2005; 21:969-73.
36. Dirección de Informatión Social y Estadísticas, Directión General de Epidemiología, Ministerio de Salud. Anuario de mortalidad 2005. Caracas: Ministerio de Salud; 2006.
37. Masalán M, González R. Autocuidado del ciclo vital. http://www.puc.cl/sw-educ/enferm/ciclo/index. html (accedido el Dic/2003).