Overexpression, purification and crystallization of a thermostable DNA ligase from the archaeon Thermococcus sp. 1519

Acta Crystallographica Section F: Structural Biology and Crystallization Communications

Volumn 65, Issue 4, 2009, Pages 368-371

  Bezsudnova, E Y ^a   Kovalchuk, M V ^b,c   Mardanov, A V ^d   Poliakov, K M ^e   Popov, V O ^a   Ravin, N V ^d   Skryabin, K G ^d   Smagin, V A ^d   Stekhanova, T N ^a   Tikhonova, T V ^a  

^a BACH INSTITUTE OF BIOCHEMISTRY   (Russian Federation)

^b SHUBNIKOV INSTITUTE OF CRYSTALLOGRAPHY   (Russian Federation)

^c KURCHATOV INSTITUTE   (Russian Federation)

^d INSTITUTE OF BIOENGINEERING   (Russian Federation)

^e ENGELHARDT INSTITUTE OF MOLECULAR BIOLOGY   (Russian Federation)

Author keywords

Archaea; DNA ligases

Indexed keywords

POLYDEOXYRIBONUCLEOTIDE SYNTHASE; RECOMBINANT PROTEIN;

ARTICLE; CHEMISTRY; CRYSTALLIZATION; ENZYME STABILITY; ENZYMOLOGY; ISOLATION AND PURIFICATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; TEMPERATURE; THERMOCOCCUS; X RAY CRYSTALLOGRAPHY;

CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; DNA LIGASES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME STABILITY; RECOMBINANT PROTEINS; TEMPERATURE; THERMOCOCCUS;

ARCHAEA; THERMOCOCCUS; THERMOCOCCUS SP.;

EID: 64149121656     PISSN: 17443091     EISSN: 17443091     Source Type: Journal    
DOI: 10.1107/S1744309109007799     Document Type: Article

References (15)

1. Collaborative Computational Project, Number 4 (1994). Acta Cryst. D50, 760-763.
2. Kabsch, W. (1993). J. Appl. Cryst. 26, 795-800.
3. Keppetipola, N. & Shuman, S. (2005). J. Bacteriol. 187, 6902-6908.
4. Lai, X., Shao, H., Hao, F. & Huang, L. (2002). Extremophiles, 6, 469-477.
5. Lehman, I. R. (1974). Science, 186, 790-797.
6. Nakatani, M., Ezaki, S., Atomi, H. & Imanaka, T. (2000). J. Bacteriol. 182, 6424-6433.
7. Nishida, H., Kiyonari, S., Ishino, Y. & Morikawa, K. (2006). J. Mol. Biol. 360, 956-967.
8. Pascal, J. M. (2008). Curr. Opin. Struct. Biol. 18, 96-105.
9. Pascal, J. M., O'Brien, P. J., Tomkinson, A. E. & Ellenberger, T. (2004). Nature (London), 432, 473-478.
10. Pascal, J. M., Tsodikov, O. V., Hura, G. L., Song, W., Cotner, E. A., Classen, S., Tomkinson, A. E., Tainer, J. A. & Ellenberger, T. (2006). Mol. Cell, 24, 279-291.
11. Rolland, J., Gueguen, Y., Persillon, C., Masson, J. & Dietrich, J. (2004). FEMS Microbiol. Lett. 236, 267-273.
12. Smagin, V. A., Mardanov, A. V., Bonch-Osmolovkaya, E. A. & Ravin, N. V. (2008). Prikl. Biokhim. Mikrobiol. 44, 523-528.
13. Sriskanda, V., Kelman, Z., Hurwitz, J. & Shuman, S. (2000). Nucleic Acids Res. 28, 2221-2228.
14. Vagin, A. A., Steiner, R. A., Lebedev, A. A., Potterton, L., McNicholas, S., Long, F. & Murshudov, G. N. (2004). Acta Cryst. D60, 2184-2195.
15. Vagin, A. & Teplyakov, A. (1997). J. Appl. Cryst. 30, 1022-1025.