In Vitro Characterization of the Enzyme Properties of the Phospholipid N-Methyltransferase PmtA from Agrobacterium tumefaciens

Journal of Bacteriology

Volumn 191, Issue 7, 2009, Pages 2033-2041

  Aktas, Meriyem ^a   Narberhaus, Franz ^a  

^a RUHR UNIVERSITY BOCHUM   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ENZYME; METHYLTRANSFERASE; PHOSPHATIDYLCHOLINE; PHOSPHATIDYLETHANOLAMINE; PHOSPHATIDYLGLYCEROL; PHOSPHATIDYLINOSITOL; PHOSPHOLIPID; PMTA ENZYME; S ADENOSYLHOMOCYSTEINE; S ADENOSYLMETHIONINE; SINEFUNGIN; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; PMTA PROTEIN, BACTERIA;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME BINDING; ENZYME INHIBITION; ENZYME PURIFICATION; ENZYME SUBSTRATE; IN VITRO STUDY; NONHUMAN; PRIORITY JOURNAL; RHIZOBIUM RADIOBACTER; CHEMISTRY; ENZYME SPECIFICITY; ENZYMOLOGY; GENETICS; ISOLATION AND PURIFICATION; METABOLISM;

AGROBACTERIUM TUMEFACIENS; BACTERIA (MICROORGANISMS); PHOS; PROKARYOTA;

BACTERIAL PROTEINS; METHYLTRANSFERASES; PHOSPHOLIPIDS; RHIZOBIUM RADIOBACTER; S-ADENOSYLHOMOCYSTEINE; SUBSTRATE SPECIFICITY;

EID: 64049114347     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.01591-08     Document Type: Article

References (44)

1. Arondel, V., C. Benning, and C. R. Somerville. 1993. Isolation and functional expression in Escherichia coli of a gene encoding phosphatidylethanolamine methyltransferase (EC 2.1.1.17) from Rhodobacter sphaeroides. J. Biol. Chem. 268:16002-16008.
2. Bligh, E. G., and W. J. Dyer. 1959. A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37:911-917.
3. Boeck, L. D., G. M. Clem, M. M. Wilson, and J. E. Westhead. 1973. A9145, a new adenine-containing antifungal antibiotic: fermentation. Antimicrob. Agents Chemother. 3:49-56.
4. Carman, G. M., R. A. Deems, and E. A. Dennis. 1995. Lipid signaling enzymes and surface dilution kinetics. J. Biol. Chem. 270:18711-18714.
5. Cho, W., and R. V. Stahelin. 2005. Membrane-protein interactions in cell signaling and membrane trafficking. Annu. Rev. Biophys. Biomol. Struct. 34:119-151.
6. Comerci, D. J., S. Altabe, D. de Mendoza, and R. A. Ugalde. 2006. Brucella abortus synthesizes phosphatidylcholine from choline provided by the host. J. Bacteriol. 188:1929-1934.
7. Conde-Alvarez, R., M. J. Grillo', S. P. Salcedo, M. J. de Miguel, E. Fugier, J. P. Gorvel, I. Moriyo'n, and M. Iriarte. 2006. Synthesis of phosphatidylcholine, a typical eukaryotic phospholipid, is necessary for full virulence of the intracellular bacterial parasite Brucella abortus. Cell. Microbiol. 8:1322- 1335.
8. Conover, G. M., F. Martínez-Morales, M. I. Heidtman, Z. Q. Luo, M. Tang, C. Chen, O. Geiger, and R. R. Isberg. 2008. Phosphatidylcholine synthesis is required for optimal function of Legionella pneumophila virulence determinants. Cell. Microbiol. 10:514-528.
9. Cui, Z., J. E. Vance, M. H. Chen, D. R. Voelker, and D. E. Vance. 1993. Cloning and expression of a novel phosphatidylethanolamine N-methyltransferase. A specific biochemical and cytological marker for a unique membrane fraction in rat liver. J. Biol. Chem. 268:16655-16663.
10. de Rudder, K. E., I. M. Lo'pez-Lara, and O. Geiger. 2000. Inactivation of the gene for phospholipid N-methyltransferase in Sinorhizobium meliloti: phosphatidylcholine is required for normal growth. Mol. Microbiol. 37: 763-772.
11. Dorgan, K. M., W. L. Wooderchak, D. P. Wynn, E. L. Karschner, J. F. Alfaro, Y. Cui, Z. S. Zhou, and J. M. Hevel. 2006. An enzyme-coupled continuous spectrophotometric assay for S-adenosylmethionine-dependent methyltransferases. Anal. Biochem. 350:249-255.
12. Dowler, S., G. Kular, and D. R. Alessi. 2002. Protein lipid overlay assay. Sci. STKE 2002(129):PL6.
13. Gaynor, P. M., and G. M. Carman. 1990. Phosphatidylethanolamine methyltransferase and phospholipid methyltransferase activities from Saccharomyces cerevisiae. Enzymological and kinetic properties. Biochim. Biophys. Acta 1045:156-163.
14. Gibson, K. D., J. D. Wilson, and S. Udenfriend. 1961. The enzymatic conversion of phospholipid ethanolamine to phospholipid choline in rat liver. J. Biol. Chem. 236:673-679.
15. Goldfine, H. 1984. Bacterial membranes and lipid packing theory. J. Lipid Res. 25:1501-1507.
16. Hacker, S., J. Gödeke, A. Lindemann, S. Mesa, G. Pessi, and F. Narberhaus. 2008. Global consequences of phosphatidylcholine reduction in Bradyrhizobium japonicum. Mol. Genet. Genomics 280:59-72.
17. Hacker, S., C. Sohlenkamp, M. Aktas, O. Geiger, and F. Narberhaus. 2008. Multiple phospholipid N-methyltransferases with distinct substrate specificities are encoded in Bradyrhizobium japonicum. J. Bacteriol. 190:571-580.
18. Haydock, S. F., J. A. Dowson, N. Dhillon, G. A. Roberts, J. Cortes, and P. F. Leadlay. 1991. Cloning and sequence analysis of genes involved in erythromycin biosynthesis in Saccharopolyspora erythraea: sequence similarities between EryG and a family of S-adenosylmethionine-dependent methyltransferases. Mol. Gen. Genet. 230:120-128.
19. Ingrosso, D., A. V. Fowler, J. Bleibaum, and S. Clarke. 1989. Sequence of the D-aspartyl/L-isoaspartyl protein methyltransferase from human erythrocytes. Common sequence motifs for protein, DNA, RNA, and small molecule S-adenosylmethionine-dependent methyltransferases. J. Biol. Chem. 264: 20131-20139.
20. Kaneshiro, T., and J. H. Law. 1964. Phosphatidylcholine synthesis in Agrobacterium tumefaciens. I. Purification and properties of a phosphatidylethanolamine N-methyltransferase. J. Biol. Chem. 239:1705-1713.
21. Kanipes, M. I., and S. A. Henry. 1997. The phospholipid methyltransferases in yeast. Biochim. Biophys. Acta 1348:134-141.
22. + gene encodes a phospholipid methyltransferase. Genetics 150: 553-562.
23. Karnezis, T., H. C. Fisher, G. M. Neumann, B. A. Stone, and V. A. Stanisich. 2002. Cloning and characterization of the phosphatidylserine synthase gene of Agrobacterium sp. strain ATCC 31749 and effect of its inactivation on production of high-molecular-mass (133)-ß-D-glucan (curdlan). J. Bacteriol. 184:4114-4123.
24. Klüsener, S., M. Aktas, K. M. Thormann, M. Wessel, and F. Narberhaus. 2009. Expression and physiological relevance of Agrobacterium tumefaciensphosphatidylcholine biosynthesis genes. J. Bacteriol. 191:365-374.
25. Kodaki, T., and S. Yamashita. 1987. Yeast phosphatidylethanolamine methylation pathway. Cloning and characterization of two distinct methyltransferase genes. J. Biol. Chem. 262:15428-15435.
26. Lo'pez-Lara, I. M., and O. Geiger. 2001. Novel pathway for phosphatidylcholine biosynthesis in bacteria associated with eukaryotes. J. Biotechnol. 91:211-221.
27. Lo'pez-Lara, I. M., C. Sohlenkamp, and O. Geiger. 2003. Membrane lipids in plant-associated bacteria: their biosyntheses and possible functions. Mol. Plant-Microbe Interact. 16:567-579.
28. Martin, J. L., and F. M. McMillan. 2002. SAM (dependent) I AM: the S-adenosylmethionine-dependent methyltransferase fold. Curr. Opin. Struct. Biol. 12:783-793.
29. Martínez-Morales, F., M. Schobert, I. M. Lo'pez-Lara, and O. Geiger. 2003. Pathways for phosphatidylcholine biosynthesis in bacteria. Microbiology 149: 3461-3471.
30. Minder, A. C., K. E. de Rudder, F. Narberhaus, H. M. Fischer, H. Hennecke, and O. Geiger. 2001. Phosphatidylcholine levels in Bradyrhizobium japonicummembranes are critical for an efficient symbiosis with the soybean host plant. Mol. Microbiol. 39:1186-1198.
31. Pessi, G., G. Kociubinski, and C. B. Mamoun. 2004. A pathway for phosphatidylcholine biosynthesis in Plasmodium falciparum involving phosphoethanolamine methylation. Proc. Natl. Acad. Sci. USA 101:6206- 6211.
32. Ridgway, N. D., and D. E. Vance. 1988. Kinetic mechanism of phosphatidylethanolamine N-methyltransferase. J. Biol. Chem. 263:16864-16871.
33. Ridgway, N. D., and D. E. Vance. 1987. Purification of phosphatidylethanolamine N-methyltransferase from rat liver. J. Biol. Chem. 262:17231-17239.
34. Sherr, S. I., and J. H. Law. 1965. Phosphatidylcholine synthesis in Agrobacterium tumefaciens. II. Uptake and utilization of choline. J. Biol. Chem. 240:3760-3765.
35. Shields, D. J., J. Y. Altarejos, X. Wang, L. B. Agellon, and D. E. Vance. 2003. Molecular dissection of the S-adenosylmethionine-binding site of phosphatidylethanolamine N-methyltransferase. J. Biol. Chem. 278:35826-35836.
36. Shields, D. J., S. Lingrell, L. B. Agellon, J. T. Brosnan, and D. E. Vance. 2005. Localization-independent regulation of homocysteine secretion by phosphatidylethanolamine N-methyltransferase. J. Biol. Chem. 280:27339- 27344.
37. Sohlenkamp, C., I. M. Lo'pez-Lara, and O. Geiger. 2003. Biosynthesis of phosphatidylcholine in bacteria. Prog. Lipid Res. 42:115-162.
38. Tahara, Y., Y. Ogawa, T. Sakakibara, and Y. Yamada. 1987. Purification and characterization of phosphatidylethanolamine N-methyltransferase from Zymomonas mobilis. Agric. Biol. Chem. 51:1425-1430.
39. Vance, D. E., Z. Li, and R. L. Jacobs. 2007. Hepatic phosphatidylethanol amine N-methyltransferase, unexpected roles in animal biochemistry and physiology. J. Biol. Chem. 282:33237-33241.
40. Vance, J. E. 1990. Phospholipid synthesis in a membrane fraction associated with mitochondria. J. Biol. Chem. 265:7248-7256.
41. Walkey, C. J., L. R. Donohue, R. Bronson, L. B. Agellon, and D. E. Vance. 1997. Disruption of the murine gene encoding phosphatidylethanolamine N-methyltransferase. Proc. Natl. Acad. Sci. USA 94:12880-12885.
42. Wessel, M., S. Klüsener, J. Gödeke, C. Fritz, S. Hacker, and F. Narberhaus. 2006. Virulence of Agrobacterium tumefaciens requires phosphatidylcholine in the bacterial membrane. Mol. Microbiol. 62:906-9015.
43. Wilderman, P. J., A. I. Vasil, W. E. Martin, R. C. Murphy, and M. L. Vasil. 2002. Pseudomonas aeruginosa synthesizes phosphatidylcholine by use of the phosphatidylcholine synthase pathway. J. Bacteriol. 184:4792-4799.
44. Zhu, Y., C. Qi, W. Q. Cao, A. V. Yeldandi, M. S. Rao, and J. K. Reddy. 2001. Cloning and characterization of PIMT, a protein with a methyltransferase domain, which interacts with and enhances nuclear receptor coactivator PRIP function. Proc. Natl. Acad. Sci. USA 98:10380-10385.