Brucella abortus synthesizes phosphatidylcholine from choline provided by the host

Journal of Bacteriology

Volumn 188, Issue 5, 2006, Pages 1929-1934

  Comerci, Diego J ^a,b   Altabe, Silvia ^c   De Mendoza, Diego ^c   Ugalde, Rodolfo A ^a,b  

^a INSTITUTO DE INVESTIGACIONES BIOTECNOLÓGICAS   (Argentina)

^b CENTRO ATÓMICO EZEIZA   (Argentina)

^c UNIVERSIDAD NACIONAL DE ROSARIO   (Argentina)

Author keywords

[No Author keywords available]

Indexed keywords

CHOLINE; ISOPROPYL BETA THIOGALACTOSIDE; PHOSPHATIDYLCHOLINE; PHOSPHATIDYLCHOLINE SYNTHASE; PHOSPHOLIPID; PYRANOSIDE; SYNTHETASE; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BACTERIAL GENE; BACTERIAL GROWTH; BACTERIAL MEMBRANE; BACTERIAL VIRULENCE; BACTERIUM MUTANT; BACTERIUM TRANSFORMATION; BRUCELLA ABORTUS; CONTROLLED STUDY; CULTURE MEDIUM; ESCHERICHIA COLI; EXPRESSION VECTOR; FEMALE; HOST CELL; HOST PATHOGEN INTERACTION; INFECTION; LIPID COMPOSITION; MACROPHAGE; METHYLATION; MOUSE; NONHUMAN; PCS GENE; PHENOTYPE; PHOSPHOLIPID SYNTHESIS; PMTA GENE; PRIORITY JOURNAL; SEQUENCE HOMOLOGY; VEGETATIVE GROWTH;

AMINO ACID SEQUENCE; ANIMALS; BACTERIAL PROTEINS; BRUCELLA ABORTUS; BRUCELLOSIS; CHOLINE; ESCHERICHIA COLI; FEMALE; METHYLTRANSFERASES; MICE; MICE, INBRED BALB C; MOLECULAR SEQUENCE DATA; MUTATION; PHOSPHATIDYLCHOLINES; SEQUENCE ALIGNMENT; TRANSFERASES (OTHER SUBSTITUTED PHOSPHATE GROUPS); VIRULENCE;

ANIMALIA; BACTERIA (MICROORGANISMS); BRUCELLA; BRUCELLA MELITENSIS BIOVAR ABORTUS; ESCHERICHIA COLI; EUKARYOTA; PROKARYOTA;

EID: 33644772849     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.188.5.1929-1934.2006     Document Type: Article

References (24)

1. Bligh, E. G., and W. J. Dyer. 1959. A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37:911-917.
2. Casao, M. A., R. Diaz, A. Orduna, and C. Gamazo. 2001. Promotion of platelet aggregation by sera from brucellosis patients with antiphosphatidylcholine antibodies. J. Med. Microbiol. 50:965-968.
3. Casao, M. A., J. Leiva, R. Diaz, and C. Gamazo. 1998. Anti-phosphatidylcholine antibodies in patients with brucellosis. J. Med. Microbiol. 47:49-54.
4. Celli, J., and J. P. Gorvel. 2004. Organelle robbery: Brucella interactions with the endoplasmic reticulum. Curr. Opin. Microbiol. 7:93-97.
5. Comerci, D. J., G. D. Pollevick, A. M. Vigliocco, A. C. Frasch, and R. A. Ugalde. 1998. Vector development for the expression of foreign proteins in the vaccine strain Brucella abortus S19. Infect. Immun. 66:3862-3866.
6. Corbel, M. J. 1997. Brucellosis: an overview. Emerg. Infect. Dis. 3:213-221.
7. de Rudder, K. E., I. M. Lopez-Lara, and O. Geiger. 2000. Inactivation of the gene for phospholipid N-methyltransferase in Sinorhizobium meliloti: phosphatidylcholine is required for normal growth. Mol. Microbiol. 37:763-772.
8. de Rudder, K. E., C. Sohlenkamp, and O. Geiger. 1999. Plant-exuded choline is used for rhizobial membrane lipid biosynthesis by phosphatidylcholine synthase. J. Biol. Chem. 274:20011-20016.
9. de Rudder, K. E., J. E. Thomas-Oates, and O. Geiger. 1997. Rhizobium meliloti mutants deficient in phospholipid N-methyltransferase still contain phosphatidylcholine. J. Bacteriol. 179:6921-6928.
10. Gerhardt, P. 1958. The nutrition of brucellae. Bacteriol. Rev. 22:81-98.
11. Kent, C., P. Gee, S. Y. Lee, X. Bian, and J. C. Fenno. 2004. A CDP-choline pathway for phosphatidylcholine biosynthesis in Treponema denticola. Mol. Microbiol. 51:471-481.
12. Lysenko, E., J. C. Richards, A. D. Cox, A. Stewart, A. Martin, M. Kapoor, and J. N. Weiser. 2000. The position of phosphorylcholine on the lipopolysaccharide of Haemophilus influenzae affects binding and sensitivity to C-reactive protein-mediated killing. Mol. Microbiol. 35:234-245.
13. Martinez-Morales, F., M. Schobert, I. M. Lopez-Lara, and O. Geiger. 2003. Pathways for phosphatidylcholine biosynthesis in bacteria. Microbiology 149:3461-3471.
14. Minder, A. C., K. E. de Rudder, F. Narberhaus, H. M. Fischer, H. Hennecke, and O. Geiger. 2001. Phosphatidylcholine levels in Bradyrhizobium japonicum membranes are critical for an efficient symbiosis with the soybean host plant. Mol. Microbiol. 39:1186-1198.
15. Moriyon, I., and I. Lopez-Goni. 1998. Structure and properties of the outer membranes of Brucella abortus and Brucella melitensis. Int. Microbiol. 1:19-26.
16. Prescott, S. M., G. A. Zimmerman, D. M. Stafforini, and T. M. McIntyre. 2000. Platelet-activating factor and related lipid mediators. Annu. Rev. Biochem. 69:419-445.
17. Sohlenkamp, C., K. E. de Rudder, V. V. Rohrs, I. M. Lopez-Lara, and O. Geiger. 2000. Cloning and characterization of the gene for phosphatidylcholine synthase. J. Biol. Chem. 275:27500.
18. Sohlenkamp, C., I. M. Lopez-Lara, and O. Geiger. 2003. Biosynthesis of phosphatidylcholine in bacteria. Prog. Lipid Res. 42:115-162.
19. Thiele, O. W., J. Asselineau, and C. Lacave. 1969. On the fatty acids of Brucella abortus and Brucella melitensis. Eur. J. Biochem. 7:393-396.
20. Thiele, O. W., and W. Kehr. 1969. The "free" lipids of Brucella abortus Bang. Concerning the neutral lipids. Eur. J. Biochem. 9:167-175. [In German.]
21. Walterscheid, J. P., S. E. Ullrich, and D. X. Nghiem. 2002. Platelet-activating factor, a molecular sensor for cellular damage, activates systemic immune suppression. J. Exp. Med. 195:171-179.
22. Welsh, C. J., and K. Schmeichel. 1991. Assays for investigations of signal transduction mechanisms involving phospholipase D: mass measurements of phosphatidate, phosphatidylethanol, and diacylglycerol in cultured cells. Anal. Biochem. 192:281-292.
23. Wilderman, P. J., A. I. Vasil, W. E. Martin, R. C. Murphy, and M. L. Vasil. 2002. Pseudomonas aeruginosa synthesizes phosphatidylcholine by use of the phosphatidylcholine synthase pathway. J. Bacteriol. 184:4792-4799.
24. Zhang, J. R., I. Idanpaan-Heikkila, W. Fischer, and E. I. Tuomanen. 1999. Pneumococcal licD2 gene is involved in phosphorylcholine metabolism. Mol. Microbiol. 31:1477-1488.