HRMAS 1H-NMR measured changes of the metabolite profile as mesenchymal stem cells differentiate to targeted fat cells in vitro: Implications for non-invasive monitoring of stem cell differentiation in vivo

Journal of Tissue Engineering and Regenerative Medicine

Volumn 2, Issue 8, 2008, Pages 482-490

  Shi, Chunmeng ^a,b   Wang, Xiaoxia ^b   Wu, Shaoxiong ^c   Zhu, Ying ^a,b   Chung, Leland W K ^b   Mao, Hui ^b  

^a THIRD MILITARY MEDICAL UNIVERSITY   (China)

^b EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c EMORY UNIVERSITY   (United States)

Author keywords

Adipogenic differentiation; Imaging; Magnetic resonance; Metabolite; Spectroscopy; Stem cell

Indexed keywords

ALCOHOLS; BIOMOLECULES; CELL CULTURE; FATTY ACIDS; MAGIC ANGLE SPINNING; MAGNETISM; METABOLISM; METABOLITES; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; POLYOLS; RATS;

ADIPOGENIC DIFFERENTIATIONS; CELL-BE; CELL/B.E; CELL/BE; IN-VITRO; IN-VIVO; MESENCHYMAL STEM CELL; METABOLITE; MYO-INOSITOL; STEM-CELL;

STEM CELLS;

CHOLINE; CREATINE; FATTY ACID; GLUTAMIC ACID; INOSITOL; AZO COMPOUND; OIL RED O;

ADIPOCYTE; ANIMAL CELL; ARTICLE; CELL DIFFERENTIATION; MESENCHYMAL STEM CELL; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTON NUCLEAR MAGNETIC RESONANCE; ADIPOGENESIS; ANIMAL; CYTOLOGY; METABOLISM; METABOLOME; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY;

ADIPOCYTES; ADIPOGENESIS; ANIMALS; AZO COMPOUNDS; CELL DIFFERENTIATION; MAGNETIC RESONANCE SPECTROSCOPY; MESENCHYMAL STEM CELLS; METABOLOME; MICE;

EID: 64049085457     PISSN: 19326254     EISSN: 19327005     Source Type: Journal    
DOI: 10.1002/term.120     Document Type: Article

References (34)

1. Barak NMC, Ong ES, Jones YZ, et al. 1999; PPARγ is required for placental, cardiac, and adipose tissue development. Mol Cell 4: 585-595.
2. Chen CH, Ho ML, Chang JK, et al. 2005; Green tea catechin enhances osteogenesis in a bone marrow mesenchymal stem cell line. Osteoporos Int 16: 2039-2045.
3. Chen JH, Enloe BM, Weybright P, et al. 2002; Biochemical correlates of thiazolidinedione-induced adipocyte differentiation by high-resolution magic angle spinning NMR spectroscopy. Magn Reson Med 48: 602-610.
4. Cheng LL, Ma MJ, Becerra L, et al. 1997; Quantitative neuropathology by high resolution magic angle spinning proton magnetic resonance spectroscopy. Proc Natl Acad Sci USA 94: 6408-6413. (Pubitemid 27270698)
5. Cheng LL, Newella K, Mallorya AE, et al. 2002; Quantification of neurons in Alzheimer and control brains with ex vivo high resolution magic angle spinning proton magnetic resonance spectroscopy and stereology. Magn Reson Imaging 20: 527-533.
6. 18F] fluorodopa/PET and histology of fetal mesencephalic dopaminergic grafts to the striatum of MPTP-poisoned minipigs. Cell Transpl 11: 733-746.
7. Englund U, Bjorklund A, Wictorin K, et al. 2002; Grafted neural stem cells develop into functional pyramidal neurons and integrate into host cortical circuitry. Proc Natl Acad Sci USA 99: 17089-17094. (Pubitemid 36034103)
8. Frangioni JV, Hajjar RJ. 2004; In vivo tracking of stem cells for clinical trials in cardiovascular disease. Circulation 110: 3378-3383. (Pubitemid 39557503)
9. Friedmann T. 2005; Lessons for the stem cell discourse from the gene therapy experience. Perspect Biol Med 48: 585-591. (Pubitemid 41464112)
10. 1H-NMR spectroscopy. NMR Biomed 15: 375-384.
11. 1H-NMR spectroscopy. Magn Reson Med 46: 249-255.
12. Janderova L, McNeil M, Murrell AN, et al. 2003; Human mesenchymal stem cells as an in vitro model for human adipogenesis. Obesity Res 11: 65-74. (Pubitemid 41137540)
13. Jansen JF, Shamblott MJ, van Zijl PC, et al. 2006; Stem cell profiling by nuclear magnetic resonance spectroscopy. Magn Reson Med 56: 666-670. (Pubitemid 44338887)
14. 31P NMR of PCA extracts of postmortem Alzheimer's disease brain. Neurobiol Aging 17: 349-357.
15. Lewin M, Cariesso N, Tung CH, et al. 2000; Tat peptide-derivatized magnetic nanoparticles allow in vivo tracking and recovery of progenitor cells. Nat Biotechnol 18: 410-414. (Pubitemid 30217528)
16. Li W. 2006; Multidimensional HRMAS NMR: a platform for in vivo studies using intact bacterial cells. Analyst 131: 777-781. (Pubitemid 43962639)
17. Mao H, Wang X, Lacreuse A, et al. 2007; HRMAS NMR measured metabolic changes in kainic acid-induced hippocampal injury in rat. Exp Brain Res 183: 477-485. (Pubitemid 350029364)
18. 1H-NMR study of human high grade gliomas. NMR Biomed 17: 191-205.
19. Martinez-Granados B, Monleon D, Martinez-Bisbal MC, et al. 2006; Metabolite identification in human liver needle biopsies by high-resolution magic angle spinning 1H-NMR spectroscopy. NMR Biomed 19: 90-100.
20. Manganas LN, Zhang X, Li Y, et al. 2007; Magnetic resonance spectroscopy identifies neural progenitor cells in the live human brain. Science 318: 980-985.
21. Nagayama K, Kumar K, Wuthrich K, et al. 1980; Experimental techniques of two-dimensional correlated spectroscopy. J Magn Reson 40: 321-334.
22. Nuttall ME, Gimble JM. 2000; Is there a therapeutic opportunity to either prevent or treat osteopenic disorders by inhibiting marrow adipogenesis? Bone 27: 177-184.
23. Pittenger MF, Mackay AM, Beck SC, et al. 1999; Multilineage potential of adult human mesenchymal stem cells. Science 284: 143-147.
24. Pogarell O, Koch W, Gildehaus FJ, et al. 2006; Long-term assessment of striatal dopamine transporters in Parkinsonian patients with intrastriatal embryonic mesencephalic grafts. Eur J Nucl Med Mol Imaging 33: 407-411.
25. 1H spectroscopy. Glutamate and glutamine; myo-inositol and related metabolites. NMR Biomed 4: 59-63.
26. Ross BD, Bluml S, Cowan R. 1998; In vivo MR spectroscopy of human dementia. Neuroimag Clin N Am 8: 809-822.
27. Ross BD, Bluml S, Cowan R, et al. 1997; In vivo magnetic resonance spectroscopy of human brain: the biophysical basis of dementia. Biophys Chem 68: 161-172. (Pubitemid 28064663)
28. Schilling T, Noth U, Klein-Hitpass L, et al. 2007; Plasticity in adipogenesis and osteogenesis of human mesenchymal stem cells. Mol Cell Endocrinol 271: 1-17. (Pubitemid 46803047)
29. Shyu WC, Chen CP, Lin SZ, et al. 2007; Efficient tracking of non-iron-labelled mesenchymal stem cells with serial MRI in chronic stroke rats. Stroke 38: 367-374.
30. Singer S, Sivaranja M, Souza K, et al. 1996; 1H-NMR detectable fatty acyl chain unsaturation in excised leiomyosarcoma correlate with grade and mitotic activity. J Clin Invest 98: 244-250. (Pubitemid 26255719)
31. Swanson MG, Vigneron DB, Tabatabai ZL, et al. 2003; Proton HR-MAS spectroscopy and quantitative pathologic analysis of MRI/3D-NMRI-targeted postsurgical prostate tissues. Magn Reson Med 50: 944-954.
32. Szczepaniak LS, Babcock EE, Schick F, et al. 1999; Measurement of intracellular triglyceride stores by 1H spectroscopy: validation in vivo. Am J Physiol 276: 977-989.
33. Tong Z, Yamaki Y, Harada K, et al. 2004; In vivo quantification of the metabolites in normal brain and brain tumors by proton MR spectroscopy using water as an internal standard. Magn Reson Imaging 22: 1017-1024. (Pubitemid 38993532)
34. Trayhurn P, Beattie JH. 2001; Physiological role of adipose tissue: white adipose tissue as an endocrine and secretory organ. Proc Nutr Soc 60: 329-339. (Pubitemid 32772448)