SCOPUS 정보 검색 플랫폼

Synlett

Volumn , Issue 6, 2009, Pages 910-912

Practical phosphorylation methods for α,α-disubstituted α-amino alcohol derivatives

(5) Ohnuki, Takashi a Tsuji, Takashi a Miyazaki, Shojiro a Moriguchi, Taku a Nishi, Takahide a

a DAIICHI SANKYO CO (Japan)

Author keywords

Amino alcohols; Bioorganic chemistry; Biotransfomation; Phosphorylation; S1P1 receptor agonist

Indexed keywords

ALCOHOL DERIVATIVE; ALPHA AMINO ALCOHOL DERIVATIVE; AMINOALCOHOL; PHOSPHATE; UNCLASSIFIED DRUG;

ARTICLE; BIOTRANSFORMATION; CIRCINELLA MINOR; CIRCINELLA MUCOROIDES; CIRCINELLA MUSCAE; CIRCINELLA UMBELLATE; FUNGUS; NONHUMAN; PHOSPHORYLATION; PROTEIN SYNTHESIS;

EID: 63849153857 PISSN: 09365214 EISSN: 14372096 Source Type: Journal
DOI: 10.1055/s-0028-1087963 Document Type: Article

Times cited : (3)

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17
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- Typical Biotransformation Procedure Circinella muscae was cultivated at 23 °C for 2 d in Erlenmeyer flasks containing 80 mL of the medium consisting of 0.1% malt extract, 0.3% corn steep liquor, 1.0% glucose, 0.1% polypepton, 0.1% yeast extract, and 0.1% NaH2PO4 after seed cultivation at 23 °C for 2 d. To each of five Erlenmeyer flasks containing the fermentation broth, 0.8 mL of 8 (40 mg, l.lxl0 -4 mol) dissolved in 0.01% aq formic acid (4 mL) was added in order to start the biotransformation. The reaction was carried out at 23 °C for 3 d on a rotary shaker at 210 rpm. The fermentation broth (400 mL) was extracted with an equal of acetone with 40 μL of H3PO4, and the mixture was filtered. To the filtrate, an equal of distilled H2O was added, and then the mixture was adsorbed onto a column (40 mL) of DIAION HP-20 packed with 0.1, aq H3PO4. The colum
- -.

18
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- R = 6.7 and 3.2 min, respectively.
- R = 6.7 and 3.2 min, respectively.

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