Detection of E. coli O157:H7 experimentally inoculated in raw milk samples by conventional method and multiplex PCR;Detecção de Escherichia coli O157:H7 inoculada experimentalmente em amostras de leite cru por método convencional e PCR multiplex

Arquivo Brasileiro de Medicina Veterinaria e Zootecnia

Volumn 60, Issue 5, 2008, Pages 1241-1249

  Garcia, P M ^a   Arcuri, E F ^b   Brito, M A V P ^b   Lange, C C ^b   Brito, J R F ^b   Cerqueira, M M O P ^a  

^a FEDERAL UNIVERSITY OF MINAS GERAIS   (Brazil)

^b EMBRAPA CERRADOS   (Brazil)

Author keywords

E. coli O157:H7; H7 antigen; O157 antigen; PCR multiplex; Shiga like toxin genes

Indexed keywords

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 63649160164     PISSN: 01020935     EISSN: 16784162     Source Type: Journal    
DOI: 10.1590/S0102-09352008000500029     Document Type: Article

References (18)

1. Armstrong, G.L.; Hollingsworth, J.; Morris, G.J. JR. Emerging foodborne pathogens: Escherichia coli O157:H7 as a model of entry of a new pathogen into the food supply of the developed world. Epidemiol. Rev., v.18, p.29-51, 1996.
2. Borczyk, A.A.; Karmali, M.A.; Lior, H. et al. Bovine reservoir for verotoxin-producing Escherichia coli O157:H7. Lancet, v.30, p.98, 1987.
3. Bottero, M.T.; Dalmasso, A.; Soglia, D. et al. Development of a multiplex PCR assay for the identification of pathogenic genes of Escherichia coli in milk and milk products. Mol. Cell. Probes, v.18, p.283-288, 2004.
4. Cerqueira, A.M.; Guth, B.E.; Joaquim, R.M. et al. High occurrence of Shiga toxin-producing Escherichia coli (STEC) in healthy cattle in Rio de Janeiro State, Brazil. Vet. Microbiol., v.70, p.111-121, 1999.
5. Doyle, M.P.; Zhao, T.; Meng, J. et al. Escherichia coli O157:H7. Foodborne pathogenic bacteria. In: Food Microbiology Fundamentals and Frontiers. Washington, D.C.: Academic, 1997. p.171-191.
6. Gonzalez, A.G.M.; Coutinho, C.A.S.; Cerqueira, A.M.F. et al. Virulence markers of shiga toxin-producing Escherichia coli (STEC) O157:H7 and non-O157 isolated from healthy cattle in Rio de Janeiro State, Brazil. In: Anais do Congresso Brasileiro de Microbiologia, 21., 2001, Foz do Iguaçu. Anais... Foz do Iguaçu: SBM, 2001. p.104. (Resumo).
7. Foodborne diseases, emerging. World Health Organization, Janeiro de 2002. Disponível em: Acessado em: 30 out. 2004.
8. Griffin, P.M.; Tauxe, R.V. The epidemiology of infections caused by Escherichia coli O157:H7, other enterohemorrhagic E. coli and the associated hemolytic uremic syndrome. Epidemiol. Rev., v.13, p.60-98, 1991.
9. Hu, Y.; Zhang, Q.; Meitzler, J.C. Rapid and sensitive detection of Escherichia coli O157:H7 in bovine faeces by a multiplex PCR. J. Appl. Microbiol., v.87, p.867-876, 1999.
10. Jersek, B.; Tcherneva, E.; Rijpens, N. et al. Repetitive element sequence-based PCR for species and strain discrimination in the genus Listeria. Lett. Appl. Microbiol., v.23, p.55-60, 1996.
11. Kuntz, T.B.;Kuntz, S.T. Enterohemorrhagic E. coli infection. Primary Care Update Ob/Gyns, v.6, p.192-196, 1999.
12. Li Y.; Mustapha, A. Simultaneous detection of Escherichia coli O157:H7, Salmonella and Shigella in apple cider and produce by a multiplex PCR. J. Food Protec., v.67, p.27-33, 2004.
13. Macêdo, N.R.; Menezes, C.P.L.; Lage, A.P. et al. Detecção de cepas patogênicas pela PCR multiplex e avaliação da sensibilidade a antimicrobianos de Escherichia coli isoladas de leitões diarréicos. Arq. Bras. Med. Vet. Zootec., v.59, p.1117-1123, 2007.
14. Marek, P.; Nair, M.K.M.; Hoagland, T. et al. Survival and growth characteristics of Escherichia coli O157:H7 in pasteurized and unpasteurized Cheddar cheese whey. Int. J. Food Microbiol., v.94, p.1-7, 2004.
15. Mechie, S.C.; Chapman, P.A.; Siddons, C.A. A fifteen month study of Escherichia coli O157:H7 in a dairy herd. Epidemiol. Infect., v.188, p.17-25, 1997.
16. Mesquita, A.J.; Durr, J.W.; Coelho, K.O. Perspectivas e avanços da qualidade do leite no Brasil. Goiânia: Talento, 2006. 352p.
17. Nunes, E.L.C.; Santos, K.R.N.; Mondino, P.J.J. et al. Detection of ileS-2 gene encoding mupirocin resistance in methicillin-resistant Staphylococcus aureus by multiplex PCR. Diag. Microbiol. Infect. Dis., v.34, p.77-81, 1999.
18. Silveira, N.F.A.; Silva, N.; Contreras, C. et al. Occurrence of Escherichia coli O157:H7 in hamburgers produced in Brazil. J. Food Protec., v.62, p.1333-1335, 1999.