Detection of pathogenic strains by multiplex PCR and antimicrobial sensitivity of Escherichia coli isolated from piglets;Detecção de cepas patogênicas pela PCR multiplex e avaliação da sensibilidade a antimicrobianos de Escherichia coli isoladas de leitões diarréicos

Arquivo Brasileiro de Medicina Veterinaria e Zootecnia

Volumn 59, Issue 5, 2007, Pages 1117-1123

  Macedo N R ^a,e   Menezes, C P L ^b   Lage, A P ^a,f   Ristow, L E ^c   Reis, A ^d   Guedes, R M C ^a  

^a FEDERAL UNIVERSITY OF MINAS GERAIS   (Brazil)

^b Laboratório Biocod   (Brazil)

^c Laboratório TECSA   (Brazil)

^d IPEVE   (Brazil)

^e FAPEMIG Pfizer

^f CNPq ^*

Author keywords

Antimicrobial sensitivity; Escherichia coli; Multiplex PCR; Pig; Typification

Indexed keywords

ESCHERICHIA COLI; SUIDAE;

EID: 38149072926     PISSN: 01020935     EISSN: 16784162     Source Type: Journal    
DOI: 10.1590/s0102-09352007000500005     Document Type: Article

References (25)

1. BACCARO, M.R.; MORENO, A.M.; BRONZONI, P.V.M., et al. Detecção de genes codificadores de enterotoxinas e verotoxinas através da PCR em amostras de E. coli isoladas de leitões lactentes. In: CONGRESSO BRASILEIRO DE VETERINÁRIOS ESPECIALISTAS EM SUINOS, 9., 1999, Concórdia. Anais...Concórdia: EMBRAPA: CNPSA, 1999.
2. BACCARO, M.R.; MORENO, A.M.; FERREIRA, A.J.P. et al. Occurrence of some virulence genes among Escherichia coli isolates piglets in Brazil. In: INTERNATIONAL PIG VETERINARY SOCIETY CONGRESS, 16., 2000, Melbourne. Anais...Melbourne: International Pig Veterinary Society, 2000. p.52.
3. BACCARO, M.R.; MORENO, A.M.; CORRÊA, A. et al. Resistência antimicrobiana de amostras de Escherichia coli isoladas de fezes de leitões com diarréia. Arq. Inst. Biol., v.69, p.15-18, 2002.
4. BOROWSKI, S.M.; BARCELLOS, D.E.S.N.; STEPAN, A.L. et al. Sensibilidade a antimicrobianos de amostras de Escherichia coli isoladas de suínos apresentando diarréia no período pós-desmame. Arq. Fac. Vet. UFRGS, v.22, p.24-30, 1994.
5. BRITO, B.G.; TAGLIARI, K.C. Sensibilidade antimicrobiana de amostras de Escherichia coli isoladas de leitões lactentes com diarréia. Rev. Bras. Cien. Vet., v.7, p.117-119, 2000.
6. CALDERARO, F.F.; BACCARO, M.R.; MORENO, A.M. et al. Freqüência de agentes causadores de enterites em leitões lactentes provenientes de sistemas de produção de suínos de Estado de São Paulo. Arq. Inst. Biol., v.68, p.29-34, 2001.
7. CASEY, T.A.; NAGY, B.; MOON, H.W. Pathogenicity of porcine enterotoxigenic Escherichia coli that do not express K88, K99, F41 or 987P adhesions. Am. J. Vet. Res., v.53, p.1488-1492, 1992.
8. CHO, W.S.; PARK, Y.C.; CHAE, C. Genotypic prevalence for fimbriae and enterotoxins in Escherichia coli isolated from diarrheic piglets in Korea. In: INTERNATIONAL PIG VETERINARY SOCIETY CONGRESS, 16., 2000, Melbourne. Anais... Melbourne: International Pig Veterinary Society, 2000. p.54. (Resumo).
9. COSTA, M.M.; SILVA, M.A.; SPRICIGO, D.A. et al. Caracterizaç ão epidemiológica e perfil de resistência aos antimicrobianos de Escherichia coli isoladas de criatórios suínos do sul do Brasil. Pesq. Vet. Bras., v.26, p.5-8, 2006.
10. FAIRBROTHER, J.M.; HIGGINS, R.; DESAUTELS, C. Trends in path types and antimicrobial resistance of Escherichia coli isolated from pigs with postweaning diarrhea in Quebec. In: INTERNATIONAL PIG VETERINARY SOCIETY CONGRESS, 16., 2000, Melbourne. Anais...Melbourne: International Pig Veterinary Society, 2000. p.17. (Resumo).
11. FAIRBROTHER, J.M.; GYLES, C.L. Escherichia coli infections. In: STRAW, B.E.; ZIMMERMAN, J.J.; D'ALLAIRE, S. et al. (Ed). Disease of Swine. Oxford: Blacwell Science, 2006. p. 639-674.
12. FRANCIS, D.H.; GRANGE, P.A.; ZEMAN, D.H. et al. Expression of mucin-type glycoprotein K88 receptors strongly correlates with piglet susceptibility to K88+ enterotoxigenic Escherichia coli, but adhesion of this bacterium to brush borders does not. Infect. Immun., v.66, p.4050-4055, 1998.
13. FRANCIS, D.H. Enterotoxigenic Escherichia coli infection in pigs and its diagnosis. J. Swine Health Prod., v.10, p.171-175, 2002.
14. HARIHARAN, H.; COLES, M.; POOLE, D. et al. Antibiotic resistance among enterotoxigenic Escherichia coli from piglets and calves with diarrhea. Can. Vet. J., v.45, p.605-606, 2004.
15. HITCHINS, A.D.; FENG, P.; WATKINS, W.D. et al. Escherichia coli and the coliform bacteria. In: Bacterological Analytical Manual. Gaithersbrug: AOAC International, 1995. p.4.01-4.29.
16. HOLAND, R.E. Some infectious causes of diarrhea in young farm animals. Clin. Microbiol. Rev., v.3, p.345-375, 1990.
17. IMBERECHTS, H.; BERTSCHINGER, H.U.; NAGY, B. et al. Fimbrial colonization factors F18ab and F18ac of Escherichia coli isolated from pigs with postweaning diarrhea and edema disease. Adv. Exper. Med. Biol., v.412, p.175-183, 1997.
18. MOON, H.W.; SCHINEIDER, R.A.; MOSELY, S.L. Comparative prevalence of four enterotoxin genes among Escherichia coli isolates from swine. Am. J. Vet. Res., v.47, p.210-212, 1986.
19. MULLANEY, C.D.; FRANCIS, K.H.; WILLGOHS, J.A. Comparison of seroagglutination, ELISA, and indirect fluorescent antibody staining for the detection of K99, K88, and 987P pilus antigens of Escherichia coli. J. Vet. Diagn. Invest., v.3, p.115-118, 1991.
20. NATARO, J.P.; KAPER, J.B. Diarrheagenic Escherichia coli. Clin. Microbiol. Rev., v.11, p. 142-201, 1998.
21. SAMBROOK, J.; FRITSCH, E.F.; MANIATIS, T. (Ed). Molecular cloning: A Laboratory Manual. New York: Cold Spring Harbor, 1989. p.E.3-E.4.
22. VARGAS, A. C.; SILVA, M.S.; COSTA, M.M. et al. Perfil epidemiológico, molecular e de resistência aos antimicrobianos de amostras de Escherichia coli isoladas em criatórios suínos. In: CONGRESSO BRASILEIRO DE VETERINÁRIOS ESPECIALISTAS EM SUÍNOS, 11., 2003, Goiânia. Anais...Goiânia: ABRAVES, 2003. p.03-04. (Resumo).
23. WAJJAWALKU, W.; SIRIPATTARAPRAVAT, K.; BILHMAD, U. et al. Frequence of virulent factors in hemolytic Escherichia coli isolated from pigs by using polymerase chain reaction. In: INTERNATIONAL PIG VETERINARY SOCIETY CONGRESS, 17., 2002, Ames. Anais... Ames: International Pig Veterinary Society, 2002. p.57. (Resumo).
24. WILSON, R.A.; FRANCIS, D.H. Fimbriae and enterotoxins associated with E. coli serogroups isolated from clinical cases of porcine colibacillosis. Am. J. Vet. Res., v.47, p.213-217, 1986.
25. WOODS, G.L.; WASHINGTON, J.A. Antibacterial susceptibility tests: dilution and disk diffusion methods. In: MURRAY, P.R.; BARON, E.J.; PFALLER, M.A. et al. Manual of clinical microbiology. 6.ed. Washington: ASM Press, 1995. p.1327-1341.