Positive fluorescent selection permits precise, rapid, and in-depth overexpression analysis in plant protoplasts

Plant Physiology

Volumn 149, Issue 3, 2009, Pages 1231-1239

  Bargmann, Bastiaan O R ^a   Birnbaum, Kenneth D ^a  

^a NEW YORK UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

METAZOA;

EID: 63549106001     PISSN: 00320889     EISSN: 15322548     Source Type: Journal    
DOI: 10.1104/pp.108.133975     Document Type: Article

References (26)

1. Birnbaum K, Shasha DE, Wang JY, Jung JW, Lambert GM, Galbraith DW, Benfey PN (2003) A gene expression map of the Arabidopsis root. Science 302: 1956-1960
2. Brady SM, Orlando DA, Lee JY, Wang JY, Koch J, Dinneny JR, Mace D, Ohler U, Benfey PN (2007) A high-resolution root spatiotemporal map reveals dominant expression patterns. Science 318: 801-806
3. Cummins I, Steel PG, Edwards R (2007) Identification of a carboxylesterase expressed in protoplasts using fluorescence-activated cell sorting. Plant Biotechnol J 5: 354-359
4. De Sutter V, Vanderhaeghen R, Tilleman S, Lammertyn F, Vanhoutte I, Karimi M, Inze' D, Goossens A, Hilson P (2005) Exploration of jasmonate signalling via automated and standardized transient expression assays in tobacco cells. Plant J 44: 1065-1076
5. Dinneny JR, Long TA, Wang JY, Jung JW, Mace D, Pointer S, Barron C, Brady SM, Schiefelbein J, Benfey PN (2008) Cell identity mediates the response of Arabidopsis roots to abiotic stress. Science 320: 942-945
6. Galbraith DW, Lambert GM, Grebenok RJ, Sheen J (1995) Flow cytometric analysis of transgene expression in higher plants: green-fluorescent protein. Methods Cell Biol 50: 3-14
7. Gifford ML, Dean A, Gutierrez RA, Coruzzi GM, Birnbaum KD (2008) Cell-specific nitrogen responses mediate developmental plasticity. Proc Natl Acad Sci USA 105: 803-808
8. Guilfoyle TJ, Hagen G (2007) Auxin response factors. Curr Opin Plant Biol 10: 453-460
9. Harkins KR, Galbraith DW (1984) Flow sorting and culture of plant protoplasts. Physiol Plant 60: 43-52
10. Haseloff J, Siemering KR (2006) The uses of green fluorescent protein in plants. Methods Biochem Anal 47: 259-284
11. Haugland RP (2002) Handbook of Fluorescent Probes and Research Products. Molecular Probes, Eugene, OR
12. Lee JY, Colinas J, Wang JY, Mace D, Ohler U, Benfey PN (2006) Transcriptional and posttranscriptional regulation of transcription factor expression in Arabidopsis roots. Proc Natl Acad Sci USA 103: 6055-6060
13. Lee MM, Schiefelbein J (1999) WEREWOLF, a MYB-related protein in Arabidopsis, is a position-dependent regulator of epidermal cell patterning. Cell 99: 473-483
14. Lin J, Wang Y, Wang G (2006) Salt stress-induced programmed cell death in tobacco protoplasts is mediated by reactive oxygen species and mitochondrial permeability transition pore status. J Plant Physiol 163: 731-739
15. Looger LL, Lalonde S, Frommer WB (2005) Genetically encoded FRET sensors for visualizing metabolites with subcellular resolution in living cells. Plant Physiol 138: 555-557
16. Moore I, Samalova M, Kurup S (2006) Transactivated and chemically inducible gene expression in plants. Plant J 45: 651-683
17. Müller P, Kuttenkeuler D, Gesellchen V, Zeidler MP, Boutros M (2005) Identification of JAK/STAT signalling components by genome-wide RNA interference. Nature 436: 871-875
18. Ottenschläger I, Wolff P, Wolverton C, Bhalerao RP, Sandberg G, Ishikawa H, Evans M, Palme K (2003) Gravity-regulated differential auxin transport from columella to lateral root cap cells. Proc Natl Acad Sci USA 100: 2987-2991
19. Palmer EL, Miller AD, Freeman TC (2006) Identification and characterisation of human apoptosis inducing proteins using cell-based transfection microarrays and expression analysis. BMC Genomics 7: 145
20. Sheen J (2001) Signal transduction in maize and Arabidopsis mesophyll protoplasts. Plant Physiol 127: 1466-1475
21. Sheen J, Hwang S, Niwa Y, Kobayashi H, Galbraith DW (1995) Greenfluorescent protein as a new vital marker in plant cells. Plant J 8: 777-784
22. Tiwari S, Wang S, Hagen G, Guilfoyle TJ (2006) Transfection assays with protoplasts containing integrated reporter genes. Methods Mol Biol 323: 237-244
23. Tiwari SB, Wang XJ, Hagen G, Guilfoyle TJ (2001) AUX/IAA proteins are active repressors, and their stability and activity are modulated by auxin. Plant Cell 12: 2809-2822
24. Ulmasov T, Murfett J, Hagen G, Guilfoyle TJ (1997) Aux/IAA proteins repress expression of reporter genes containing natural and highly active synthetic auxin response elements. Plant Cell 9: 1963-1971
25. Weijers D, Jürgens G (2004) Funneling auxin action: specificity in signal transduction. Curr Opin Plant Biol 7: 687-693
26. Yoo SD, Cho YH, Sheen J (2007) Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis. Nat Protocols 2: 1565-1572