A review of the current state of biodiesel production using enzymatic transesterification

Biotechnology and Bioengineering

Volumn 102, Issue 5, 2009, Pages 1298-1315

  Fjerbaek, Lene ^a   Christensen, Knud V ^a   Norddahl, Birgir ^a  

^a UNIVERSITY OF SOUTHERN DENMARK   (Denmark)

Author keywords

Biodiesel; Enzyme; Lipase; Productivity; Transesterification

Indexed keywords

BIODIESEL PRODUCTIONS; CHEMICAL CATALYSTS; CRITICAL ANALYSIS; CURRENT STATUS; ENZYMATIC CATALYSIS; ENZYMATIC TRANSESTERIFICATION; INDUSTRIAL PRODUCTIONS; MASS TRANSFER LIMITATIONS; REACTION TIME; REACTOR CONFIGURATIONS; WATER ACTIVITIES;

BIODIESEL; CATALYSIS; CATALYSTS; ESTERIFICATION; PILOT PLANTS; TRANSESTERIFICATION;

ENZYMES;

2 BUTANOL; 2 ETHYLHEXANOL; ACETIC ACID ETHYL ESTER; ALCOHOL; BIODIESEL; BUTANOL; FAT; ISOBUTANOL; ISOPENTYL ALCOHOL; METHANOL; METHYL ACETATE; OIL; PROPANOL; RAPESEED OIL; SOLVENT; TRIACYLGLYCEROL LIPASE; UNCLASSIFIED DRUG;

CATALYSIS; CHEMICAL REACTION; EVALUATION RESEARCH; IMMOBILIZATION; OIL INDUSTRY; PARTICLE SIZE; REACTOR; REVIEW; TEMPERATURE SENSITIVITY; TRANSESTERIFICATION;

BIOTECHNOLOGY; BIOTRANSFORMATION; CHINA; ENZYMES; GASOLINE; RESEARCH;

EID: 63549102137     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22256     Document Type: Review

References (115)

1. Abigor RD, Uadia PO, Foglia TA, Haas MJ, Jones KC, Okpefa E, Obibuzor JU, Bafor ME. 2000. Lipase-catalyzed production of biodiesel fuel from some Nigerian lauric oils. Biochem Soc Trans 28(6):979-981.
2. Al-Zuhair S. 2005. Production of biodiesel by lipase-catalyzed transester- ification of vegetable oils: A kinetics study. Biotechnol Prog 21(5): 1442-1448.
3. Aris R. 2000. Elementary chemical reactor analysis. New York: Dover Publications.
4. Balcão VM, Paiva AL, Malcata FX. 1996. Bioreactors with immobilized lipases: State of the art. Enzyme Microb Technol 18(6):392-416.
5. Ban K, Hama S, Nishizuka K, Kaieda M, Matsumoto T, Kondo A, Noda H, Fukuda H. 2002. Repeated use of whole-cell biocatalysts immobilized within biomass support particles for biodiesel fuel production. J Mol Catal B: Enzym 17(3-5):157-165.
6. Belafibako K, Kovács F, Gubicza L, Hancsók J. 2002. Enzymatic biodiesel production from sunflower oil by Candida antarctica lipase in a solvent-free system. Biocatal Biotransform 20(6):437-439.
7. Biselli M, Kragl U, Wandrey C. 2002. Reaction engineering for enzyme- catalyzed biotransformations. In: Drauz K, Waldmann H, editors. Enzyme catalysis in organic synthesis. Weinheim, Germany: Wiley-VCH. p 185-257.
8. Bourdart M, Djega-Mariadassou G. 1984. Kinetics of heterogenous catalytic reactions. New Jersey: Princeton University Press.
9. Cao P, Tremblay AY, Dubé MA, Morse K. 2007. Effect of membrane pore size on the performance of a membrane reactor for biodiesel production. Ind Eng Chem Res 46(l):52-58.
10. Chen JW, Wu WT. 2003. Regeneration of immobilized Candida antarctica lipase for transesterification. J Biosci Bioeng 95(5):466-469.
11. Darnoko D, Cheryan M. 2000. Continuous production of palm methyl esters. J Am Oil Chem Soc 77(12):1269-1272.
12. Demirbas A. 2007. Progress and recent trends in biofuels. Prog Energy Combust Sci 33(1):1-18.
13. Deng L, Tan T, Fang W, Xu X. 2003. Enzymatic production of fatty acid alkyl esters with a lipase preparation from Candida sp. 99-125. Eur J Lipid Sci Technol. 105(12):727-734.
14. Deng L, Xu XB, Haraldsson GG, Tan TW, Wang F. 2005. Enzymatic production of alkyl esters through alcoholysis: A critical evaluation of lipases and alcohols. J Am Oil Chem Soc 82(5):341-347.
15. Dossat V, Combes D, Marty A. 1999. Continuous enzymatic transesteri- fication of high oleic sunflower oil in a packed bed reactor: Influence of the glycerol production. Enzyme Microb Technol 25(3-5):194-200.
16. Dossat V, Combes D, Marty A. 2002. Lipase-catalysed transesterification of high oleic sunflower oil. Enzyme Microb Technol 30(l):90-94.
17. Drown DC, Harper K, Frame EA. 2001. Screening vegetable oil alcohol esters as fuel lubricity enhancers. J Am Oil Chem Soc 78(6):579-584.
18. Du W, Xu YY, Liu DH, Zeng J. 2004. Comparative study on lipase-catalyzed transformation of soybean oil for biodiesel production with different acyl acceptors. J Mol Catal B: Enzym 30(3-4):125-129.
19. Du W, Wang L, Liu D. 2007. Improved methanol tolerance during Novozym435-mediated methanolysis of SODD for biodiesel produc- tion. Green Chem 9(2): 173-176.
20. Du W, Li W, Sun T, Chen X, Liu D. 2008. Perspectives for biotechnological production of biodiesel and impacts. Appl Microbiol Biotechnol 79(3):331-337.
21. Dubé MA, Tremblay AY, Liu J. 2007. Biodiesel production using a mem- brane reactor. Bioresour Technol 98(3):639-647.
22. FAO. 2008. Food and Agriculture Organization of the United Nations. Food Outlook-Global Market Analysis. Food Outlook (GIEWS). June 2008.
23. Freedman B, Pryde EH, Mounts TL. 1984. Variables affecting the yields of fatty esters from transesterified vegetable oils. J Am Oil Chem Soc 61(10):1638-1643.
24. Froment GF, Bischoff KB. 1990. Chemical reactor analysis and design. New York: John Wiley & Sons, Inc.
25. Fukuda H, et al. 2001. Biodiesel fuel production by transesterification of oils. J Biosci Bioeng 92(5):405-416.
26. Garcia HS, Malcata FX, Hill CG, Amundson CH. 1992. Use of Candida rugosa lipase immobilized in a spiral wound membrane reactor for the hydrolysis of milkfat. Enzyme Microb Technol 14(7):535-545.
27. Haas MJ, McAloon AJ, Yee WC, Foglia TA. 2006. A process model to estimate biodiesel production costs. Bioresour Technol 97:671-678.
28. Hari Krishna S, Karanth NG. 2001. Lipase-catalyzed synthesis of isoamyl butyrate: A kinetic study. Biochim Biophys Acta 1547(2):262-267.
29. Hernández-Martín E, Otero C. 2008. Different enzyme requirements for the synthesis of biodiesel: Novozym* 435 and Lipozyme* TL IM. Bior- esour Technol 99(2):277-286.
30. Hilal N, Nigmatullin R, Alpatova A. 2004. Immobilization of cross-linked lipase aggregates within mίcroporous polymeric membranes. J Membr Sci 238(1-2):131-141.
31. Hoq MM, Yamane T, Shimizu S. 1985. Continuous hydrolysis of olive oil by lipase in microporous hydrophobic membrane bioreactor. J Am Oil Chem Soc 62(6):1016-1021.
32. Hsu AF, Jones K, Marnier WN, Foglia TA. 2001. Production of alkyl esters from tallow and grease using lipase immobilized in a phyllosilicate sol- gel. J Am Oil Chem Soc 78(6): 585-588.
33. Hsu AF, Jones KC, Foglia TA, Marmer WN. 2004. Continuous production of ethyl esters of grease using an immobilized lipase. J Am Oil Chem Soc 81(8):749-752.
34. IEA. 2008. International Energy Agency. IEA Statistics. Oil and Renewables. http://www.iea.org.
35. Iso M, Chen B, Eguchi M, Kudo T, Shrestha S. 2001. Production of biodiesel fuel from triglycerides and alcohol using immobilized lipase. J Mol Catal B: Enzym 16(l):53-58.
36. Jaeger KE, Eggert T. 2002. Lipases for biotechnology. Curr Opin Biotechnol 13(4):390-397.
37. Kaieda M, Samukawa T, Matsumoto T, Ban K, Kondo A, Shimada Y, Noda H, Nomoto F, Ohtsuka K, Izumoto E, Fukuda H. 1999. Biodiesel fuel production from plant oil catalyzed by Rhizopus oryzae lipase in a water-containing system without an organic solvent. J Biosci Bioeng 88(6):627-631.
38. Kaieda M, Samukawa T, Kondo A, Fukuda H. 2001. Effect of methanol and water contents on production of biodiesel fuel from plant oil catalyzed by various lipases in a solvent-free system. J Biosci Bioeng 91(1):12-15.
39. Keskin A, Gürü M, Altiparmak D, Aydin K. 2008. Using of cotton oil soapstock biodiesel-diesel fuel blends as an alternative diesel fuel. Renew Energ 33(4):553-557.
40. Knezevic Z, Mojovic L, Adnadjevic B. 1998. Palm oil hydrolysis by lipase from Candida cylindracea immobilized on zeolite type Y. Enzyme Microb Technol 22(4):275-280.
41. Kojima S, Du D, Sato M, Park EY. 2004. Efficient production of fatty acid methyl ester from waste activated bleaching earth using diesel oil as organic solvent. J Biosci Bioeng 98(6):420-424. Romers K, Stloukal R, Machek J, Skopal F. 2001. Biodiesel from rapeseed oil, methanol and KOH 3: Analysis of composition of actual reaction mixture. Eur J Lipid Sci Technol 103(6):363-371.
42. Köse O, Tüter M, Aksoy HA. 2002. Immobilized Candida antarctka lipase- catalyzed alcoholysis of cotton seed oil in a solvent-free medium. Bioresour Technol 83(2):125-129.
43. Krawczyk T. 1996. Biodiesel: Alternative fuel makes inroads but hurdles remain. Inform 7(8):801-815.
44. Kulkarni MG, Dalai AK. 2006. Waste cooking oil-An economical source for biodiesel: A review. Ind Eng Chem Res 45(9):2901-2913.
45. Kumari V, Shah S, Gupta MN. 2007. Preparation of biodiesel by lipase- catalyzed transesterification of high free fatty acid containing oil from Madhuca indica. Energy Fuels 21(l):368-372.
46. Lai CC, Zullaikah S, Vali SR, Ju YH. 2005. Lipase-catalyzed production of biodiesel from rice bran oil. J Chem Technol Biotechnol 80(3):331-337.
47. Lee I, Johnson LA, Hammond EG. 1995. Use of branched-chain esters to reduce the crystallization temperature of biodiesel. J Am Oil Chem Soc 72(10):1155-1160.
48. Lee K-T, Foglia TA, Chang KS. 2002. Production of alkyl ester as biodiesel from fractionated lard and restaurant grease. J Am Oil Chem Soc 79(2): 191-195.
49. Lee DH, Kim JM, Shin HY, Kang SW, Kim SW. 2006. Biodiesel production using a mixture of immobilized Rhizopus oryzae and Candida rugosa lipases. Biotechnol Bioprocess Eng 11(6)522-525.
50. Li L, Du W, Liu D, Wang L, Li Z. 2006. Lipase-catalyzed transesterification of rapeseed oils for biodiesel production with a novel organic solvent as the reaction medium. J Mol Catal B: Enzym 43(1-4):58-62.
51. Linko YY, Lamsa M, Huhtala A, Rantanen 0.1995. Lipase biocatalysis in the production of esters. J Am Oil Chem Soc 72(11):1293-1299.
52. Linko YY", Lamsa M, Wu X, Uosukainen E, Seppala J, Linko P. 1998. Biodegradable products by lipase biocatalysis. J Biotechnol 66(1):41-50.
53. Lu J, Nie K, Wang F, Tan T. 2008. Immobilized lipase Candida sp 99-125 catalyzed methanolysis of glycerol trioleate: Solvent effect. Bioresour Technol 99(14):6070-6074.
54. Ma F, Hanna MA. 1999. Biodiesel production: A review. Bioresour Technol 70(1):1-15.
55. Matsumoto T, Takahashi S, Kaieda M, Ueda M, Tanaka A, Fukuda H, Kondo A. 2001. Yeast whole-cell biocatalyst constructed by intracellular overproduction of Rhizopus oryzae lipase is applicable to biodiesel fuel production. Appl Microbiol Biotechnol 57(4):515-520.
56. Mears DE. 1971a. Diagnostic criteria for heat transport limitations in fixed bed reactors. J Catal 20:127-131.
57. Mears DE. 1971b. Tests for transport limitations in experimental catalytic reactors. Ind Eng Chem Process Des Dev 10(4):541-547.
58. Meher LC, Sagar DV, Naik SN. 2006. Technical aspects of biodiesel production by transesterification: A review. Renew Sustain Energ Rev 10(3):248-268.
59. Merçon F, Sant Anna GL, Jr., Nobrega R. 2000. Enzyme hydrolysis of babassu oil in a membrane bioreactor. J Am Oil Chem Soc 77(10): 1043-1048.
60. Miller C, Austin H, Posorske L, Gonzlez J. 1988. Characteristics of an immobilized lipase for the commercial synthesis of esters. J Am Oil Chem Soc 65(6):927-931.
61. Mittelbach M. 1990. Lipase catalyzed alcoholysis of sunflower oil. J Am Oil Chem Soc 67(3):168-170.
62. Mittelbach M, Worgetter M, Pernkopf J, Junek H. 1983. Diesel fuel derived from vegetable oils: Preparation and use of rape oil methyl-ester. Energ Agr 2(4):369-384.
63. Modi MK, Reddy JRC, Rao BVSK, Prasad RBN. 2007. Lipase-mediated conversion of vegetable oils into biodiesel using ethyl acetate as acyl acceptor. Bioresour Technol 98(6):1260-1264.
64. Montero S, Blanco A, Virto MD, Carlos LL, Agud I, Solozabal R, Lascaray JM, de Renobales M, Llama MJ, Serra JL. 1993. Immobilization of Candida rugosa lipase and some properties of the immobilized enzyme. Enzyme Microb Technol 15(3):239-247.
65. Nelson L, Foglia T, Marmer W. 1996. Lipase-catalyzed production of biodiesel. J Am Oil Chem Soc 73(9):1191-1195.
66. Nie K, Xie F, Wang F, Tan T. 2006. Lipase catalyzed methanolysis to produce biodiesel: Optimization of the biodiesel production. J Mol Catal B: Enzym 43(1-4):142-147.
67. Noureddini H, Harkey D, Medikonduru V. 1998. A continuous process for the conversion of vegetable oils into methyl esters of fatty acids. J Am Oil Chem Soc 75(12):1775-1783.
68. Noureddini H, Gao X, Philkana RS. 2005. Immobilized Pseudomonas cepacia lipase for biodiesel fuel production from soybean oil. Bioresour Technol 96(7):769-777.
69. Orcaire O, Buisson P, Pierre AC. 2006. Application of silica aerogel encapsulated lipases in the synthesis of biodiesel by transesterification reactions. J Mol Catal B: Enzym 42(3-4):106-113.
70. Paiva AL, Balcao VM, Malcata FX. 2000. Kinetics and mechanisms of reactions catalyzed by immobilized lipases. Enzyme Microb Technol 27(3-5): 187-204.
71. Park EY, Sato M, Kojima S. 2006. Fatty acid methyl ester production using lipase-immobilizing silica particles with different sizes and different specific surface areas. Enzyme Microb Technol 39:889-896.
72. Park EY, Sato M, Kojima S. 2008. Lipase-catalyzed biodiesel production from waste activated bleaching earth as raw material in a pilot plant. Bioresour Technol 99:3130-3135.
73. Paula AV, Urioste D, Santos JC, de Castro HF. 2007. Porcine pancreatic lipase immobilized on polysiloxane-polyvinyl alcohol hybrid matrix: Catalytic properties and feasibility to mediate synthesis of surfactants and biodiesel. J Chem Technol Biotechnol 82(3):281-288.
74. Poling BE, Pausnitz JM, O'Connell J. 2002. The properties of gases and liquids, 5th edn. USA: McGraw-Hill.
75. Posorske LH, LeFebvre GK, Miller CA, Hansen TT, Glenvig BL. 1988. Process considerations of continuous fat modification with an immobilized lipase. J Am Oil Chem Soc 65(6):922-926.
76. Pryde EH. 1983. Vegetable oils as diesel fuels: Overview. J Am Oil Chem Soc 60(8):1557-1558.
77. Royon D, Daz M, Ellenrieder G, Locatelli S. 2007. Enzymatic production of biodiesel from cotton seed oil using t-butanol as a solvent. Bioresour Technol 98(3):648-653.
78. Salis A, Pinna M, Monduzzi M, Solinas V. 2005. Biodiesel production from triolein and short chain alcohols through biocatalysis. J Biotechnol 119(3):291-299.
79. Samukawa T, Kaieda M, Matsumoto T, Ban K, Kondo A, Shimada Y, Noda H, Fukuda H. 2000. Pretreatment of immobilized Candida antarctka lipase for biodiesel fuel production from plant oil. J Biosci Bioeng 90(2):180-183.
80. Shah S, Gupta MN. 2007. Lipase catalyzed preparation of biodiesel from ¡atropha oil in a solvent-free system. Process Biochem 42(3):409-414.
81. Shaw JF, Chang SW, Lin SC, Wu TT, Ju HY, Akoh CC, Chang RH, Shieh CJ. 2008. Continuous enzymatic synthesis of biodiesel with Novozym 435. Energy Fuels 22(2):840-844.
82. Sheehan J, Camobreco V, Duffield J, Graboski M, Shapouri H. 1998. An overview of biodiesel and petroleum diesel life cycles. NREL/ TP-580-24772: 1-60. National Renewable Energy Laboratory (NREL) and US-Department of Energy (DOE). Report.
83. Sheldon RA, Schoevaart R, Van Langen LM. 2005. Cross-linked enzyme aggregates (CLEAs): A novel and versatile method for enzyme immobilization (a review). Biocatal Biotransform 23(3-4):141-147.
84. Shibasaki-Kitakawa N, Honda H, Kuribayashi H, Toda T, Fukumura T, Yonemoto T. 2007. Biodiesel production using anionic ion-exchange resin as heterogeneous catalyst. Bioresour Technol 98:416-421.
85. Shimada Y, Watanabe Y, Samukawa T, Sugihara A, Noda H, Fukuda H, Tominaga Y. 1999. Conversion of vegetable oil to biodiesel using immobilized Candida antarctka lipase. J Am Oil Chem Soc 76(7): 789-793.
86. Shimada Y, Watanabe Y, Sugihara A, Tominaga Y. 2002. Enzymatic alcoholysis for biodiesel fuel production and application of the reaction to oil processing. J Mol Catal B: Enzym 17(3-5): 133-142.
87. Soumanou MM, Bornscheuer UT. 2003. Improvement in lipase-catalyzed synthesis of fatty acid methyl esters from sunflower oil. Enzyme Microb Technol 33(1):97-103.
88. Srivastava A, Prasad R. 2000. Triglycerides-based diesel fuels. Renew Sustain Energ Rev 4(2):111-133.
89. Stevenson DE, Stanley RA, Furneaux RH. 1994. Near-quantitative produc- tion of fatty acid alkyl esters by lipase-catalyzed alcoholysis of fats and oils with adsorption of glycerol by silica gel. Enzyme Microb Technol 16(6):478-484.
90. Suehara K, Kawamoto Y, Fujii E, Kohda J, Nakano Y, Yano T. 2005. Biological treatment of wastewater discharged from biodiesel fuel production plant with alkali-catalyzed transesterification. J Biosci Bioeng 100(4):437-442.
91. Suppes GJ, Bockwinkel K, Lucas S, Botts JB, Mason MH, Heppert JA. 2001. Calcium carbonate catalyzed alcoholysis of fats and oils. J Am Oil Chem Soc 78(2):139-145.
92. Suppes GJ, Dasari MA, Doskocil EJ, Mankidy PJ, Goff MJ. 2004. Transes- terification of soybean oil with zeolite and metal catalysts. Appl Catal A 257(2):213-223.
93. Tan TW, Nie KL, Wang F. 2006. Production of biodiesel by immobilized Candida sp. lipase at high water content. Appl Biochem Biotechnol 128(2):109-116.
94. Torres CF, Moeljadi M, Hill CG. 2003. Lipase-catalyzed ethanolysis of fish oils: Multi-response kinetics. Biotechnol Bioeng 83(3):274-281.
95. Torres CF, Tore AM, Fornari T, Senorans FJ, Reglero G. 2007. Ethanolysis of a waste material from olive oil distillation catalyzed by three different commercial lipases: A kinetic study. Biochem Eng J 34(2):165-171.
96. Trubiano G, Borio D, Errazu A. 2007. Influence of the operating conditions and the external mass transfer limitations on the synthesis of fatty acid esters using a Candida antarctka lipase. Enzyme Microb Technol 40(4): 716-722.
97. Venendaal R, Jorgensen U, Foster CA. 1997. European energy crops: A synthesis. Biomass Bioenergy 13(3):147-185.
98. Voet D, Voet JG. 2004. Biochemistry. New York: Wiley International Edition.
99. Wang PS, Tat ME, Van Gerpen J. 2005. The production of fatty acid isopropyl esters and their use as a diesel engine fuel. J Am Oil Chem Soc 82(ll):845-849.
100. Watanabe Y, Shimada Y, Sugihara A, Tominaga Y. 1999. Stepwise etha- nolysis of tuna oil using immobilized Candida antarctka lipase. J Biosci Bioeng 88(6):622-626.
101. Watanabe Y, Shimada Y, Sugihara A, Noda H, Fukuda H, Tominaga Y. 2000. Continuous production of biodiesel fuel from vegetable oil using immobilized Candida antarctka lipase. J Am Oil Chem Soc 77(4):355-360.
102. Watanabe Y, Shimada Y, Sugihara A, Tominaga Y. 2001. Enzymatic conversion of waste edible oil to biodiesel fuel in a fixed-bed bioreactor. J Am Oil Chem Soc 78(7):703-707.
103. Watanabe Y, Shimada Y, Sugihara A, Tominaga Y. 2002. Conversion of degummed soybean oil to biodiesel fuel with immobilized Candida antarctka lipase. J Mol Catal B: Enzym 17(3-5):151-155.
104. Watanabe Y, Pinsirodom P, Nagao T, Kobayashi T, Nishida Y, Takagi Y, Shimada Y. 2005. Production of FAME from acid oil model using immobilized Candida antarctka lipase. J Am Oil Chem Soc 82( 11 ):825-831.
105. Watanabe Y, Pinsirodom P, Nagao T, Yamauchi A, Kobayashi T, Nishida Y, Takagi Y, Shimada Y. 2006. Conversion of acid oil by-produced in vegetable oil refining to biodiesel fuel by immobilized Candida ant- arctka lipase. J Mol Catal B: Enzym 44:99-105.
106. Wei D, Xu YY, Jing Z, Liu DH. 2004. Novozym 435-catalysed transester- ification of crude soya bean oils for biodiesel production in a solvent- free medium. Biotechnol Appl Biochem 40:187-190.
107. Wilson L, Femandez-Lorente G, Fernandez-Laluente R, Illanes A, Guisan JM, Palomo JM. 2006. CLEAs of lipases and poly-ionic polymers: A simple way of preparing stable biocatalysts with improved properties. Enzyme Microb Technol 39(4):750-755.
108. Wu WH, Foglia TA, Marmer WN, Phillips JG. 1999. Optimizing produc- tion of ethyl esters of grease using 95% ethanol by response surface methodology. J Am Oil Chem Soc 76(4):517-521.
109. Xu X. 2000. Modification of oils and fats by lipase-catalyzed interesterifica- tion: Aspects of process engineering. In: Bornscheuer UT, editor. Enzymes in lipid modification. Weinheim, Germany: Wiley-VCH. p 190-215.
110. Xu Y, Du W, Liu D. 2005. Study on the kinetics of enzymatic inte- resterification of triglycerides for biodiesel production with methyl acetate as the acyl acceptor. J Mol Catal B: Enzym 32(5-6):241-245.
111. Yamane T. 1987. Enzyme technology for the lipids industry: An engineering overview. J Am Oil Chem Soc 64(12): 1657-1662.
112. Yong YP, Al-Duri B. 1996. Kinetic studies on immobilised lipase esterification of oleic acid and octanol. J Chem Technol Biotechnol 65(3):239-248.
113. Zhang TZ, Yang LR, Zhu ZQ, Wu JP. 2002. The kinetic study on lipase- catalyzed transesterification of alpha-cyano-3-phenoxybenzyl alcohol in organic media. J Mol Catal B: Enzym 18(4-6):315-323.
114. Zhang Y, Dube MA, Mclean DD, Kates M. 2003. Biodiesel production from waste cooking oil. 1. Process design and technological assessment. Bioresour Technol 89(1):1-16.
115. Zhang TZ, Yang LR, Zhu ZQ. 2005. Determination of internal diffusion limitation and its macroscopic kinetics of the trans- esterification of CPB alcohol catalyzed by immobilized lipase in organic media. Enzyme Microb Technol 36(2-3):203-209.