Miniaturized GPCR signaling studies in 1536-well format

Journal of Biomolecular Techniques

Volumn 19, Issue 4, 2008, Pages 267-274

  Shultz, S ^a   Worzella, T ^a   Gallagher, A ^b   Shieh, J ^b   Goueli, S ^a   Hsiao, K ^a   Vidugiriene, J ^a  

^a PROMEGA CORPORATION   (United States)

^b Labcyte Inc (Deerac Fluidics)   (United States)

Author keywords

Bioluminescence; GPCR; HTS; Instrumentation

Indexed keywords

CYCLIC AMP; G PROTEIN COUPLED RECEPTOR;

ARTICLE; BIOTECHNOLOGY; DRUG ANTAGONISM; DRUG DEVELOPMENT; DRUG EFFECT; DRUG POTENTIATION; DRUG SCREENING; ELECTRONICS; EVALUATION; HUMAN; IN VITRO STUDY; INSTRUMENTATION; LUMINESCENCE; METABOLISM; METHODOLOGY; SIGNAL TRANSDUCTION; VALIDATION STUDY;

BIOTECHNOLOGY; CYCLIC AMP; DRUG DISCOVERY; DRUG EVALUATION, PRECLINICAL; HUMANS; LUMINESCENT MEASUREMENTS; MINIATURIZATION; RECEPTORS, G-PROTEIN-COUPLED; SIGNAL TRANSDUCTION;

EID: 63449132704     PISSN: 15240215     EISSN: 19434731     Source Type: Journal    
DOI: None     Document Type: Article

References (6)

1. Ellis C. The state of GPCR research in 2004. Nat Rev Drug Discovery 2004;3:577-626.
2. Kumar M, Hsiao K, Vidugiriene, and Goueli SA. A Bioluminescent-Based, HTS-Compatible Assay to Monitor G-Protein-Coupled Receptor Modulation of Cellular Cyclic AMP. Assay Drug Dev Technol 2007;5(2): 237-245.
3. Ryman-Rasmussen JP, Nichols DE, and Mailman RB. Differential Activation of Adenylate Cyclase and Receptor Internalization by Novel Dopamine D1 Receptor Agonists. Mol Pharmacol 2005;68: 1039-1048.
4. Iorio LC, Barnett A, Leitz FH, Houser VP, and Korduba CA. SCH 23390, a potential benzazepine antipsychotic with unique interactions on dopaminergic systems. J Pharmacol Exp Ther 1983;226: 462-468.
5. Zhang JH, Chung TDY, and Oldenburg KR. A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays. J Biomolecular Screening 1999;4: 67-73.
6. 1280™ Compound List, Sigma-Aldrich, USA.