Highly efficient mRNA-based gene transfer in feeder-free cultured H9 human embryonic stem cells

Cloning and Stem Cells

Volumn 6, Issue 3, 2004, Pages 211-216

  Ponsaerts, Peter ^a   Van Der Sar, Sjaak ^b   Van Tendeloo, Viggo F I ^a   Jorens, Philippe G ^a   Berneman, Zwi N ^a   Singh, Prim B ^b  

^a ANTWERP UNIVERSITY HOSPITAL   (Belgium)

^b UNIVERSITY OF EDINBURGH   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ENHANCED GREEN FLUORESCENT PROTEIN; GREEN FLUORESCENT PROTEIN; MESSENGER RNA; UNCLASSIFIED DRUG;

ARTICLE; CELL CULTURE; CULTURE MEDIUM; CULTURE OPTIMIZATION; ELECTROPORATION; EMBRYO CELL; GENE EXPRESSION; GENE TRANSFER; HUMAN; HUMAN CELL; PRIORITY JOURNAL; RNA TRANSCRIPTION; STEM CELL; TRANSGENE;

CELL SURVIVAL; CELLS, CULTURED; ELECTROPORATION; FLOW CYTOMETRY; GREEN FLUORESCENT PROTEINS; HUMANS; STEM CELLS; TRANSFECTION;

EID: 6344293929     PISSN: 15362302     EISSN: None     Source Type: Journal    
DOI: 10.1089/clo.2004.6.211     Document Type: Article

References (7)

1. Pfeifer, A., Ikawa, M., Dayn, Y., et al. (2002). Transgenesis by lentiviral vectors: lack of gene silencing in mammalian embryonic stem cells and preimplantation embryos. Proc. Natl. Acad. Sci. USA 99, 2140-2145.
2. Ponsaerts, P., Van Tendeloo, V.F., Cools, N., et al. (2002). mRNA electroporated mature dendritic cells retain transgene expression, phenotypical properties and stimulatory capacity after cryopreservation. Leukemia 16, 1324-1330.
3. Ponsaerts, P., Van Tendeloo, V.F.I., and Berneman, Z.N. (2003). Cancer immunotherapy using RNA-loaded dendritic cells. Clin. Exp. Immunol. 134, 378-84.
4. Reubinoff, B.E., Pera, M.F., Fong, C.Y., et al. (2000). Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro. Nat. Biotechnol. 18, 399-404.
5. Smith-Arica, J.R., Thomson, A.J., Ansell, R., et al. (2003). Infection efficiency of human and mouse embryonic stem cells using adenoviral and adeno-associated viral vectors. Cloning Stem Cells 5, 51-62.
6. Thomson, J.A., Itskovitz-Eldor, J., Shapiro, S.S., et al. (1998). Embryonic stem cell lines derived from human blastocysts. Science 282, 1145-1147.
7. Van Tendeloo, V.F., Ponsaerts, P., Lardon, F., et al. (2001). Highly efficient gene delivery by mRNA electroporation in human hematopoietic cells: superiority to lipofection and passive pulsing of mRNA and to electroporation of plasmid cDNA for tumor antigen loading of dendritic cells. Blood 98, 49-56.