Experimental indication for the existence of multiple Trp rotamers in von willebrand factor A3 domain

Proteins: Structure, Function and Genetics

Volumn 57, Issue 3, 2004, Pages 596-601

  Hellings, Mario ^a   Engelborghs, Yves ^a   Deckmyn, Hans ^a   Vanhoorelbeke, Karen ^a   Schiphorst, Marion E ^b   Akkerman, Jan Willem N ^b   De Maeyer, Marc ^a  

^a UNIVERSITY OF LEUVEN   (Belgium)

^b UNIVERSITY MEDICAL CENTER UTRECHT   (Netherlands)

Author keywords

Fluorescence anisotropy; Fluorescence life time; Thrombosis; Tryptophan rotamers; Von Willebrand factor

Indexed keywords

VON WILLEBRAND FACTOR;

ANALYTIC METHOD; ANISOTROPY; ARTICLE; FLUORESCENCE; MOLECULAR MODEL; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN CONFORMATION; PROTEIN DOMAIN; PROTEIN STRUCTURE; X RAY ANALYSIS;

BINDING SITES; CRYSTALLOGRAPHY, X-RAY; FLUORESCENCE POLARIZATION; HUMANS; MODELS, MOLECULAR; PROTEIN STRUCTURE, TERTIARY; SOLVENTS; TRYPTOPHAN; VON WILLEBRAND FACTOR;

EID: 6344231649     PISSN: 08873585     EISSN: None     Source Type: Journal    
DOI: 10.1002/prot.20227     Document Type: Article

References (20)

1. Huizinga EG, Van der Plas MR, Kroon J, Sixma JJ, Gros P. Crystal structure of the A3 domain of human von Willebrand factor: implications for collagen binding. Structure 1997;5:1147-1156.
2. Romijn RA, Westein E, Bouma B, Schiphorst ME, Sixma JJ, Lenting PJ, Huizinga EG. Mapping the collagen-binding site in the von Willebrand factor-A3 domain. J Biol Chem 2003;278:15035-15039.
3. Wu D, Vanhoorelbeke K, Cauwenberghs N, Meiring M, Depraetere H, Kotze HF, Deckmyn H. Inhibition of the von Willebrand (VWF)-collagen interaction by an antihuman VWF monoclonal antibody results in abolition of in vivo arterial platelet thrombus formation in baboons. Blood 2002;99:3623-3628.
4. Vanhoorelbeke K, Depraetere H, Romijn RA, Huizinga EG, De Maeyer M, Deckmyn H. A consensus tetra-peptide selected by phage display adopts the conformation of a dominant discontinuous epitope of a monoclonal anti-VWF antibody that inhibits the VWF-collagen interaction. J Biol Chem 2003;278:37815-37821.
5. Hellings M, De Maeyer M, Verheyden S, Hao Q, Van Damme EJM, Peumans WJ, Engelborghs Y. The dead-end elimination method, tryptophan rotamers, and fluorescence lifetimes. Biophys J 2003;85:1894-1902.
6. Sillen A, Díaz JF, Engelborghs Y. A step toward the prediction of the fluorescence lifetimes of tryptophan residues in proteins based on structural and spectral data. Protein Science 2000;9:158-169.
7. Bevington PR. Gradient-expansion algorithm. In Data reduction and error analyses for the physical sciences. New York: McGrawHill; 1969. p 235-240.
8. Beechem JM, Knutson JR, Ross JBA, Turner BW, Brand L. Global resolution of heterogeneuos decay by phase modulation fluorometry: mixtures and proteins. Biochemistry 1983;22:6054-6058.
9. More JJ, Sorensen DC. Computing a trust region step. SIAM J Sci Stat Comput 1983;4:553-572.
10. Parker CA, Rees WT. Corrections of fluorescence spectra and the measurement of fluorescence quantum efficiency. Analyst 1960;85:587-600.
11. Kirby EP, Steiner RFS. The influence of solvent and temperature upon the fluorescence of indole derivatives. J Phys Chem 1970;74:4480-4490.
12. Sillen A, Engelborghs Y. The correct use of "average" fluorescence parameters. Photochem Photobiol 1998;67:475-486.
13. Weber G. Theory of differential phase fluorometry: detection of anisotropic molecular rotations. J Phys Chem 1977;66:4081-4091.
14. Lakowicz JE, Laczko G, Gryczinski I. 2-GHz frequency-domain fluorometer. Rev Sci Instrum 1985;57:2499-2506.
15. Lakowicz JR. Principles of fluorescence spectroscopy. New York: Kluwer Academic/Plenum Press; 1999. p 238-264.
16. De Maeyer M, Desmet J, Lasters I. The dead-end elimination theorem: mathematical aspects, implementation, optimizations, evaluation, and performance. Methods Mol Biol 2000;143:265-304.
17. De Maeyer M, Desmet J, Lasters I. All in one: a highly detailed rotamer library improves both the accuracy and speed in the modelling of sidechains by dead-end elimination. Fold Des 1997;2:53-66.
18. Delhaise P, Bardiaux M, De Maeyer M, Prevost M, Vanbelle D, Donneux J, Lasters I, Vancustem E, Alard P, Wodak SJ. The BRUGEL package: toward computer-aided-design of macromolecules. J Mol Graph 1988;6:219-219.
19. Alard P, Wodak SJ. Detection of cavities in a set of interpenetrating spheres. J Comp Chem 1991;12:918-922.
20. Szabo AG, Rayner DM. Fluorescence decay of tryptophan conformers in aqueous solution. J Am Chem Soc USA 1980;102:554-563.