Antiviral immunity in Drosophila requires systemic RNA interference spread

Nature

Volumn 458, Issue 7236, 2009, Pages 346-350

  Saleh, Maria Carla ^a,b   Tassetto, Michel ^a   Van Rij, Ronald P ^a,d   Goic, Bertsy ^b   Gausson, Valérie ^b   Berry, Bassam ^c   Jacquier, Caroline ^c   Antoniewski, Christophe ^c   Andino, Raul ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b INSTITUT PASTEUR   (France)

^c CNRS   (France)

^d RADBOUD UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

DOUBLE STRANDED RNA; GREEN FLUORESCENT PROTEIN; VIRUS RNA;

DEFENSE MECHANISM; FLY; IMMUNITY; RNA; VIRAL DISEASE; VIRUS;

ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; DROSOPHILA MELANOGASTER; FEMALE; IMMUNE RESPONSE; IMMUNE SYSTEM; IMMUNITY; MUTANT; NONHUMAN; PRIORITY JOURNAL; RNA INTERFERENCE; SEQUENCE ANALYSIS; SINDBIS VIRUS; VIRUS TITRATION;

ANIMALS; CELL LINE; DROSOPHILA MELANOGASTER; MICROCOCCUS LUTEUS; PECTOBACTERIUM CAROTOVORUM; RNA INTERFERENCE; RNA VIRUSES; RNA, DOUBLE-STRANDED; SINDBIS VIRUS; SUBSTRATE SPECIFICITY;

DROSOPHILA C VIRUS; DROSOPHILA MELANOGASTER; HEXAPODA; SINDBIS VIRUS; VERTEBRATA;

EID: 62649144073     PISSN: 00280836     EISSN: 14764687     Source Type: Journal    
DOI: 10.1038/nature07712     Document Type: Article

References (24)

1. Baulcombe, D. RNA silencing in plants. Nature 431, 356-363 (2004).
2. Dorner, T. & Radbruch, A. Antibodies and B cell memory in viral immunity. Immunity 27, 384-392 (2007).
3. Roitt, I., Brostoff, J. & Male, D. Immunology (Mosby, 2001).
4. Voinnet, O. Non-cell autonomous RNA silencing. FEBS Lett. 579, 5858-5871 (2005).
5. Galiana-Arnoux, D., Dostert, C., Schneemann, A., Hoffmann, J. A. & Imler, J. L. Essential function in vivo for Dicer-2 in host defense against RNA viruses in Drosophila. Nature Immunol. 7, 590-597 (2006).
6. van Rij, R. P. et al. The RNA silencing endonuclease Argonaute 2 mediates specific antiviral immunity in Drosophila melanogaster. Genes Dev. 20, 2985-2995 (2006).
7. Wang, X. H. et al. RNA interference directs innate immunity against viruses in adult Drosophila. Science 312, 452-454 (2006).
8. Roignant, J. Y. et al. Absence of transitive and systemic pathways allows cell-specific and isoform-specific RNAi in Drosophila. RNA 9, 299-308 (2003).
9. Saleh, M. C. et al. The endocytic pathway mediates cell entry of dsRNA to induce RNAi silencing. Nature Cell Biol. 8, 793-802 (2006).
10. Ulvila, J. et al. Double-stranded RNA is internalized by scavenger receptor-mediated endocytosis in Drosophila S2 cells. J. Biol. Chem. 281, 14370-14375 (2006).
11. Vaistij, F. E., Jones, L. & Baulcombe, D. C. Spreading of RNA targeting and DNA methylation in RNA silencing requires transcription of the target gene and a putative RNA-dependent RNA polymerase. Plant Cell 14, 857-867 (2002).
12. Sijen, T. et al. On the role of RNA amplification in dsRNA-triggered gene silencing. Cell 107, 465-476 (2001).
13. Schubiger, M., Carre, C., Antoniewski, C. & Truman, J. W. Ligand-dependent derepression via EcR/USP acts as a gate to coordinate the differentiation of sensory neurons in the Drosophila wing. Development 132, 5239-5248 (2005).
14. Lee, Y. S. et al. Distinct roles for Drosophila Dicer-1 and Dicer-2 in the siRNA/miRNA silencing pathways. Cell 117, 69-81 (2004).
15. Kennerdell, J. R. & Carthew, R. W. Use of dsRNA-mediated genetic interference to demonstrate that frizzled and frizzled 2 act in the wingless pathway. Cell 95, 1017-1026 (1998).
16. Hoffmann, J. A. The immune response of Drosophila. Nature 426, 33-38 (2003).
17. Dostert, C. et al. The Jak-STAT signaling pathway is required but not sufficient for the antiviral response of Drosophila. Nature Immunol. 6, 946-953 (2005).
18. Porter, J. A., Minke, B. & Montell, C. Calmodulin binding to Drosophila NinaC required for termination of phototransduction. EMBO J. 14, 4450-4459 (1995).
19. Brennecke, J., Hipfner, D. R., Stark, A., Russell, R. B. & Cohen, S. M. bantam encodes a developmentally regulated microRNA that controls cell proliferation and regulates the proapoptotic gene hid in Drosophila. Cell 113, 25-36 (2003).
20. Hahn, C. S., Hahn, Y. S., Braciale, T. J. & Rice, C. M. Infectious Sindbis virus transient expression vectors for studying antigen processing and presentation. Proc. Natl Acad. Sci. USA 89, 2679-2683 (1992).
21. Reed, L. J. & Muench, H. A simple method of estimating fifty percent endpoints. Am. J. Hyg. 27, 493-497 (1938).
22. Okamura, K., Ishizuka, A., Siomi, H. & Siomi, M. C. Distinct roles for Argonaute proteins in small RNA-directed RNA cleavage pathways. Genes Dev. 18, 1655-1666 (2004).
23. Cherry, S. & Perrimon, N. Entry is a rate-limiting step for viral infection in a Drosophila melanogaster model of pathogenesis. Nature Immunol. 5, 81-87 (2004).
24. Goto, A., Blandin, S., Royet, J., Reichhart, J. M. & Levashina, E. A. Silencing of Toll pathway components by direct injection of double-stranded RNA into Drosophila adult flies. Nucleic Acids Res. 31, 6619-6623 (2003).