Treatment with a growth factor-protein mixture inhibits formation of mineralized nodules in osteogenic cell cultures grown on titanium

Journal of Histochemistry and Cytochemistry

Volumn 57, Issue 3, 2009, Pages 265-276

  De Oliva, Marcos Andrade ^a   Maximiano, William Marcatti Amarú ^a   De Castro, Larissa Moreira Spínola ^a   Da Silva Jr , Paulo Eliandro ^a   Fernandes, Roger Rodrigo ^a   Ciancaglini, Pietro ^a   Beloti, Márcio Mateus ^a   Nanci, Antonio ^b   Rosa, Adalberto Luiz ^a   De Oliveira, Paulo Tambasco ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

^b UNIVERSITÉ DE MONTRÉAL   (Canada)

Author keywords

Cell culture; Cell differentiation; Cell proliferation; Growth factors; Mineralization; Osteoblasts; Titanium

Indexed keywords

ALBUMIN; ALIZARIN RED S; ALKALINE PHOSPHATASE; CALCIUM; FIBRONECTIN; GROWTH FACTOR; PLATELET DERIVED GROWTH FACTOR BB; THROMBOSPONDIN; TITANIUM; TRANSFORMING GROWTH FACTOR BETA1; TRANSFORMING GROWTH FACTOR BETA2; PLATELET DERIVED GROWTH FACTOR; SERUM ALBUMIN; SIGNAL PEPTIDE;

ALVEOLAR BONE; ANIMAL CELL; ARTICLE; BONE DEFECT; BONE GROWTH; BONE MINERALIZATION; CALVARIA; CELL DIFFERENTIATION; CONTROLLED STUDY; ENZYME ACTIVITY; HUMAN; HUMAN CELL; IMPLANT; IN VITRO SELECTION; NONHUMAN; OSTEOBLAST; PHENOTYPE; PRIORITY JOURNAL; RAT; THROMBOCYTE RICH PLASMA; ANIMAL; BONE; CELL CULTURE; CYTOLOGY; DOSE RESPONSE; DRUG EFFECT; SCANNING ELECTRON MICROSCOPY; TIME; TISSUE ENGINEERING;

RATTUS;

ANIMALS; BONE AND BONES; CALCIFICATION, PHYSIOLOGIC; CELLS, CULTURED; DOSE-RESPONSE RELATIONSHIP, DRUG; FIBRONECTINS; HUMANS; INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS; MICROSCOPY, ELECTRON, SCANNING; OSTEOBLASTS; PLATELET-DERIVED GROWTH FACTOR; PLATELET-RICH PLASMA; RATS; SERUM ALBUMIN; THROMBOSPONDINS; TIME FACTORS; TISSUE ENGINEERING; TITANIUM; TRANSFORMING GROWTH FACTOR BETA1; TRANSFORMING GROWTH FACTOR BETA2;

EID: 62649101870     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1369/jhc.2008.952713     Document Type: Article

References (61)

1. Anitua E, Sánchez M, Orive G, Andia I (2007) The potential impact of the preparation rich in growth factors (PRGF) in different medical fields. Biomaterials 28:4551-4560
2. Baht GS, Hunter GK, Goldberg HA (2008) Bone sialoprotein-collagen interaction promotes hydroxyapatite nucleation. Matrix Biol 27:600-608
3. Bellows CG, Aubin JE (1989) Determination of numbers of osteoprogenitors present in isolated fetal rat calvaria cells in vitro. Dev Biol 133:8-13
4. Bellows CG, Heersche JN, Aubin JE (1990) Determination of the capacity for proliferation and differentiation of osteoprogenitor cells in the presence and absence of dexamethasone. Dev Biol 140:132-138
5. Beloti MM, Oliveira PT, Gimenes R, Zaghete MA, Bertolini MJ, Rosa AL (2006) In vitro biocompatibility of a novel membrane of the composite poly(vinylidene-trifluoroethylene)/barium titanate. J Biomed Mater Res A 79:282-288
6. Bessho K, Carnes DL, Cavin R, Chen HY, Ong JL (1999) BMP stimulation of bone response adjacent to titanium implants in vivo. Clin Oral Implants Res 10:212-218
7. Bolander ME (1992) Regulation of fracture repair by growth factors. Proc Soc Exp Biol Med 200:165-170
8. Borzini P, Mazzucco L (2005) Platelet gels and releasates. Curr Opin Hematol 12:473-479
9. Borzini P, Mazzucco L (2007) Platelet-rich plasma (PRP) and platelet derivatives for topical therapy. What is true from the biologic view point? ISBT Science Series 2:272-281
10. Borzini P, Mazzucco L, Giampaolo A, Hassan HJ (2006) Platelet gel: the Italian way: a call for procedure standardization and quality control. Transfus Med 16:303-304
11. Choi BH, Zhu SJ, Kim BY, Huh JY, Lee SH, Jung JH (2005) Effect of platelet-rich plasma (PRP) concentration on the viability and proliferation of alveolar bone cells: an in vitro study. Int J Oral Maxillofac Surg 34:420-424
12. Clokie CM, Bell RC (2003) Recombinant human transforming growth factor beta-1 and its effects on osseointegration. J Craniofac Surg 14:268-277
13. de Oliveira PT, de Oliva MA, Maximiano WM, Sebastião KE, Crippa GE, Ciancaglini P, Beloti MM, et al. (2008) Effects of a mixture of growth factors and proteins on the development of the osteogenic phenotype in human alveolar bone cell cultures. J Histochem Cytochem 56:629-638
14. de Oliveira PT, Nanci A (2004) Nanotexturing of titanium-based surfaces upregulates expression of bone sialoprotein and osteopontin by cultured osteogenic cells. Biomaterials 25:403-413
15. de Oliveira PT, Zalzal SF, Beloti MM, Rosa AL, Nanci A (2007) Enhancement of in vitro osteogenesis on titanium by chemically produced nanotopography. J Biomed Mater Res A 80:554-564
16. De Ranieri A, Virdi AS, Kuroda S, Shott S, Leven RM, Hallab NJ, Sumner DR (2005) Local application of rhTGF-beta2 enhances peri-implant bone volume and bone-implant contact in a rat model. Bone 37:55-62
17. Dugrillon A, Eichler H, Kern S, Kluter H (2002) Autologous concentrated platelet-rich plasma (cPRP) for local application in bone regeneration. Int J Oral Maxillofac Surg 31:615-619
18. Ferreira CF, Gomes MCC, Filho JS, Granjeiro JM, Simões CMO, Magini RS (2005) Platelet-rich plasma influence on human osteoblasts growth. Clin Oral Implants Res 16:456-460
19. Fuerst G, Gruber R, Tangl S, Sanroman F, Watzek G (2003) Enhanced bone-to-implant contact by platelet-released growth factors in mandibular cortical bone: a histomorphometric study in minipigs. Int J Oral Maxillofac Implants 18:685-690
20. Gerard D, Carlson ER, Gotcher JE, Jacobs M (2006) Effects of platelet-rich plasma on the healing of autologous bone grafted mandibular defects in dogs. J Oral Maxillofac Surg 64: 443-451
21. Graziani F, Ivanovski S, Cei S, Ducci F, Tonetti M, Gabriele M (2006) The in vitro effect of different PRP concentrations on osteoblasts and fibroblasts. Clin Oral Implants Res 17:212-219
22. Gruber R, Kandler B, Fischer MB, Watzek G (2006) Osteogenic differentiation induced by bone morphogenetic proteins can be suppressed by platelet-released supernatant in vitro. Clin Oral Implants Res 17:188-193
23. Gruber R, Karreth F, Frommlet F, Fischer MB, Watzek G (2003) Platelets are mitogenic for periosteum-derived cells. J Orthop Res 21:941-948
24. Hall J, Sorensen RG, Wozney JM, Wikesjö UM (2007) Bone formation at rhBMP-2-coated titanium implants in the rat ectopic model. J Clin Periodontol 34:444-451
25. Hokugo A, Ozeki M, Kawakami O, Sugimoto K, Mushimoto K, Morita S, Tabata Y (2005) Augmented bone regeneration activity of platelet-rich plasma by biodegradable gelatin hydrogel. Tissue Eng 11:1224-1233
26. Hokugo A, Sawada Y, Hokugo R, Iwamura H, Kobuchi M, Kambara T, Morita S, et al. (2007) Controlled release of platelet growth factors enhances bone regeneration at rabbit calvaria. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 104:44-48
27. Irie K, Zalzal S, Ozawa H, McKee MD, Nanci A (1998) Morphological and immunocytochemical characterization of primary osteogenic cell cultures derived from fetal rat cranial tissue. Anat Rec 252:554-567
28. Jones AA, Buser D, Schenk R, Wozney J, Cochran DL (2006) The effect of rhBMP-2 around endosseous implants with and without membranes in the canine model. J Periodontol 77:1184-1193
29. Kawase T, Okuda K, Saito Y, Yoshie H (2005) In vitro evidence that the biological effects of platelet-rich plasma on periodontal ligament cells is not mediated solely by constituent transforminggrowth factor-beta or platelet-derived growth factor. J Periodontol 76:760-767
30. Kloen P, Di Paola M, Borens O, Richmond J, Perino G, Helfet DL, Goumans MJ (2003) BMP signaling components are expressed in human fracture callus. Bone 33:362-371
31. Lacoste E, Martineau I, Gagnon G (2003) Platelet concentrates: effects of calcium and thrombin on endothelial cell proliferation and growth factor release. J Periodontol 74:1498-1507
32. Liu Y, Enggist L, Kuffer AF, Buser D, Hunziker EB (2007) The influence of BMP-2 and its mode of delivery on the osteoconductivity of implant surfaces during the early phase of osseointegration. Biomaterials 28:2677-2686
33. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265-275
34. Lucarelli E, Beccheroni A, Donati D, Sangiorgi L, Cenacchi A, Del Vento AM, Meotti C, et al. (2003) Platelet-derived growth factors enhance proliferation of human stromal stem cells. Biomaterials 24:3095-3100
35. Martineau I, Lacoste E, Gagnon G (2004) Effects of calcium and thrombin on growth factor release from platelet concentrates: kinetics and regulation of endothelial cell proliferation. Biomaterials 25:4489-4502
36. Marx RE (2004) Platelet-rich plasma: evidence to support its use. J Oral Maxillofac Surg 62:489-496
37. Marx RE, Carlson ER, Eichstaedt RM, Schimmele SR, Strauss JE, Georgeff KR (1998) Platelet-rich plasma: growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 85:638-646
38. Millán JL (2006) Mammalian Alkaline Phosphatases: From Biology to Applications in Medicine and Biotechnology. Weinhein, Wiley VCH
39. Mintz KP, Grzesik WJ, Midura RJ, Robey PG, Termine JD, Fisher LW (1993) Purification and fragmentation of nondenatured bone sialoprotein: evidence for a cryptic, RGD-resistant cell attachment domain. J Bone Miner Res 8:985-995
40. Mooren RE, Merkx MA, Bronkhorst EM, Jansen JA, Stoelinga PJ (2007) The effect of platelet-rich plasma on early and late bone healing: an experimental study in goats. Int J Oral Maxillofac Surg 36:626-631
41. Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65:55-63
42. Nanci A, Zalzal S, Gotoh Y, McKee MD (1996) Ultrastructural characterization and immunolocalization of osteopontin in rat calvarial osteoblast primary cultures. Microsc Res Tech 33:214-231
43. Nikolidakis D, van den Dolder J, Wolke JG, Jansen JA (2008) Effect of platelet-rich plasma on the early bone formation around Ca-P-coated and non-coated oral implants in cortical bone. Clin Oral Implants Res 19:207-213
44. Ramoshebi LN, Matsaba TN, Teare J, Renton L, Patton J, Ripamonti U (2002) Tissue engineering: TGF-beta superfamily members and delivery systems in bone regeneration. Expert Rev MolMed 4:1-11
45. Ranly DM, Lohmann CH, Andreacchio D, Boyan BD, Schwartz Z (2007) Platelet-rich plasma inhibits demineralized bone matrix-induced bone formation in nude mice. J Bone Joint Surg Am 89:139-147
46. Roussy Y, Bertrand Duchesne MP, Gagnon G (2007) Activation of human platelet-rich plasmas: effect on growth factors release, cell division and in vivo bone formation. Clin Oral Implants Res 18:639-648
47. Sarkar MR, Augat P, Shefelbine SJ, Schorlemmer S, Huber-Lang M, Claes L, Kinzl L, et al. (2006) Bone formation in a long bone defect model using a platelet-rich plasma-loaded collagen scaffold. Biomaterials 27:1817-1823
48. Schliephake H (2002) Bone growth factors in maxillofacial skeletal reconstruction. Int J Oral Maxillofac Surg 31:469-484
49. Scholzen T, Gerdes J (2000) The Ki-67 protein: from the known and the unknown. J Cell Physiol 182:311-322
50. Simão AM, Beloti MM, Rosa AL, de Oliveira PT, Granjeiro JM, Pizauro JM, Ciancaglini P (2007) Culture of osteogenic cells from human alveolar bone: a useful source of alkaline phosphatase. Cell Biol Int 31:1405-1413
51. Stein GS, Lian JB, Stein JL, Van Wijnen AJ, Montecino M (1996) Transcriptional control of osteoblast growth and differentiation. Physiol Rev 76:593-629
52. Thomas DM, Johnson SA, Sims NA, Trivett MK, Slavin JL, Rubin BP, Waring P, et al. (2004) Terminal osteoblast differentiation, mediated by runx2 and p27KIP1, is disrupted in osteosarcoma. J Cell Biol 167:925-934
53. Tomoyasu A, Higashio K, Kanomata K, GotoM, Kodaira K, Serizawa H, Suda T, et al. (2007) Platelet-rich plasma stimulates osteoblastic differentiation in the presence of BMPs. Biochem Biophys Res Commun 361:62-67
54. Tye CE, Hunter GK, Goldberg HA (2005) Identification of the type I collagen-binding domain of bone sialoprotein and characterization of the mechanism of interaction. J Biol Chem 280:13487-13492
55. Tye CE, Rattray KR, Warner KJ, Gordon JA, Sodek J, Hunter GK, Goldberg HA (2003) Delineation of the hydroxyapatite-nucleating domains of bone sialoprotein. J Biol Chem 278:7949-7955
56. Uggeri J, Belletti S, Guizzardi S, Poli T, Cantarelli S, Scandroglio R, Gatti R (2007) Dose-dependent effects of platelet gel releasate on activities of human osteoblasts. J Periodontol 78:1985-1991
57. van den Dolder J, Mooren R, Vloon AP, Stoelinga PJ, Jansen JA (2006) Platelet-rich plasma: quantification of growth factor levels and the effect on growth and differentiation of rat bone marrow cells. Tissue Eng 12:3067-3073
58. van den Dolder J, Spauwen PH, Jansen JA (2003) Evaluation of various seeding techniques for culturing osteogenic cells on titanium fiber mesh. Tissue Eng 9:315-325
59. Venne D, Raymond J, Allas S, Roy D, Leclerc G, Boushira M, Brazeau P (1999) Healing of experimental aneurysms. II: platelet extracts can increase the thickness of the neointima at the neck of treated aneurysms. J Neuroradiol 26:92-100
60. Weibrich G, Hansen T, Kleis W, Buch R, Hitzler WE (2004) Effect of platelet concentration in platelet-rich plasma on peri-implant bone regeneration. Bone 34:665-671
61. Wennberg C, Hessle L, Lundberg P, Mauro S, Narisawa S, Lerner UH, Millán JL (2000) Functional characterization of osteoblasts and osteoclasts from alkaline phosphatase knockout mice. J Bone Miner Res 15:1879-1888