A two-host fosmid system for functional screening of (meta)genomic libraries from extreme thermophiles

Systematic and Applied Microbiology

Volumn 32, Issue 3, 2009, Pages 177-185

  Angelov, Angel ^a,b   Mientus, Markus ^b   Liebl, Susanne ^a,b   Liebl, Wolfgang ^b  

^a UNIVERSITY OF GÖTTINGEN   (Germany)

^b TECHNICAL UNIVERSITY OF MUNICH   (Germany)

Author keywords

Functional screening; Metagenomics; Thermophile; Xylanase

Indexed keywords

GENOMIC DNA; XYLAN ENDO 1,3 BETA XYLOSIDASE;

ARTICLE; BACTERIAL GENE; CHROMOSOME INSERTION; CLONE; DNA LIBRARY; ESCHERICHIA COLI; FOSMID LIBRARY; GENE EXPRESSION; GENE ISOLATION; GENE LOCUS; GENETIC RECOMBINATION; GENETIC SCREENING; METAGENOMICS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PYR GENE; SPIROCHAETA THERMOPHILA; THERMOPHILIC BACTERIUM; THERMUS THERMOPHILUS;

CLONING, MOLECULAR; ENDO-1,4-BETA XYLANASES; ESCHERICHIA COLI; GENETIC VECTORS; GENOMIC LIBRARY; GENOMICS; HOT TEMPERATURE; RECOMBINATION, GENETIC; SPIROCHAETA; THERMUS THERMOPHILUS; TRANSFORMATION, BACTERIAL;

ESCHERICHIA COLI; SPIROCHAETA; SPIROCHAETA THERMOPHILA; THERMUS THERMOPHILUS;

EID: 62649087889     PISSN: 07232020     EISSN: 16180984     Source Type: Journal    
DOI: 10.1016/j.syapm.2008.01.003     Document Type: Article

References (26)

1. Aksenova H.Y., Rainey F.A., Janssen P.H., Zavarzin G.A., and Morgan H.W. Spirochaeta thermophila sp. nov., an obligately anaerobic, polysaccharolytic, extremely thermophilic bacterium. Int. J. Syst. Bacteriol. 42 (1992) 175-177
2. Berkner S., Grogan D., Albers S.V., and Lipps G. Small multicopy, non-integrative shuttle vectors based on the plasmid pRN1 for Sulfolobus acidocaldarius and Sulfolobus solfataricus, model organisms of the (cren-)archaea. Nucleic Acids Res. 35 (2007) e88
3. Courtois S., Cappellano C.M., Ball M., Francou F.X., Normand P., Helynck G., Martinez A., Kolvek S.J., Hopke J., Osburne M.S., August P.R., Nalin R., Guerineau M., Jeannin P., Simonet P., and Pernodet J.L. Recombinant environmental libraries provide access to microbial diversity for drug discovery from natural products. Appl. Environ. Microbiol. 69 (2003) 49-55
4. de Grado M., Castan P., and Berenguer J. A high-transformation-efficiency cloning vector for Thermus thermophilus. Plasmid 42 (1999) 241-245
5. Degryse E., Glansdorff N., and Pierard A. A comparative analysis of extreme thermophilic bacteria belonging to the genus Thermus. Arch. Microbiol. 117 (1978) 189-196
6. Gabor E.M., Alkema W.B., and Janssen D.B. Quantifying the accessibility of the metagenome by random expression cloning techniques. Environ. Microbiol. 6 (2004) 879-886
7. Heiner C.R., Hunkapiller K.L., Chen S.M., Glass J.I., and Chen E.Y. Sequencing multimegabase-template DNA with BigDye terminator chemistry. Genome Res. 8 (1998) 557-561
8. Henne A., Bruggemann H., Raasch C., Wiezer A., Hartsch T., Liesegang H., Johann A., Lienard T., Gohl O., Martinez-Arias R., Jacobi C., Starkuviene V., Schlenczeck S., Dencker S., Huber R., Klenk H.P., Kramer W., Merkl R., Gottschalk G., and Fritz H.J. The genome sequence of the extreme thermophile Thermus thermophilus. Nat. Biotechnol. 22 (2004) 547-553
9. Hidaka Y., Hasegawa M., Nakahara T., and Hoshino T. The entire population of Thermus thermophilus cells is always competent at any growth phase. Biosci. Biotechnol. Biochem. 58 (1994) 1338-1339
10. Jonuscheit M., Martusewitsch E., Stedman K.M., and Schleper C. A reporter gene system for the hyperthermophilic archaeon Sulfolobus solfataricus based on a selectable and integrative shuttle vector. Mol. Microbiol. 48 (2003) 1241-1252
11. Koyama Y., and Furukawa K. Cloning and sequence analysis of tryptophan synthetase genes of an extreme thermophile, Thermus thermophilus HB27: plasmid transfer from replica-plated Escherichia coli recombinant colonies to competent T. thermophilus cells. J. Bacteriol. 172 (1990) 3490-3495
12. Koyama Y., Hoshino T., Tomizuka N., and Furukawa K. Genetic transformation of the extreme thermophile Thermus thermophilus and of other Thermus spp. J. Bacteriol. 166 (1986) 338-340
13. Lasa I., de Grado M., de Pedro M.A., and Berenguer J. Development of Thermus-Escherichia shuttle vectors and their use for expression of the Clostridium thermocellum celA gene in Thermus thermophilus. J. Bacteriol. 174 (1992) 6424-6431
14. Liebl W. Genomics taken to the extreme. Nat. Biotechnol. 22 (2004) 524-525
15. Martinez A., Kolvek S.J., Yip C.L., Hopke J., Brown K.A., MacNeil I.A., and Osburne M.S. Genetically modified bacterial strains and novel bacterial artificial chromosome shuttle vectors for constructing environmental libraries and detecting heterologous natural products in multiple expression hosts. Appl. Environ. Microbiol. 70 (2004) 2452-2463
16. Omelchenko M.V., Wolf Y.I., Gaidamakova E.K., Matrosova V.Y., Vasilenko A., Zhai M., Daly M.J., Koonin E.V., and Makarova K.S. Comparative genomics of Thermus thermophilus and Deinococcus radiodurans: divergent routes of adaptation to thermophily and radiation resistance. BMC Evol. Biol. 5 (2005) 57
17. Oshima T., and Imahori K. Description of Thermus thermophilus comb. nov., a nonsporulating thermophilic bacterium from a Japanese thermal spa. Int. J. Syst. Bacteriol. 24 (1974) 102-112
18. Park H.S., Kayser K.J., Kwak J.H., and Kilbane II J.J. Heterologous gene expression in Thermus thermophilus: beta-galactosidase, dibenzothiophene monooxygenase, PNB carboxy esterase, 2-aminobiphenyl-2,3-diol dioxygenase, and chloramphenicol acetyl transferase. J. Ind. Microbiol. Biotechnol. 31 (2004) 189-197
19. Park H.S., and Kilbane II J.J. Gene expression studies of Thermus thermophilus promoters PdnaK, Parg and Pscs-mdh. Lett. Appl. Microbiol. 38 (2004) 415-422
20. Sambrook J., Fritsch E.F., and Maniatis T. Molecular Cloning: a Laboratory Manual. second ed. (1989), Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
21. Schwarzenlander C., and Averhoff B. Characterization of DNA transport in the thermophilic bacterium Thermus thermophilus HB27. FEBS J. 273 (2006) 4210-4218
22. Tamakoshi M., Uchida M., Tanabe K., Fukuyama S., Yamagishi A., and Oshima T. A new Thermus-Escherichia coli shuttle integration vector system. J. Bacteriol. 179 (1997) 4811-4814
23. Ten L.N., Im W.T., Kim M.K., Kang M.S., and Lee S.T. Development of a plate technique for screening of polysaccharide-degrading microorganisms by using a mixture of insoluble chromogenic substrates. J. Microbiol. Methods 56 (2004) 375-382
24. Weisburg W.G., Giovannoni S.J., and Woese C.R. The Deinococcus-Thermus phylum and the effect of rRNA composition on phylogenetic tree construction. Syst. Appl. Microbiol. 11 (1989) 128-134
25. Wild J., Hradecna Z., and Szybalski W. Conditionally amplifiable BACs: switching from single-copy to high-copy vectors and genomic clones. Genome Res. 12 (2002) 1434-1444
26. Winterhalter C., and Liebl W. Two extremely thermostable xylanases of the hyperthermophilic bacterium Thermotoga maritima MSB8. Appl. Environ. Microbiol. 61 (1995) 1810-1815