Fibroblast-derived induced pluripotent stem cells show no common retroviral vector insertions

Stem Cells

Volumn 27, Issue 2, 2009, Pages 300-306

  Vakas, Florencio ^a   Stadtfeld, Matthias ^b   De Andres Aguayo, Luisa ^a   Maherali, Nimet ^b   Di Tullio, Alessandro ^a   Pantano, Lorena ^a   Notredame, Cedric ^a   Hochedlinger, Konrad ^b,d   Graf, Thomas ^a,c  

^a CENTRE FOR GENOMIC REGULATION CRG   (Spain)

^b MASSACHUSETTS GENERAL HOSPITAL CANCER CENTER   (United States)

^c INSTITUCIÓ CATALANA DE RECERCA I ESTUDIS AVANÇATS ICREA   (Spain)

^d MASSACHUSETTS GENERAL HOSPITAL   (United States)

Author keywords

Cellular reprogramming; Induced pluripotent stem cells; Insertional mutagenesis; Retroviral insertions; Retroviral tagged mouse genes

Indexed keywords

KRUPPEL LIKE FACTOR 4; OCTAMER TRANSCRIPTION FACTOR 4; RETROVIRUS VECTOR; TRANSCRIPTION FACTOR SOX2;

ANIMAL CELL; ARTICLE; BIOINFORMATICS; CELL CLONE; CELL DIFFERENTIATION; EMBRYO; FIBROBLAST; GENE EXPRESSION REGULATION; GENE INSERTION; GENE MAPPING; GENETIC TRANSCRIPTION; MOUSE; NONHUMAN; ONCOGENE C MYC; PLURIPOTENT STEM CELL; POLYMERASE CHAIN REACTION; TRANSCRIPTION INITIATION SITE;

ANIMALS; BLOTTING, SOUTHERN; CELL LINE; COMPUTATIONAL BIOLOGY; FIBROBLASTS; GENETIC VECTORS; HUMANS; KRUPPEL-LIKE TRANSCRIPTION FACTORS; MICE; OCTAMER TRANSCRIPTION FACTOR-3; PLURIPOTENT STEM CELLS; POLYMERASE CHAIN REACTION; PROTO-ONCOGENE PROTEINS C-MYC; RETROVIRIDAE; SOXB1 TRANSCRIPTION FACTORS;

IPS;

EID: 62549094908     PISSN: 10665099     EISSN: None     Source Type: Journal    
DOI: 10.1634/stemcells.2008-0696     Document Type: Article

References (21)

1. Okita K, Ichisaka T, Yamanaka S. Generation of germline-competent induced pluripotent stem cells. Nature 2007;448:313-317.
2. Wernig M, Meissner A, Foreman R et al. In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state. Nature 2007;448: 318-324.
3. Maherali N, Sridharan R, Xie W et al. Directly reprogrammed fibroblasts show global epigenetic remodeling and widespread tissue contribution. Cell Stem Cell 2007;1:55-70.
4. Takabasbi K, Tanabe K, Ohnuki M et al. Induction of pluripotent stem cells from adult human fibroblasts bv defined factors. Cell 2007:131: 861-872.
5. Yamanaka S. Strategies and new developments in the generation of patient-speeific pluripotent stem cells. Cell Stem Cell 2007;1:39-49.
6. Wu X, Li Y, Crise B et al. Transcription start regions in the human genome are favored targets for MLV integration. Science 2003;300: 1749-1751.
7. Mitchell RS? Beitzel BP, Schroder AR et al. Retroviral DNA integration: ASLV. HIV, and Mlv show distinct target site preferences. PLoS Biol 2004;2:E234.
8. Mikkers H. Berns A. Retroviral insertional mutagenesis: Tagging cancer pathways. Adv Cancer Res 2003;88:53-99.
9. Akagi K, Suzuki T, Stephens RM et al. RTCGD: Retroviral tagged cancer gene database. Nucleic Acids Res 2004;32:D523-D527.
10. Kustikova O, Fehse B, Modlich U et al Clonal dominance of hematopoietic stem cells triggered by retroviral gene marking. Science 2005; 308:1171-1174.
11. Kustikova OS, Geiger H. Li Z et al. Retroviral vector insertion sites associated with dominant hematopoietic clones mark "stemness" pathways. Blood 2007;109:1897-1907.
12. Aoi T, Yae K, Nakagawa M et al. Generation of pluripotent stem cells from adult mouse liver and stomach cells. Science 2008;321:699-702.
13. Schmidt M, Hoffmann G, Wissler M el al. Detection and direct genomic sequencing of multiple rare unknown flanking DNA in highly complex samples. Hum Gene Ther 2001;12:743-749.
14. Wang GP, Garrigue A, Ciuffi A et al. DNA bar coding and pyrosequencing to analyze adverse events in therapeutic gene transfer. Nucleic Acids Res 2008;36:e49.
15. Hawley RG. Does retroviral insertional mutagenesis play a role in the generation of induced pluripotent stem cells? Mol Ther 2008; 16: 1354-1355.
16. Stadtfeld M, Brennand K, Hochedlinger K. Reprogramming of pancreatic Beta cells into induced pluripotent stem cells. Curr Biol 2008; 18: 890-894.
17. Hanna J, Markoulaki S, Schorderet P et al. Direct reprogramming of terminally differentiated mature B Iymphocytes to pluripotencv. Cell 2008;133:250-264.
18. Meissner A, Wernig M, Jaenisch R. Direct reprogramming of genetically unmodified fibroblasts into pluripotent stem cells. Nat Biotechnol 2007; 25:1177-1181.
19. Shi Y, Do JT, Desponts C el al. A combined chemical and genetic approach for the generation of induced pluripotent stem cells. Cell Stem Cell 2008;2:525-528.
20. Huangfu D, Maehr R, Guo W et al. Induction of pluripotent stem cells by defined factors is greatly improved by small-molecule compounds. Nat Biotechnol 2008;26:795-797.
21. Mikkelsen TS, Hanna J, Zhang X et al. Dissecting direct reprogramming through integrative genomic analysis. Nature 2008;454:49-55.