Expression of recombinant human FADD, preparation of its polyclonal antiserum and the application in immunoassays

Cellular and Molecular Immunology

Volumn 5, Issue 6, 2008, Pages 471-474

  Marikar, Faiz M M T ^a   Ma, Dingyuan ^a   Ye, Jianqiang ^a   Tang, Bo ^a   Zheng, Weijuan ^a   Zhang, Jing ^a   Lu, Min ^a   Hua, Zichun ^a  

^a NANJING UNIVERSITY   (China)

Author keywords

FADD; His tag fusion protein; immunofluorescence assay; polyclonal antibody

Indexed keywords

ANTISERUM; FAS ASSOCIATED DEATH DOMAIN PROTEIN; RECOMBINANT PROTEIN;

ANIMAL; ARTICLE; CELL LINE; FLUORESCENT ANTIBODY TECHNIQUE; GENETIC TRANSFECTION; HUMAN; IMMUNOASSAY; IMMUNOLOGY; RABBIT;

ANIMALS; CELL LINE; FAS-ASSOCIATED DEATH DOMAIN PROTEIN; FLUORESCENT ANTIBODY TECHNIQUE; HUMANS; IMMUNE SERA; IMMUNOASSAY; RABBITS; RECOMBINANT PROTEINS; TRANSFECTION;

EID: 62449084925     PISSN: 16727681     EISSN: None     Source Type: Journal    
DOI: 10.1038/cmi.2008.59     Document Type: Article

References (14)

1. Nagata S. Apoptosis by death factor. Cell. 1997;88:355-365. (Pubitemid 27131377)
2. Nagata S, Golstein P. The Fas death factor. Science. 1995;267:1449-1456.
3. Osborn SL, Sohn SJ, Winoto A. Constitutive phosphorylation mutation in Fas-associated death domain (FADD) results in early cell cycle defects. J Biol Chem. 2007;282:22786-22792. (Pubitemid 47267372)
4. Boldin MP, Varfolomeev EE, Pancer Z, Mett IL, Camonis JH, Wallach D. A novel protein that interacts with the death domain of Fas/APO1 contains a sequence motif related to the death domain. J Biol Chem. 1995;270:7795-7798.
5. Chinnaiyan AM, O'Rourke K, Tewari M, Dixit VM. FADD, a novel death domain-containing protein, interacts with the death domain of Fas and initiates apoptosis. Cell. 1995;81:505-512.
6. Kischkel FC, Hellbardt S, Behrmann I, et al. Cytotoxicitydependent APO-1 (Fas/CD95) - associated proteins form a death-inducing signaling complex (DISC) with the receptor. EMBO J. 1995;14:5579-5588.
7. Perez D, White E. E1B 19K inhibits Fas-mediated apoptosis through FADD-dependent sequestration of FLICE. J Cell Biol. 1998;141:1255-1266. (Pubitemid 28265618)
8. Mishima K, Nariari Y, Yoshimura Y. Carboplatin induces Fas (APO-1/CD95) dependent apoptosis of human tongue carcinoma cells; sensitization for apoptosis by upregulation of FADD expression. Int J Cancer. 2003;105:593-600. (Pubitemid 36687725)
9. Tourneur L, Delluc S, Levy V, et al. Absence or low expression of fas-associated protein with death domain in acute myeloid leukemia cells predicts resistance to chemotherapy and poor outcome. Cancer Res. 2004;64:8101-8108. (Pubitemid 39446949)
10. Tourneur L, Mistou S, Michiels FM, et al. Loss of FADD protein results in a biased Fas-signaling pathway and correlates with the development of tumoral status in thyroid follicular cells. Oncogene. 2003;22:2795-2804. (Pubitemid 36609593)
11. Ye J, Shao H, Ma D, et al. Development of a monoclonal antibody-based sandwich ELISA for detection of human FADD. Ying Yong Sheng Wu Hua Xue Yu Sheng Wu Ji Shu. 2008;50:143-146.
12. Suzuki A, Araki T, Miura M, Tsutomi Y. Functional absence of FADD in PLC/PRF/5 hepatoma cells: possible involvement in the transformation to helatoma in HBV-infected hepatocytes. Pro Soc Exp Biol Med. 1999;221:72-79.
13. Gajate C, Molinedo F. Cytoskeleton-mediated death and ligand concentration in lipid rafts forms apoptosis-promoting clusters in cancer chemotheraphy. J Biol Chem. 2005;280:11641-11647. (Pubitemid 40418478)
14. Carstens BP, Perry ME. Purification of a fragment of MDM2 for production of polyclonal antisera. Prot Expr Puri. 2001;21:361-365. (Pubitemid 34161461)