Development and validation of an improved RT-PCR assay with nested universal primers for detection of hepatitis E virus strains with significant sequence divergence

Journal of Virological Methods

Volumn 137, Issue 2, 2006, Pages 325-333

  Inoue, Jun ^a   Takahashi, Masaharu ^a   Yazaki, Yasuyuki ^b   Tsuda, Fumio ^c   Okamoto, Hiroaki ^a  

^a JICHI MEDICAL UNIVERSITY   (Japan)

^b Kobayashi Hospital   (Japan)

^c Shinagawa ku   (Japan)

Author keywords

Genotype; Hepatitis E virus; HEV RNA; Phylogenetic tree; RT PCR

Indexed keywords

VIRUS DNA; VIRUS RNA;

ACCURACY; AMPLICON; ARTICLE; CASE REPORT; CONTROLLED STUDY; DILUTION; GENE AMPLIFICATION; GENE SEQUENCE; GENETIC VARIABILITY; GENOTYPE; HEPATITIS E; HEPATITIS E VIRUS; HUMAN; INTERMETHOD COMPARISON; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PHYLOGENY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEQUENCE ALIGNMENT; UNINDEXED SEQUENCE; VALIDATION PROCESS; VIREMIA; VIRUS DETECTION; VIRUS STRAIN;

CONSERVED SEQUENCE; DNA PRIMERS; GENOTYPE; HEPATITIS E; HEPATITIS E VIRUS; HUMANS; MOLECULAR SEQUENCE DATA; PHYLOGENY; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS, DNA; VIRAL PROTEINS; VIREMIA;

HEPATITIS E VIRUS;

EID: 33748679718     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2006.07.004     Document Type: Article

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