Simplified culture techniques for growth and differentiation of murine and human pre-adipocytes for translational applications

Cytotherapy

Volumn 11, Issue 1, 2009, Pages 52-60

  Herrera Herrera, M L ^a   Zapata Bustos, R ^a   Salazar Olivo, L A ^a  

^a INSTITUTO POTOSINO DE INVESTIGACIÓN CIENTÍFICA Y TECNOLÓGICA   (Mexico)

Author keywords

3T3 pre adipocytes; Adipogenesis; Cell proliferation; Human subcutaneous pre adipocytes; L15 medium

Indexed keywords

CARBON DIOXIDE; GLUCOSE TRANSPORTER 4; GLYCEROL 3 PHOSPHATE DEHYDROGENASE; PROTEIN P2;

ADIPOCYTE; ADIPOGENESIS; ANIMAL CELL; ARTICLE; CARBON DIOXIDE FIXATION; CELL CULTURE; CELL DIFFERENTIATION; CELL GROWTH; CELL ISOLATION; COLONY FORMING UNIT; CONTROLLED STUDY; CULTURE MEDIUM; CULTURE TECHNIQUE; ENZYME ACTIVITY; GAS EXCHANGE; HUMAN; HUMAN CELL; LIPID STORAGE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

EID: 62149142769     PISSN: 14653249     EISSN: 14772566     Source Type: Journal    
DOI: 10.1080/14653240802495963     Document Type: Article

References (28)

1. Fraser, JK, Wulur, I. Alfonso, Z. et al. (2006) Fat tissue: An underappreciated source of stem cells for biotechnology. Trends Biotechnol, 24, pp. 150-154.
2. Gimble, JM, Katz, AJ and Bunnell, BA. (2007) Adipose-derived stem cells for regenerative medicine. Circ Res, 100, pp. 1249-1260.
3. Helder, MN, Knippenberg, M. Klein-Nulend, J. et al. (2007) Stem cells from adipose tissue allow challenging new concepts for regenerative medicine. Tissue Eng, 13, pp. 1799-1808.
4. Goh, BC, Thirumala, S. Kilroy, G. et al. (2007) Cryopreservation characteristics of adipose-derived stem cells: Maintenance of differentiation potential and viability. J Tissue Eng Regen Med, 1, pp. 322-324.
5. Parker, AM, Shang, H. Khurgel, M. et al. (2007) Low serum and serum-free culture of multipotential human adipose stem cells. Cytotherapy, 9, pp. 637-646.
6. Suga, H., Shigeura, T. Matsumoto, D. et al. (2007) Rapid expansion of human adipose-derived stromal cells preserving multipotency. Cytotherapy, 9, pp. 738-745.
7. Barngrover, D., Thomas, J. and Thilly, WG. (1985) High density mammalian cell growth in Leibovitz bicarbonate-free medium: Effects of fructose and galactose on culture biochemistry. J Cell Sci, 78, pp. 173-189.
8. Mackenzie, CG, Mackenzie, JB and Beck, P. (1961) The effect of pH on growth, proteins synthesis and lipid rich particles of cultured mammalian cells. J Biophys Biochem Cytol, 9, pp. 141-156.
9. Leibovitz, A. (1963) The growth and maintenance of tissue-cell cultures in free gas exchange with the atmosphere. Am J Hygiene, 78, pp. 173-180.
10. Yang, Y., Chen, X. Ding, F. et al. (2007) Biocompatibility evaluation of silk fibroin with peripheral nerve tissues and cells in vitro. Biomaterials, 28, pp. 1643-1652.
11. Wen, CM, Cheng, YH Huang, YF et al. (2008) Isolation and characterization of a neural progenitor cell line from tilapia brain. Comp Biochem Physiol A Mol Integr Physiol, 49, pp. 167-180.
12. Mitaka, T., Norioka, K. and Mochizuki, Y. (1993) Redifferentiation of proliferated rat hepatocytes cultured in L15 medium supplemented with EGF and DMSO. In Vitro Cell Dev Biol Anim, 29, pp. 714-722.
13. Coundouris, JA, Grant, MH Engeset, J. et al. (1993) Cryopreservation of human adult hepatocytes for use in drug metabolism and toxicity studies. Xenobiotica, 23, pp. 1399-1409.
14. Fujii, M., Yoshino, I. Suzuki, M. et al. (1996) Primary culture of chicken hepatocytes in serum-free medium (pH 7.8) secreted albumin and transferrin for a long period in free gas exchange with atmosphere. Int J Biochem Cell Biol, 28, pp. 1381-1391.
15. Le Marrec-Croq, F, Fritayre, P. Chesné, C. et al. (1998) Cryopreservation of Pecten maximus heart cells. Cryobiology, 37, pp. 200-206.
16. de Jesus Ribeiro, C, Ohara, MT and Gama, P. (2005) Alternative model to human skin organ culture: A preliminary study with Leibovitz L15 medium. Microsc Res Tech, 66, pp. 139-144.
17. de Kreuk, AM, Jonkhoff, AR Zevenbergen, A. et al. (2001) Storage of unprocessed G-CSF-mobilized whole blood in a modified Leibovitz's L15 medium preserves clonogenic capacity for at least 7 days. Bone Marrow Transplant, 28, pp. 145-155.
18. Zuk, PA, Zhu, M. Mizuno, H. et al. (2001) Multilineage cells from human adipose tissue: Implications for cell-based therapies. Tissue Eng, 7, pp. 211-226.
19. Ramirez-Zacarias, JL, Castro-Muñozledo, F. and Kuri-Harcuch, W. (1992) Quantitation of adipose conversion and triglycerides by staining intracytoplasmic lipid with oil red O. Histochemistry, 97, pp. 493-497.
20. Salazar-Olivo, LA, Castro-Muñozledo, F. and Kuri-Harcuch, W. (1995) A preadipose 3T3 cell variant highly sensitive to adipogenic factors and to human growth hormone. J Cell Sci, 108, pp. 2101-2107.
21. Morais, C., Westhuyzen, J. Pat, B. et al. (2005) High ambient glucose is effect neutral on cell death and proliferation in human proximal tubular epithelial cells. Am J Physiol Renal Physiol, 289, p. F401.
22. Hems, DA, Rath, EA and Verrinder, TR. (1975) Fatty acid synthesis in liver and adipose tissue of normal and genetically obese (ob/ob) mice during the 24-hour cycle. Biochem J, 150, pp. 167-173.
23. Aguiari, P., Leo, S. Zavan, B. et al. (2008) High glucose induces adipogenic differentiation of muscle-derived stem cells. Proc Natl Acad Sci USA, 105, pp. 1226-1231.
24. Thompson, KA and Kleinzeller, A. (1989) Accumulation of some hexoses in adipocytes: Properties of the system. Am J Physiol Cell Physiol, 257, p. C214.
25. Green, A., Rumberger, JM Stuart, CA et al. (2004) Stimulation of lipolysis by tumor necrosis factor-a in 3T3-L1 adipocytes is glucose dependent. Implications for long-term regulation of lipolysis. Diabetes, 53, pp. 74-81.
26. Floyd, ZE, Kilroy, G. Wu, X. et al. (2007) Effects of prolyl hydroxylase inhibitors on adipogenesis and hypoxia inducible factor 1 alpha levels under normoxic conditions. J Cell Biochem, 101, pp. 1545-1557.
27. Gentil, C., Le Jan, S Philippe, J. et al. (2006) Is oxygen a key factor in the lipodystrophy phenotype?. Lipids Health Dis, 5, p. 27.
28. Lin, Q., Lee, Y-J and Yun, Z. (2006) Differentiation arrest by hypoxia. J Biol Chem, 281, pp. 30678-30683.