Low serum and serum-free culture of multipotential human adipose stem cells

Cytotherapy

Volumn 9, Issue 7, 2007, Pages 637-646

  Parker, A M ^a   Shang, H ^a   Khurgel, M ^a   Katz, A J ^a  

^a UNIVERSITY OF VIRGINIA   (United States)

Author keywords

Adipose stem cells; Fetal bovine serum; Low serum culture; Media; Serum free culture; Stem cells

Indexed keywords

ALDEHYDE DEHYDROGENASE; CD133 ANTIGEN; CD31 ANTIGEN; CD34 ANTIGEN; HLA ANTIGEN;

ADIPOCYTE; ADIPOSE TISSUE CELL; ADULT; ARTICLE; CARTILAGE CELL; CELL CULTURE; CELL DIFFERENTIATION; CELL GROWTH; CELL POPULATION; CLINICAL ARTICLE; CONTROLLED STUDY; CULTURE MEDIUM; CULTURE TECHNIQUE; FEMALE; HUMAN; HUMAN CELL; HUMAN TISSUE; IN VITRO STUDY; INTERMETHOD COMPARISON; MULTIPOTENT STEM CELL; OSTEOCYTE; PRIORITY JOURNAL; PROTEIN EXPRESSION;

ADIPOCYTES; ADIPOGENESIS; ADIPOSE TISSUE; BIOLOGICAL MARKERS; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL PROLIFERATION; CHONDROGENESIS; CULTURE MEDIA; CULTURE MEDIA, SERUM-FREE; FLOW CYTOMETRY; HUMANS; MULTIPOTENT STEM CELLS; OSTEOGENESIS; SERUM;

EID: 35349004716     PISSN: 14653249     EISSN: 14772566     Source Type: Journal    
DOI: 10.1080/14653240701508452     Document Type: Article

References (19)

1. Peterson, B., Zhang, J. and Iglesias, R. (2005) Healing of critically sized femoral defects, using genetically modified mesenchymal stem cells from human adipose tissue. Tissue Eng, 11, pp. 120-129.
2. Cowan, CM, Shi, Y-Y and Aalami, OO (2004) Adipose-derived adult stromal cells heal critical-size mouse calvarial defects. Nature Biotechnol, 22, pp. 560-567.
3. Lendeckel, S., Jodicke, A. and Christophis, P. (2004) Autologous stem cells (adipose) and fibrin glue used to treat widespread traumatic calvarial defects: Case report. J Cran Max Fac Surg, 32, pp. 370-373.
4. Guilak, F., Awad, HA and Fermor, B. (2004) Adipose-derived adult stem cells for cartilage tissue engineering. Biorheology, 41, pp. 389-399.
5. Fraser, JK, Schreiber, RE, Zuk, PA and Hedrick, MH. (2004) Adult stem cell therapy for the heart. Int J Biochem Cell Biol, 36, pp. 658-666.
6. Pittenger, MF and Martin, BJ. (2004) Mesenchymal stem cells and their potential as cardiac therapeutics. Circ Res, 95, pp. 9-20.
7. Planat-Benard, V., Menard, C. and Andre, M. (2004) Spontaneous cardiomyocyte differentiation from adipose tissue stroma cells. Circ Res, 94, pp. 223-229.
8. Kang, SK, Lee, DH and Bae, YC (2003) Improvement of neurological deficits by intracerebral transplantation of human adipose tissue-derived stromal cells after cerebral ischemia in rats. Exp Neurol, 83, pp. 355-366.
9. Chen, J. and Wang, L. (2001) Therapeutic benefit of intravenous administration of bone marrow stromal cells after cerebral ischemia in rats. Stroke, 32, pp. 1005-1011.
10. Spees, JL, Gregory, CA and Singh, H. (2004) Internalized antigens must be removed to prepare hypoimmunogenic mesenchymal stem cells for cell and gene therapy. Mol Ther J Am Soc Gene Ther, 9, pp. 747-756.
11. Mackensen, A., Drager, R. and Schlesier, M. (2000) Presence of IgE antibodies to bovine serum albumin in a patient developing anaphylaxis after vaccination with human peptide-pulsed dendritic cells. Cancer Immunol Immunother, 49, pp. 152-156.
12. Selvaggi, TA, Walker, RE and Fleisher, TA. (1997) Development of antibodies to fetal calf serum with arthus-like reactions in human immunodeficiency virus-infected patients given syngeneic lymphocyte infusions. Blood, 89, pp. 776-779.
13. Tuschong, L., Soenen, SL and Blaese, RM (2002) Immune response to fetal calf serum by two adenosine deaminase-deficient patients after T cell gene therapy. Hum Gene Ther, 13, pp. 1605-1610.
14. Kim, SJ, Cho, HH and Kim, YJ (2005) Human adipose stromal cells expanded in human serum promote engraftment of human peripheral blood hematopoietic stem cells in NOD/SCID mice. Biochem Biophys Res Comm, 329, pp. 25-31.
15. Zuk, PA, Zhu, M. and Mizuno, H. (2001) Multilineage cells from human adipose tissue: Implications for cell-based therapies. Tissue Eng, 7, pp. 211-228.
16. Katz, AJ. (2002) Methods of Tissue Engineering, pp. 277-286. Academic Press, San Diego
17. Ramirez-Zacarias, JL, Castro-Munozledo, F. and Kuri-Harcuch, W. (1992) Quantitation of adipose conversion and triglycerides by staining intracytoplasmic lipids with Oil red O. Histochemistry, 97, pp. 493-497.
18. Huang, JI, Zuk, PA and Jones, NF (2004) Chondrogenic potential of multipotential cells from human adipose tissue. Plastic Reconstr Surg, 113, pp. 585-594.
19. Zuk, PA, Zhu, M. and Ashjian, P. (2002) Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell, 13, pp. 4279-4295.