An shRNA silencing a non-toxic transgene reduces nutrient consumption and increases production of adenoviral vectors in a novel packaging cell

Journal of Cellular Physiology

Volumn 219, Issue 2, 2009, Pages 365-371

  Wang, Lifu ^a   Qi, Xiaoguang ^a   Shen, Ruizhe ^a   Sun, Ying ^b   Tuveson, David A ^c  

^a SHANGHAI JIAO TONG UNIVERSITY SCHOOL OF MEDICINE   (China)

^b YALE UNIVERSITY   (United States)

^c UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOVIRUS VECTOR; SHORT HAIRPIN RNA;

ANIMAL CELL; ARTICLE; CELL LINE; CELL METABOLISM; CONTROLLED STUDY; GENE; GENE EXPRESSION; GENE SILENCING; HLCON GENE; NONHUMAN; NUCLEOTIDE SEQUENCE; NUTRIENT UPTAKE; PACKAGING CELL; PRIORITY JOURNAL; TRANSGENE; ADENOVIRUS; ANIMAL; FOOD; GENE VECTOR; GENETICS; HUMAN; METABOLISM; MOLECULAR GENETICS; RNA INTERFERENCE;

ADENOVIRIDAE; ANIMALS; BASE SEQUENCE; CELL LINE; FOOD; GENETIC VECTORS; HUMANS; MOLECULAR SEQUENCE DATA; RNA INTERFERENCE; TRANSGENES;

EID: 62149132180     PISSN: 00219541     EISSN: None     Source Type: Journal    
DOI: 10.1002/jcp.21679     Document Type: Article

References (16)

1. Brummelkamp TR, Bernards R, Agami R. 2002. A system for stable expression of short interfering RNAs in mammalian cells. Science 296:550-553.
2. Brunetti-Pierri N, Ng P. 2008. Progress and prospects: Gene therapy for genetic diseases with helper-dependent adenoviral vectors. Gene Ther 15:553-560.
3. He TC, Zhou S, da Costa LT, Yu J, Kinzler KW, Vogelstein B. 1998. A simplified system for generating recombinant adenoviruses. Proc Natl Acad Sci USA 95:2509-2514.
4. Hingorani SR, Wang L, Multani AS, Combs C, Deramaudt TB, Hruban RH, Rustgi AK, Chang S, Tuveson DA. 2005. Trp53R172H and KrasG12D cooperate to promote chromosomal instability and widely metastatic pancreatic ductal adenocarcinoma in mice. Cancer Cell 7:469-483.
5. Hu Z, Sun Y, Garen A. 1999. Targeting tumor vasculature endothelial cells and tumor cells for immunotherapy of human melanoma in a mouse xenograft model. Proc Natl Acad Sci USA 96:8161-8166.
6. Kretschmer-Kazemi Far R, Sczakiel G. 2003. The activity of siRNA in mammalian cells is related to structural target accessibility: A comparison with antisense oligonucleotides. Nucleic Acids Res 31:4417-4424.
7. Larregina AT, Morelli AE, Dewey RA, Castro MG, Fontana A, Lowenstein PR. 1998. FasL induces Fas/Apo1-mediated apoptosis in human embryonic kidney 293 cells routinely used to generate E1-deleted adenoviral vectors. Gene Ther 5:563-568.
8. Luo J, Deng ZL, Luo X, Tang N, Song WX, Chen J, Sharff KA, Luu HH, Haydon RC, Kinzler KW, Vogelstein B, He TC. 2007. A protocol for rapid generation of recombinant adenoviruses using the AdEasy system. Nat Protoc 2:1236-1247.
9. Paddison PJ, Caudy AA, Bernstein E, Hannon GJ, Conklin DS. 2002. Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells. Genes Dev 16:948-958.
10. Reddy PS, Ganesh S, Hawkins L, Idamakanti N. 2007. Generation of recombinant adenovirus using the Escherichia coli BJ5183 recombination system. Methods Mol Med 130:61-68.
11. Sui G, Soohoo C, Affar el B, Gay F, Shi Y, Forrester WC. 2002. A DNA vector-based RNAi technology to suppress gene expression in mammalian cells. Proc Natl Acad Sci USA 99:5515-5520.
12. Tuveson DA, Shaw AT, Willis NA, Silver DP, Jackson EL, Chang S, Mercer KL, Grochow R, Hock H, Crowley D, Hingorani SR, Zaks T, King C, Jacobetz MA, Wang L, Bronson RT, Orkin SH, DePinho RA, Jacks T. 2004. Endogenous oncogenic K-ras(G12D) stimulates proliferation and widespread neoplastic and developmental defects. Cancer Cell 5:375-387.
13. Wang LF, Hingorani SR, Tuveson DA. 2004. Detecting and diagnosing ampullary neoplasms. Cancer Biol Ther 3:657-659.
14. Xia XG, Zhou H, Ding H, Affar el B, Shi Y, Xu Z. 2003. An enhanced U6 promoter for synthesis of short hairpin RNA. Nucleic Acids Res 31:e100.
15. Young LS, Searle PF, Onion D, Mautner V. 2006. Viral gene therapy strategies: From basic science to clinical application. J Pathol 208:299-318.
16. Yu JY, DeRuiter SL, Turner DL. 2002. RNA interference by expression of short-interfering RNAs and hairpin RNAs in mammalian cells. Proc Natl Acad Sci USA 99:6047-6052.