Mass spectrometric analysis of the S-layer proteins from Clostridium difficile demonstrates the absence of glycosylation

Journal of Mass Spectrometry

Volumn 44, Issue 3, 2009, Pages 368-374

  Qazi, Omar ^b,c   Hitchen, Paul ^c   Tissot, Bérangère ^c   Panico, Maria ^c   Morris, Howard R ^c,d   Dell, Anne ^c   Fairweather, Neil ^a,c  

^a Division of Cell and Molecular Biology   (United Kingdom)

^b University of Texas Medical Branch School of Medicine   (United States)

^c IMPERIAL COLLEGE LONDON   (United Kingdom)

^d M SCAN Ltd   (United Kingdom)

Author keywords

Bacterium; Clostridium difficile; Glycosylation; Pathogenesis; S layer

Indexed keywords

BACTERIAL CELL SURFACES; BACTERIAL SPECIES; CELL WALLS; CLOSTRIDIUM DIFFICILE; CYANOGEN BROMIDES; DIFFICILE STRAINS; GLYCANS; GLYCOSYLATED; HIGH-MOLECULAR WEIGHTS; IN-DEPTH STUDIES; LOW MOLECULAR WEIGHTS; MALDI-TOF; MASS SPECTROMETRIC ANALYSIS; MATRIX-ASSISTED LASERS; PATHOGENESIS; POST-TRANSLATIONAL MODIFICATIONS; S-LAYER; S-LAYER PROTEINS; TIME OF FLIGHTS; TRYPTIC PEPTIDES;

AMINES; BACTERIOLOGY; CELL MEMBRANES; ESTERIFICATION; GLYCOSYLATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETERS; MASS SPECTROMETRY;

PROTEINS;

CYANOGEN BROMIDE; GLYCAN; PEPTIDE; PROTEASOME; VITRONECTIN;

ARTICLE; BACTERIAL CELL; BACTERIAL STRAIN; CELL WALL; CLOSTRIDIUM DIFFICILE; GLYCOSYLATION; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR WEIGHT; NONHUMAN; PRIORITY JOURNAL;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; CLOSTRIDIUM DIFFICILE; CYANOGEN BROMIDE; ESCHERICHIA COLI; GLYCOSYLATION; MEMBRANE GLYCOPROTEINS; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; PEPTIDE FRAGMENTS; RECOMBINANT PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TRYPSIN;

BACTERIA (MICROORGANISMS); CLOSTRIDIUM DIFFICILE;

EID: 62149118280     PISSN: 10765174     EISSN: 10969888     Source Type: Journal    
DOI: 10.1002/jms.1514     Document Type: Article

References (21)

1. I. R. Poxton, J. McCoubrey, G. Blair. The pathogenicity of Clostridium difficile. Clinical Microbiology and Infection 2001, 7, 421.
2. I. Just, R. Gerhard. Large clostridial cytotoxins. Reviews of Physiology Biochemistry and Pharmacology 2004, 152, 23.
3. D. E. Voth, J. D. Ballard. Clostridium difficile toxins: mechanism of action and role in disease. Clinical Microbiology Reviews 2005, 18, 247.
4. J. Pizarro-Cerda, P. Cossart. Bacterial adhesion and entry into host cells. Cell 2006, 124, 715.
5. U. B. Sleytr, M. Sara. Bacterial and archaeal S-layer proteins: structure-function relationships and their biotechnological applications. Trends in Biotechnology 1997, 15, 20.
6. M. Sara, U. B. Sleytr. S-layer proteins. Journal of Bacteriology 2000, 182, 859.
7. B. Kuen, U. B. Sleytr, W. Lubitz. Sequence analysis of the sbsA gene encoding the 130-kDa surface-layer protein of Bacillus stearothermophilus strain PV72. Gene 1994, 145, 115.
8. E. Calabi, S. Ward, B. Wren, T. Paxton, M. Panico, H. Morris, A. Dell, G. Dougan, N. Fairweather. Molecular characterization of the surface layer proteins from Clostridium difficile. Molecular Microbiology 2001, 40, 1187.
9. T. Kar alainen, N. Saumier, M-C Barc, M. Delmee, A. Collignon. Clostridium difficile genotyping based on slpA variable region in S-layer gene sequence: an alternative to serotyping. Journal of Clinical Microbiology 2002, 40, 2452.
10. D. Eidhin, A. Ryan, R. Doyle, J. B. Walsh, D. Kelleher. Sequence and phylogenetic analysis of the gene for surface layer protein, slpA, from 14 PCR ribotypes of Clostridium difficile. Journal of Medical Microbiology 2006, 55, 69.
11. E. Calabi, F. Calabi, A. D. Phillips, N. Fairweather. Binding of Clostridium difficile surface layer proteins to gastrointestinal tissues. Infection and Immunity 2002, 70, 5770.
12. C. Schaffer, P. Messner. Surface-layer glycoproteins: an example for the diversity of bacterial glycosylation with promising impacts on nanobiotechnology. Glycobiology 2004, 14, 31R.
13. C. Schaffer, P. Messner. Glycobiology of surface layer proteins. Biochimie 2001, 83, 591.
14. M. Cerquetti, A. Pantosti, P. Stefanelli, P. Mastrantonio. Purification and characterization of an immunodominant 36 kDa antigen present on the cell surface of Clostridium difficile. Microbial Pathogenesis 1992, 13, 271.
15. M. Cerquetti, A. Molinari, A. Sebastianelli, M. Diociaiuti, R. Petruzzelli, C. Capo, P. Mastrantonio. Characterization of surface layer proteins from different Clostridium difficile clinical isolates. Microbial Pathogenesis 2000, 28, 363.
16. P. L. Mauri, P. G. Pietta, A. Maggioni, M. Cerquetti, A. Sebastianelli, P. Mastrantonio. Characterization of surface layer proteins from Clostridium difficile by liquid chromatography/electrospray ionization mass spectrometry. Rapid Communications in Mass Spectrometry 1999, 13, 695.
17. E. Calabi, N. Fairweather. Patterns of sequence conservation in the S-layer proteins and related sequences in Clostridium difficile. Journal of Bacteriology 2002, 184, 3886.
18. R. A. Stabler, D. N. Gerding, J. G. Songer, D. Drudy, J. S. Brazier, H. T. Trinh, A. A. Witney, J. Hinds, B. W. Wren. Comparative phylogenomics of Clostridium difficile reveals clade specificity and microevolution of hypervirulent strains. Journal of Bacteriology 2006, 188, 7297.
19. J. Ganeshapillai, E. Vinogradov, J. Rousseau, J. S. Weese, M. A. Monteiro. Clostridium difficile cell-surface polysaccharides composed of pentaglycosyl and hexaglycosyl phosphate repeating units. Carbohydrate Research 2008, 343, 703.
20. U. B. Sleytr, H. Bayley, M. Sara, A. Breitwieser, S. Kupcu, C. Mader, S. Weigert, F. M. Unger, P. Messner, B. Jahn-Schmid, B. Schuster, D. Pum, K. Douglas, N. A. Clark, J. T. Moore, T. A. Winningham, S. Levy, I. Frithsen, J. Pankovc, P. Beale, H. P. Gillis, D. A. Choutov, K. P. Martin. Applications of S-layers. FEMS Microbiology Reviews 1997, 20, 151.
21. B. Schuster, D. Pum, M. Sara, U. B. Sleytr. S-layer proteins as key components of a versatile molecular construction kit for biomedical nanotechnology. Mini-Reviews in Medicinal Chemistry 2006, 6, 909.