Incorporation of ceramides into Saccharomyces cerevisiae glycosylphosphatidylinositol-anchored proteins can be monitored in vitro

Eukaryotic Cell

Volumn 8, Issue 3, 2009, Pages 306-314

  Bosson, Régine ^a   Guillas, Isabelle ^a,c,d   Vionnet, Christine ^a   Roubaty, Carole ^a   Conzelmann, Andreas ^a,b  

^a UNIVERSITY OF FRIBOURG   (Switzerland)

^b Division of Biochemistry   (Switzerland)

^c SORBONNE UNIVERSITÉ   (France)

^d CNRS   (France)

Author keywords

[No Author keywords available]

Indexed keywords

CERAMIDE; GLYCOSYLPHOSPHATIDYLINOSITOL; SACCHAROMYCES CEREVISIAE PROTEIN;

ARTICLE; GENETICS; METABOLISM; SACCHAROMYCES CEREVISIAE;

CERAMIDES; GLYCOSYLPHOSPHATIDYLINOSITOLS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

BOVINAE; SACCHAROMYCES CEREVISIAE;

EID: 61949392673     PISSN: 15359778     EISSN: None     Source Type: Journal    
DOI: 10.1128/EC.00257-08     Document Type: Article

References (36)

1. Awaya, J., T. Ohno, H. Ohno, and S. Omura. 1975. Substitution of cellular fatty acids in yeast cells by the antibiotic cerulenin and exogenous fatty acids. Biochim. Biophys. Acta 409:267-273.
2. Bertello, L. E., M. J. Alves, W. Colli, and R. M. de Lederkremer. 2004. Inositolphosphoceramide is not a substrate for the first steps in the biosynthesis of glycoinositolphospholipids in Trypanosoma cruzi. Mol. Biochem. Parasitol. 133:71-80.
3. Bosson, R., and A. Conzelmann. 2007. Multiple functions of inositolphosphorylceramides in the formation and intracellular transport of glycosylphosphatidylinositol-anchored proteins in yeast. Biochem. Soc. Symp. 74:199-209.
4. Bosson, R., M. Jaquenoud, and A. Conzelmann. 2006. GUP1 of Saccharomyces cerevisiae encodes an O-acyltransferase involved in remodeling of the GPI anchor. Mol. Biol. Cell 17:2636-2645.
5. Cerantola, V., C. Vionnet, O. F. Aebischer, T. Jenny, J. Knudsen, and A. Conzelmann. 2007. Yeast sphingolipids do not need to contain very long chain fatty acids. Biochem. J. 401:205-216.
6. Conzelmann, A., C. Fankhauser, and C. Desponds. 1990. Myoinositol gets incorporated into numerous membrane glycoproteins of Saccharomyces cerevisiae; incorporation is dependent on phosphomannomutase (sec53). EMBO J. 9:653-661.
7. Conzelmann, A., A. Puoti, R. L. Lester, and C. Desponds. 1992. Two different types of lipid moieties are present in glycophosphoinositol-anchored membrane proteins of Saccharomyces cerevisiae. EMBO J. 11:457-466.
8. Dickson, R. C., E. E. Nagiec, M. Skrzypek, P. Tillman, G. B. Wells, and R. L. Lester. 1997. Sphingolipids are potential heat stress signals in Saccharomyces. J. Biol. Chem. 272:30196-30200.
9. Fankhauser, C., S. W. Homans, J. E. Thomas-Oates, M. J. McConville, C. Desponds, A. Conzelmann, and M. A. Ferguson. 1993. Structures of glycosylphosphatidylinositol membrane anchors from Saccharomyces cerevisiae. J. Biol. Chem. 268:26365-26374.
10. Ferguson, M. A., S. W. Homans, R. A. Dwek, and T. W. Rademacher. 1988. Glycosyl-phosphatidylinositol moiety that anchors Trypanosoma brucei variant surface glycoprotein to the membrane. Science 239:753-759.
11. Fontaine, T., T. K. Smith, A. Crossman, J. S. Brimacombe, J. P. Latge, and M. A. Ferguson. 2004. In vitro biosynthesis of glycosylphosphatidylinositol in Aspergillus fumigatus. Biochemistry 43:15267-15275.
12. Fujita, M., and Y. Jigami. 2008. Lipid remodeling of GPI-anchored proteins and its function. Biochim. Biophys. Acta 1780:410-420.
13. Fujita, M., T. Yoko-O, and Y. Jigami. 2006. Inositol deacylation by Bst1p is required for the quality control of glycosylphosphatidylinositol- anchored proteins. Mol. Biol. Cell 17:834-850.
14. Ghugtyal, V., C. Vionnet, C. Roubaty, and A. Conzelmann. 2007. CWH43 is required for the introduction of ceramides into GPI anchors in Saccharomyces cerevisiae. Mol. Microbiol. 65:1493-1502.
15. Guillas, I., P. A. Kirchman, R. Chuard, M. Pfefferli, J. C. Jiang, S. M. Jazwinski, and A. Conzelmann. 2001. C26-CoA-dependent ceramide synthesis of Saccharomyces cerevisiae is operated by Lag1p and Lac1p. EMBO J. 20:2655-2665.
16. Guillas, I., M. Pfefferli, and A. Conzelmann. 2000. Analysis of ceramides present in glycosylphosphatidylinositol anchored proteins of Saccharomyces cerevisiae. Methods Enzymol. 312:506-515.
17. Haak, D., K. Gable, T. Beeler, and T. Dunn. 1997. Hydroxylation of Saccharomyces cerevisiae ceramides requires Sur2p and Scs7p. J. Biol. Chem. 272:29704-29710.
18. Horvath, A., C. Sutterlin, U. Manning-Krieg, N. R. Movva, and H. Riezman. 1994. Ceramide synthesis enhances transport of GPI-anchored proteins to the Golgi apparatus in yeast. EMBO J. 13:3687-3695.
19. Jaquenoud, M., M. Pagac, A. Signorell, M. Benghezal, J. Jelk, P. Butikofer, and A. Conzelmann. 2008. The Gup1 homologue of Trypanosoma brucei is a GPI glycosylphosphatidylinositol remodelase. Mol. Microbiol. 67:202-212.
20. Magnuson, K., S. Jackowski, C. O. Rock, and J. E. J. Cronan. 1993. Regulation of fatty acid biosynthesis in Escherichia coli. Microbiol. Rev. 57:522-542.
21. Martin-Yken, H., A. Dagkessamanskaia, P. De Groot, A. Ram, F. Klis, and J. Francois. 2001. Saccharomyces cerevisiae YCRO17c/CWH43 encodes a putative sensor/transporter protein upstream of the BCK2 branch of the PKC1-dependent cell wall integrity pathway. Yeast 18:827-840.
22. Mitchell, A. G., and C. E. Martin. 1997. Fah1p, a Saccharomyces cerevisiae cytochrome b5 fusion protein, and its Arabidopsis thaliana homolog that lacks the cytochrome b5 domain both function in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids. J. Biol. Chem. 272:28281-28288.
23. Miyake, Y., Y. Kozutsumi, S. Nakamura, T. Fujita, and T. Kawasaki. 1995. Serine palmitoyltransferase is the primary target of a sphingosine-like immunosuppressant, ISP-1/myriocin. Biochem. Biophys. Res. Commun. 211:396-403.
24. Nagiec, M. M., E. E. Nagiec, J. A. Baltisberger, G. B. Wells, R. L. Lester, and R. C. Dickson. 1997. Sphingolipid synthesis as a target for antifungal drugs. Complementation of the inositol phosphorylceramide synthase defect in a mutant strain of Saccharomyces cerevisiae by the AUR1 gene. J. Biol. Chem. 272:9809-9817.
25. Natter, K., P. Leitner, A. Faschinger, H. Wolinski, S. McCraith, S. Fields, and S. D. Kohlwein. 2005. The spatial organization of lipid synthesis in the yeast Saccharomyces cerevisiae derived from large scale green fluorescent protein tagging and high resolution microscopy. Mol. Cell Proteomics 4:662-672.
26. Pittet, M., and A. Conzelmann. 2007. Biosynthesis and function of GPI proteins in the yeast Saccharomyces cerevisiae. Biochim. Biophys. Acta 1771:405-420.
27. Pittet, M., D. Uldry, M. Aebi, and A. Conzelmann. 2006. The N-glycosylation defect of cwh8Delta yeast cells causes a distinct defect in sphingolipid biosynthesis. Glycobiology 16:155-164.
28. Reggiori, F., E. Canivenc-Gansel, and A. Conzelmann. 1997. Lipid remodeling leads to the introduction and exchange of defined ceramides on GPI proteins in the ER and Golgi of Saccharomyces cerevisiae. EMBO J. 16:3506-3518.
29. Schneiter, R., B. Brugger, R. Sandhoff, G. Zellnig, A. Leber, M. Lampl, K. Athenstaedt, C. Hrastnik, S. Eder, G. Daum, F. Paltauf, F. T. Wieland, and S. D. Kohlwein. 1999. Electrospray ionization tandem mass spectrometry (ESI-MS/MS) analysis of the lipid molecular species composition of yeast subcellular membranes reveals acyl chain-based sorting/remodeling of distinct molecular species en route to the plasma membrane. J. Cell Biol. 146:741-754.
30. Sherman, F. 2002. Getting started with yeast. Methods Enzymol. 350:3-41.
31. Sipos, G., F. Reggiori, C. Vionnet, and A. Conzelmann. 1997. Alternative lipid remodelling pathways for glycosylphosphatidylinositol membrane anchors in Saccharomyces cerevisiae. EMBO J. 16:3494-3505.
32. Smith, S. W., and R. L. Lester. 1974. Inositol phosphorylceramide, a novel substance and the chief member of a major group of yeast sphingolipids containing a single inositol phosphate. J. Biol. Chem. 249:3395-3405.
33. Sutterlin, C., T. L. Doering, F. Schimmoller, S. Schroder, and H. Riezman. 1997. Specific requirements for the ER to Golgi transport of GPI-anchored proteins in yeast. J. Cell Sci. 110:2703-2714.
34. Umemura, M., M. Fujita, T. Yoko-O, A. Fukamizu, and Y. Jigami. 2007. Saccharomyces cerevisiae CWH43 is involved in the remodeling of the lipid moiety of GPI anchors to ceramides. Mol. Biol. Cell 18:4304-4316.
35. Watanabe, R., K. Funato, K. Venkataraman, A. H. Futerman, and H. Riezman. 2002. Sphingolipids are required for the stable membrane association of glycosylphosphatidylinositol-anchored proteins in yeast. J. Biol. Chem. 277:49538-49544.
36. Zhu, Y., C. Vionnet, and A. Conzelmann. 2006. Ethanolaminephosphate side chain added to glycosylphosphatidylinositol (GPI) anchor by mcd4p is required for ceramide remodeling and forward transport of GPI proteins from endoplasmic reticulum to Golgi. J. Biol. Chem. 281:19830-19839.