Development of a Most-probable-number Method for Enumerating Denitrifying Bacteria by Using 96-Well Microtiter Plates and an Anaerobic Culture System

Microbes and Environments

Volumn 18, Issue 4, 2003, Pages 210-215

  Saitoh, Satoshi ^a,b,d   Iwasaki, Kazuhiro ^c   Yagi, Osami ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

^c NATIONAL INSTITUTE FOR ENVIRONMENTAL STUDIES   (Japan)

^d TAKENAKA CORPORATION   (Japan)

Author keywords

denitrifying bacteria; enumeration; microtiter plate; most probable number

Indexed keywords

EID: 61949165393     PISSN: 13426311     EISSN: 13474405     Source Type: Journal    
DOI: 10.1264/jsme2.18.210     Document Type: Article

References (26)

1. Binnerup, S.J., K. Jensen, N.P. Revsbech, M.H. Jensen and J. Sørensen. 1992. Denitrification, dissimilatory reduction of nitrate to ammonium, and nitrification in a bioturbated estuarine sediment as measured with 15N and microsensor techniques. Appl. Environ. Microbiol. 58: 303–313.
2. Blanc, M., L. Marilley, T. Beffa and M. Aragno. 1999. thermophilic bacterial communities in hot composts as revealed by most probable number counts and molecular (16S rDNA) methods. FEMS Microbiol. Ecol. 28: 141–149.
3. Brown, E.J. and J.F. Braddock. 1990. Sheen screen, a miniaturized most-probable-number method for enumeration of oil-degrading microorganisms. Appl. Environ. Microbiol. 56: 3895–3896.
4. Costa, C., C. Dijkema, M. Friedrich, P. García-Encina, F. Fernández-Polanco and A.J.M. Stams. 2000. Denitrification with methane as electron donor in oxygen-limited bioreactors. Appl. Microbiol. Biotechnol. 53: 754–762.
5. Davidson, E.A., M.K. Strand and L.F. Galloway. 1985. Evaluation of the most probable number method for enumerating denitrifying bacteria. Soil Sci. Soc. Am. J. 49: 642–645.
6. Etchebehere, C., I. Errazquin, E. Barrandeguy, P. Dabert, R. Moletta and L. Muxí. 2001. Evaluation of the denitrifying microbiota of anoxic reactors. FEMS Microbiol. Ecol. 35: 259–265.
7. Gamble, T.N., M.R. Betlach and J.M. Tiedje. 1977. Numerically dominant denitrifying bacteria from world soils. Appl. Environ. Microbiol. 33: 926–939.
8. Gliesche, C.G. and A. Fesefeldt. 1998. Monitoring the denitrifying Hyphomicrobium DNA/DNA hybridization group HG 27 in activated sludge and lake water using MPN cultivation and subsequent screening with the gene probe Hvu-1. Syst. Appl. Microbiol. 21: 315–320.
9. Goldberg, V.M. 1989. Groundwater pollution by nitrates from livestock wastes. Environ. Health Perspect. 83: 25–29.
10. Haines, J.R., B.A. Wrenn, E.L. Holder, K.L. Strohmeier, R.T. Herrington and A.D. Venosa. 1996. Measurement of hydrocarbon-degrading microbial populations by a 96-well plate most-probable-number procedure. J. Ind. Microbiol. 6: 36–41.
11. Hartel, P.G. and C. Hagedorn. 1983. Microtechnique for isolating fecal coliforms from soil. Appl. Environ. Microbiol. 46: 518–520.
12. Jensen, K., N.P. Revsbech and L.P. Nielsen. 1993. Microscale distribution of nitrification activity in sediment determined with a shielded microsensor for nitrate. Appl. Environ. Microbiol. 59: 3287–3296.
13. Jones, A.M. and R. Knowles. 1991. Enumeration of Flexibacter canadensis in environmental samples by using a bacteriophage isolated from soil. Appl. Environ. Microbiol. 57: 3043–3045.
14. Klee, A.J. 1993. A computer program for the determination of most probable number and its confidence limits. J. Microbiol. Methods. 18: 91–98.
15. Klemedtsson, L., Q. Jiang, Å.K. Klemedtsson and L. Bakken. 1999. Autotrophic ammonium-oxidising bacteria in Swedish mor humus. Soil Biol. Biochem. 31: 839–847.
16. Knowles, R. 1982. Denitrification. Microbiol. Rev. 46: 43–70.
17. Mahne, I. and J.M. Tiedje. 1995. Criteria and methodology for identifying respiratory denitrifiers. Appl. Environ. Microbiol. 61: 1110–1115.
18. Maul, A. and J.C. Block. 1983. Microplate fecal coliform method to monitor stream water pollution. Appl. Environ. Microbiol. 46: 1032–1037.
19. Neef, A., A. Zaglauer, H. Meier, R. Amann, H. Lemmer and K. H. Schleifer. 1996. Population analysis in a denitrifying sand filter: conventional and in situ identification of Paracoccus spp. in methanol-fed biofilms. Appl. Environ. Microbiol. 62: 4329- 4339.
20. Nokhal, T. H. and H.G. Schlege. 1983. Taxonomic study of Paracoccus denitrificans. Int. J. Syst. Bacteriol. 33: 26–37.
21. Rodhe, H. 1990. A comparison of the contribution of various gases to the greenhouse effect. Science. 248: 1217–1219.
22. Rowe, R., R. Todd and J. Waide. 1977. Microtechnique for most-probable-number analysis. Appl. Environ. Microbiol. 33: 675–680.
23. Tiedje, J.M. 1982. Denitrification, p. 1011–1026. In A.L. Page, R.H. Miller and D.R. Keeney (ed.), Methods of soil analysis, Part 2. Chemical and Microbiological Properties, Agronomy Monograph no. 9, 2nd ed. ASA-SSSA, Madison, WI.
24. Watahiki, M., S. Hata and T. Aida. 1983. N2O accumulation and inhibition of N2O reduction by denitrifying Pseudomonas sp. 220A in the presence of oxygen. Agric. Biol. Chem. 47: 1991- 1996.
25. Wrenn, B.A. and A.D. Venosa. 1996. Selective enumeration of aromatic and aliphatic hydrocarbon degrading bacteria by a most-probable-number procedure. Can. J. Microbiol. 42: 252–258.
26. Yoshinari, T., R. Hynes and R. Knowles. 1977. Acetylene inhibition of nitrous oxide reduction and measurement of denitrification and nitrogen fixation in soil. Soil Biol. Biochem. 9: 177–183.