Monitoring the denitrifying Hyphomicrobium DNA/DNA hybridization group HG 27 in activated sludge and lake water using MPN cultivation and subsequent screening with the gene probe Hvu-1

Systematic and Applied Microbiology

Volumn 21, Issue 2, 1998, Pages 315-320

  Gliesche, Christian G ^a   Fesefeldt, Andreas ^a  

^a UNIVERSITY OF KIEL   (Germany)

Author keywords

Activated sludge; Denitrification; Gene probe; Hyphomicrobium; Methanol dehydrogenase; Methylotrophic bacteria

Indexed keywords

BACTERIAL DNA; LAKE WATER;

ACTIVATED SLUDGE; ANAEROBIC BACTERIUM; ARTICLE; BACTERIUM CULTURE; DENITRIFICATION; DNA HYBRIDIZATION; DNA PROBE; ECOSYSTEM; HYPHOMICROBIUM; NITRIFICATION; NONHUMAN; PRIORITY JOURNAL; SEASONAL VARIATION;

BACTERIA (MICROORGANISMS); HYPHOMICROBIUM; NEGIBACTERIA;

EID: 0031820403     PISSN: 07232020     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0723-2020(98)80039-8     Document Type: Article

References (25)

1. AA, K., OLSEN, R. A.: The use of various substrates and substrate concentrations by a Hyphomicrobium sp. isolated from soil: Effect on growth rate and growth yield. Microb. Ecol. 31, 67-76 (1996).
2. AMARAL, J. A., ARCHAMBAULT, C., RICHARDS, S. R., KNOWLES, R.: Denitrification associated with groups I and II methanotrophs in a gradient enrichment system. FEMS Microb. Ecol. 18, 289-298 (1995).
3. BLOEM, J.: Fluorescent staining of microbes for total direct counts, pp. 2.6.1:1-12. In: Molecular Microbial Ecology Manual (A. D. L AKKERMANS, J. D. VAN ELSAS, and F. J. DE BRUIJN, eds.). AA Dordrecht, The Netherlands, Kluwer Academic Publishers (1995).
4. CLAUS, G., KUTZNER, H. J.: Denitrification of nitric acid with methanol as carbon source. Appl. Microbiol. Biotechnol. 22:378-381 (1985).
5. COLLINS, V. G., WILLOUGHBY, L. G.: The distribution of bacteria and fungal spores in Blelham Tarn with particular reference to an experimental overturn. Arch. Microbiol. 43, 294-307 (1962).
6. FESEFELDT, A., GLIESCHE, C. G.: Identification of Hyphomicrobium spp. using PCR-amplified fragments of the mxaF gene as a molecular marker. System. Appl. Microbiol. 20, 387-396 (1997).
7. FESEFELDT, A., POETSCH, M., GLIESCHE, C. G.: Development of a species-specific gene probe for Hyphomicrobium facilis using the inverse polymerase chain reaction. Appl. Environ. Microbiol. 63, 335-337 (1997).
8. GLIESCHE, C. G, HIRSCH, P.: Mutagenesis and chromosome mobilization in Hyphomicrobium facilis B-522. Can. J. Microbiol. 38, 1167-1174 (1992).
9. GLIESCHE, C. G., MENZEL, M., FESEFELDT, A.: A rapid method for creating species-specific gene probes for methylotrophic bacteria. J. Microbiol. Methods 28, 25-34 (1997).
10. GRASSHOFF, G., EHRHARDT, M., KREMLING, K.: Methods of Seawater Analysis, 2nd ed.. Weinheim, Verlag Chemie (1983).
11. HIRSCH, P.: Genus Hyphomicrobium Stutzer and Hartleb 1898, 76AL*, pp. 1895-1904. In: Bergey's manual of systematic bacteriology, vol. 3. (J. T. STALEY, M. P. BRYANT, N. PFENNIG and J. G. HOLT, eds.). Baltimore, Williams and Wilkins (1989).
12. HIRSCH, P. R.: Detection of microbial DNA sequences by colony hybridization, pp. 2.6.1:1-12. In: Molecular Microbial Ecology Manual (A. D. L AKKERMANS, J. D. VAN ELSAS, and F. J. DE BRUIJN, eds.). AA Dordrecht, The Netherlands, Kluwer Academic Publishers (1995).
13. HOLM, N. C., GLIESCHE, C. G., HIRSCH, P.: Diversity and structure of Hyphomicrobium populations in a sewage treatment plant and its adjacent receiving lake. Appl. Environ. Microbiol. 62, 522-528 (1996).
14. KLOOS, K., FESEFELDT, A., GLIESCHE, C. G., BOTHE, H.: DNA-probing indicates the occurrence of denitrification and nitrogen fixation genes in Hyphomicrobium: Distribution of denitrifying and nitrogen fixing isolates of Hyphomicrobium in a sewage treatment plant. FEMS Microbiol. Ecol. 18, 205-213 (1995).
15. LIESSENS, J.: Removing nitrate with a methylotrophic fluidized bed: microbiological quality. J. Am. Wat. Assoc. 85, 155-161 (1993).
16. LIN, Y.-F., CHEN, K.-C.: Denitrification and methanogenesis in a co-immobilized mixed culture system. Wat. Res. 29, 35-43 (1995).
17. NEEF, A., ZAGLAUER, A., MEIER, H., AMANN, R., LEMMER, H., SCHLEIFER, K.-H.: Population analysis in a denitrifying sand filter: conventional and in situ identification of Paracoccus spp. in methanol-fed biofilms. Appl. Environ. Microbiol. 62, 4329-4339 (1996).
18. NYBERG, U., ASPEGREN, H., ANDERSSON, B., JANSEN, J., VILLADSEN, I. S.: Full-scale application of nitrogen removal with methanol as carbon source. Wat. Sci. Tech. 26, 1077-1086 (1992).
19. POINDEXTER, J. S.: Dimorphic prosthecate bacteria: the genera Caulobacter, Asticcacaulis, Hyphomicrobium, Pedomicrobium, Hyphomonas, and Thiodendron, pp 2176-2196. In: The Prokaryotes: a Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications, 2nd ed. (A. BALOWS, H. G. TRÜPER, M. DWORKIN, W. HARDER, K.-H. SCHLEIFER, eds.). New York, Springer (1992).
20. SAMBROOK, J. E., FRITSCH, F., MANIATIS, T.: Molecular cloning: A Laboratory Manual, vol. 1-3. Cold Spring Harbor, NY, Cold Spring Harbor Laboratory Press (1989).
21. STALEY, J. T.: Prosthecomicrobium and Ancalomicrobium, new prosthecate freshwater bacteria. J. Bacteriol. 95, 1921-1942 (1968).
22. URAKAMI, T., SASAKI, J., SUZUKI, K.-I., KOMAGATA, K.: Characterization and description of Hyphomicrobium denitrificans sp. nov.. Int. J. Syst. Bacteriol. 45, 528-532 (1995).
23. VEDENINA, J. Y., GOVORUKHINA, N. I.: Formation of methylotrophic denitrifying coenosis in a sewage purification system for removal of nitrates. Mikrobiologiya 57, 320-328 (engl. transl. 261-268) (1988).
24. WILKINSON, T. G., TOPIWALA H. H., HAMER, G.: Interactions in a mixed bacterial population growing on methane in continuous culture. Biotechnol. Bioeng. 16, 41-59 (1974).
25. ZELLNER, G., FEUERHAKE, E., JÖRDENING, H.-J., MACARIO, A. J. L., CONWAY DE MACARIO, E.: Denitrifying and methanogenic bacteria in the biofilm of a fixed-film reactor operated with methanol/nitrate demonstrated by immunofluorescence and microscopy. Appl. Microbiol. Biotechnol. 43, 566-571 (1995).